TY - JOUR AU - Öztürk, Betül Yilmaz PY - 2019/05/10 Y2 - 2024/03/29 TI - Intracellular and extracellular green synthesis of silver nanoparticles using Desmodesmus sp.: their Antibacterial and antifungal effects JF - Caryologia JA - Caryologia VL - 72 IS - 1 SE - Articles DO - 10.13128/cayologia-249 UR - https://riviste.fupress.net/index.php/caryologia/article/view/249 SP - 29-43 AB - <p class="p1">In this study aim was to perform green synthesis of synthesis silver nanoparticles (LAC-AgNPs, RAE-AgNPs and BAE-AgNPs) by using <em>Desmodesmus</em> sp., intracellular and extracellular synthesis methods and to compare the obtained products with physicochemical characterization techniques. The structural, morphological and optical properties of the synthesized nanoparticles were characterized using UV-Vis spectroscopy, TEM, SEM-EDS, FTIR, DLS and zeta potential. These results clearly show that silver nanoparticles (AgNPs) could be synthesized in different sizes and stabilities with various biological materials obtained from <em>Desmodesmus </em>sp. LAC-AgNPs had size of 10-30 nm, RAE-AgNPs had size of 4-8 nm and BAE-AgNPs had size of 3-6 nm. Also, the antibacterial activity of silver nanoparticles synthesized as intracellular and extracellular showed a strong antibacterial effect against pathogens such as <em>Salmonella</em> sp. and <em>Listeria monocytogenes</em>. Additionally, they have effective antifungal activity against <em>Candida parapsilosis</em>. The broth microdilution method was used for examining antibacterial antifungal effect of synthesis AgNPs. The minimum inhibitory concentration against <em>Salmonella</em> sp., <em>Listeria monocytogenesis</em> and <em>Candida parapsilosis</em><span class="Apple-converted-space">&nbsp; </span>were recorded as 3,125 ?l, 1,5625 µl and 0,78125 µl synthesis AgNPs, respectively. As a result, it has thought that different sizes of synthesis AgNPs may have a great potential for biomedical applications.</p> ER -