Delayed fertilization and short-term storage methods affects the viability of stripped eggs of African catfish Clarias gariepinus
DOI:
https://doi.org/10.36253/caryologia-3424Keywords:
African catfish, Egg storage, Breeding parameters, Saline water, RefrigerationAbstract
The fertilization of eggs may be delayed during induced fish breeding due to unforeseen circumstances. It is, therefore, necessary to know the optimal delay period and explore short-term storage options that can extend the viability of the eggs in such instances. In two studies, African catfish Clarias gariepinus eggs were obtained from broodstocks induced using ovaprim® hormone at 0.5ml/kg. A batch of the stripped eggs was then exposed in triplicate to atmospheric oxygen for 0, 1, 2, 3, 4, 8 and 12 hours post-stripping (HPS). In contrast, another batch was stored for 8 hours using sole and combined methods of “refrigeration”, “addition of extender” and “aeration”. The eggs were then fertilized using fresh sperm in all instances. In both experiments, egg characteristics, breeding parameters, and biometric parameters of hatched fry were recorded. Results showed a size reduction in the eggs as the time of exposure to atmospheric oxygen increased. Also, the fertilization and hatchability of eggs were similar to 4HPS; beyond this threshold, the value declined significantly to zero. However, the hatched fry’s biometric parameters showed no significant difference among treatments (P<0.05). The second study showed a poor breeding performance of eggs stored in all the different storage methods tested. Meanwhile, egg sizes and biometric parameters of the few hatched fry show no significant difference among the treatments (P<0.05). It was therefore concluded that the fertilization of stripped C. gariepinus eggs should not be later than 4HPS to optimize the breeding performance of the fish.
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Adebayo, O.T., Lawal, O.A., and Adeoye, O.A. (2015) Aspects of the Biology and Ecology of African Catfish (Clarotes laticeps) in the Lower Ogun River, Nigeria. Journal of Aquatic Sciences 30(1), 35-43.
Adeyemo, O. K., Adeyemo, O. A., Oyeyemi, M. O., and Gbede, S. A. (2007). Effect of semen extenders on the motility and viability of stored African catfish (Clarias gariepinus) spermatozoa. Journal of Applied Science and Environmental Management 11(1): 13-16.
Ataguba G.A., Okomoda V.T., and Onwuka M.C., (2013) Relationship Between Broodstock Weight Combination and Spawning Success in African catfish (Clarias gariepinus). Croat Journal of Fisheries 71:176–181.
Blaxter, J. H., and Hempel, G. (1963). The Influence of Egg Size on Herring Larvae (Clupea harengus). ICES Journal of Marine Science 28(2): 211-240.
Bobe J. and Labbe C., (2010) Egg and Sperm Quantity in Fish. General and Comparative Endocrinology 165: 535-548
Bromage N. R., and Roberts R. J., (1995) Broodstock Management and Egg and Larval Quality. Blackwell Science Company 2121 S (State Avenue, Ames, Iowa 500148300, USA): Iowa State University Press; p. 6-24: 76-93
Dauda AB, Natrah I, Karim M, Kamarudin MS, and Bichi AH, (2018) African Catfish Aquaculture in Malaysia and Nigeria: Status, Trend and Prospects. Fisheries and Aquaculture Journal 9: 237.
Dettlaff T.A., Ginsburg A.S., and O.I. Schmalhausen. (1993) Sturgeon Fishes. Developmental Biology and Aquaculture. Springer-Verlag, Berlin. 300 pp.
FAO, (2020). The State of World Fisheries and Aquaculture 2020. Sustainability in Action. Rome: FAO. https://doi.org/10.4060/ca9229en
Furuita, H., Ohta, H., Unuma, T., Tanaka, H., Kagawa, H., Suzuki, N., and Yamamoto, T. (2003) Biochemical Composition of Eggs in Relation to Egg Quality in the Japanese Eel, Anguilla japonica. Fish Physiology and Biochemistry 29: 37-46.
Harvey, B., and Kelley, R. N. (1984). Short-term Storage of Sarotherodon mossambicus Ova. Aquaculture 37(4): 391-395.
Hassan, A., Okomoda, V. T., and Nurhayati, M. N. (2018) Embryonic Development of Diploid and Triploid Eggs of Clarias gariepinus (Burchell, 1822). Caryologia 71(4): 372-379.
Idahor, K. O., Okunsebor, S. A., Sokunbi, O. A., Osaiyuwu, O. H., Osayande, U. D., Hamza, J., and Isah, N. (2018) Effect of Storage Temperature on African Catfish (Clarias gariepinus Burchell 1822) Milt Quality. International Journal of Innovation Studies in Aquatic Biology and Fisheries 4(1): 7-12.
Jensen, J. O. T., and Alderdice, D. F., (1984). Effect of Temperature on Short-term Storage of Eggs and Sperm of Chum Salmon (Oncorhynchus keta). Aquaculture 37(3): 251-265.
Kalinina, L., Zelenskaya, I., Trufanova, S., and Kalinin, N. (2020) Prospects of Development of Aquaculture in Russia and its Regions. In E3S Web of Conferences Volume 81, p. 01008. EDP Sciences.
Khedkar G.D. and Khedkar C.D. (2003) Encyclopedia of Food Science and Nutrition, Second Edition
Linhart O, Gela D, Rodina M, and Rodriguez GM (2001) Short-term Storage of Ova of Common Carp and Tench in Extenders. Journal Fish Biology 59(3): 616-623.
Megbowon, I., Fashina-Bombata, HA., Akinwale MA., Hammed, AM., Okunade OA., and Mojekwu TO. (2013) Breeding Performance of Clarias gariepinus Obtained from Nigerian Waters. IOSR Journal of Agriculture and Veterinary Science 6(3): 6-9.
Nguenga, D., Teugels, GG., Legendre, M., and Ollevier, F. (2004) Effects of Storage and Incubation Temperature on the Viability of Eggs, Embryos and Larvae in Two Strains of an African Catfish, Heterobranchus longifilis (Siluriformes, Clariidae). Aquaculture Research 35(14): 1358-1369.
Niksirat H, Sarvi K, Mojazi Amiri B, and Hatef A., (2007) Effects of Storage Duration and Storage Media on Initial and Post-eyeing Mortality of Stored Ova of Rainbow Trout Oncorhynchus mykiss. Aquaculture 262(2-4): 528-531.
Okomoda V.T, Koh ICC, Shahreza M.S. (2017) First Report on the Successful Hybridization of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822). Zygote 25(4): 443-452.
Okomoda V.T., Koh ICC, Shahreza M.S., (2018) A Simple Technique for Accurate Estimation of Fertilization Rate with Specific Application to Clarias gariepinus (Burchell, 1822). Journal of Aquaculture Resources 49: 1116e21.
Olaniyi, W. A., and Omitogun, O. G. (2014) Stages in the Early and Larval Development of the African Catfish Clarias gariepinus (Teleostei, Clariidae). Zygote 22(3): 314-330.
Olufeagba S. O. and Okomoda V. T. (2016). Cannibalism and performance evaluation of hybrids between Clarias batrachus and Clarias gariepinus. Croatian Journal of Fisheries 74(3): 124-129.
Olufeagba SO, Okomoda VT, Shaibu G. (2016) Embryogenesis and Early Growth of Pure Strains and Hybrids Between Clarias gariepinus (Burchell, 1822) and Heterobranchus longifilis Valenciennes, 1840. North American Journal of Aquaculture 78: 346-355.
Omoniyi, S.A., Adeyemi, O.A., and Okechi, J.K. (2018) Growth Performance and Survival Rate of African Catfish (Clarotes laticeps) Juveniles Reared in Concrete Tanks. Nigerian Journal of Fisheries 15(2): 233-238.
Rahman, S. M., Alsaquft, A. S., Alkhamis, Y. A., Rahman, M. M., Ahsan, M. N., Mathew, R. T., and Hossain, Q. Z. (2020) Short Term Storage of Asian Walking Catfish (Clarias batrachus linnaeus, 1758) Gametes. Advances in Animal and Veterinary Sciences 8(12): 1394-1401.
Rizzo, E., Godinho, H. P., and Sato, Y. (2003) Short-term Storage of Oocytes from the Neotropical Teleost Fish Prochilodus marggravii. Theriogenology 60(6): 1059-1070.
Rothbard, S., Rubinshtein, I., and Gelman, E. (1996) Storage of Common Carp, Cyprinus carpio Eggs for Short Durations. Aquaculture Research 27(3): 175-181.
Samarin AM, Żarski D, Palińska-Żarska K, Krejszeff S, Blecha M, Kucharczyk D, and Policar T (2017) Invitro Storage of Unfertilized Eggs of the Eurasian Perch and its Effect on Egg Viability Rates and the Occurrence of Larval Malformations. Journal of Animal Sciences Volume 11(1): 78-83.
Samarin AM, Policar T, Lahnsteiner F (2015) Fish Oocyte Ageing and its Effect on Egg Quality. Review on Fisheries Science and Aquaculture 23(3): 302-314.
Suquet M., Dreanno C., Fauvel C., Cosson J. and Billard R., (2000) Cryopreservation of Sperm in Marine Fish. Aquaculture Research 31: 231-243.
Tilahun, G., and Yalew, A. (2024). Cryopreservation and artificial insemination in African Catfish (Clarias gariepinus, Burchell 1822): A review. Journal of Agriculture and Environmental Sciences 9(1): 110-122.
Tacon, A. G. (2020). Trends in global aquaculture and aquafeed production: 2000–2017. Rev. Fish. Sci. Aquac. 28: 43-56. https://doi.org/10.1080/23308249.2019.1649 634
Urbanyi B., Horvath A., Varga Z. and Horvath L., (1999) Effect of Extenders on Sperm Cryopreservation of African Catfish, Clarias gariepinus (Burchell). Aquaculture Research 30: 145-151
Withler, F. C., and Lim, L. C. (1982) Preliminary Observations of Chilled and Deep-frozen Storage of Grouper (Epinephelus tauvina) Sperm. Aquaculture 27(4): 389-392.
Yue K., and Shen Y., (2021) An Overview of Descriptive Technologies for Aquaculture. Journal of Aquaculture and Fisheries 7: 111-112.
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