@article{Pharmawati_Wirasiti_Wrasiati_2022, title={Genotoxic and antigenotoxic potential of encapsulated Enhalus acoroides (L. f.) Royle leaves extract against nickel nitrate}, volume={75}, url={https://riviste.fupress.net/index.php/caryologia/article/view/1571}, DOI={10.36253/caryologia-1571}, abstractNote={<p class="p1">Several environmental pollutants can cause damage to chromosomes, one of which is the heavy metal NiNO<sub>3</sub>. Some plant extracts have antigenotoxic properties that result in a decrease in chromosomal damage. Member of flowering plants that need to be tested is seagrass. One seagrass species is <em>Enhalus acoroides</em> which was found to contain phytochemical compounds. This study aimed to analyse the genotoxic effect and the potential of encapsulated <em>E. acoroides</em> leaf extract as antigenotoxic against nickel nitrate NiNO<sub>3</sub>. The extraction was conducted using a mixture of chloroform and ethanol, and crude extract encapsulated using maltodextrin and tween 80. Chromosomal aberrations were evaluated using the squash technique of <em>Allium cepa</em> var. aggregatum root tips. Triphenyltetrazolium chloride and Evans Blue staining were used to observe mitochondrial and apoptotic activities. The results showed that at higher concentrations (250 ppm and 500 ppm), the encapsulated <em>E. acoroides</em> extract decreased mitotic indices; however, no chromosome aberration observed. NiNO<sub>3</sub> itself induced a genotoxic effect as observed by low mitotic index and a high percentage of chromosome aberration. The modulation of NiNO<sub>3</sub> effect by adding the encapsulated <em>E. acoroides</em> extract at low concentration (100 ppm) increased mitotic index compared to treatment with Ni alone, but did not reduce chromosome aberration. Simultaneous encapsulated <em>E. acoroides</em> extract and Ni treatment, significantly reduced nuclear fragmentation and nuclear lesion. The encapsulated <em>E. acoroides</em> extract can repair several types of nuclear damage but cannot minimise chromosomal damage.</p>}, number={2}, journal={Caryologia}, author={Pharmawati, Made and Wirasiti, Ni Nyoman and Wrasiati, Luh Putu}, year={2022}, month={Sep.}, pages={89–99} }