TY - JOUR AU - Vosough-Mohebbi, Panthea AU - Zahravi, Mehdi AU - Changizi, Mehdi AU - Khaghani, Shahab AU - Shobbar, Zahra-Sadat PY - 2020/04/03 Y2 - 2024/03/28 TI - Identification of the differentially expressed genes of wheat genotypes in response to powdery mildew infection JF - Caryologia JA - Caryologia VL - 73 IS - 2 SE - Articles DO - 10.13128/caryologia-752 UR - https://riviste.fupress.net/index.php/caryologia/article/view/752 SP - 63-72 AB - <p class="p1">Bread wheat (<em>Triticum aestivum</em> L.) is the most widely grown crop worldwide. Powdery mildew caused by fungal pathogen <em>Blumeria graminis</em> is one of the most devastating diseases of wheat. The present study aimed to identify differentially expressed genes and investigate their expression in response to <em>B. graminis</em> in susceptible (Bolani) and resistant (KC2306) wheat genotypes, using publicly&nbsp;available microarray data set and qRT-PCR analysis. A total of 5760 and 5315 probe sets were detected which 5427 and 4630 by adjusted P-value &lt; 0.05 and 168 and 144 genes based on e-value &lt; 1 × 10<sup>–5</sup> cut-off were differentially expressed in susceptible and resistant wheat genotypes, respectively. Among exclusively up regulated genes in the resistant genotype 12 hpi compared to its control, fifteen potential genes that may be responsible for <em>B. graminis</em> inoculation resistance were detected. The results of real time PCR for the candidate genes showed that the genes were upregulated in the resistant genotype 12 hpi compared to its control, which validated the results of microarray analysis. The <em>bZIP</em>, <em>ERF</em>, and <em>ARF1</em> genes may play an important role in <em>B. graminis</em> resistance. The powdery mildew responsive genes identified in the present study will give us a better understanding on molecular mechanisms involved in <em>B. graminis</em> resistance in different wheat genotypes.</p> ER -