TY - JOUR AU - Bhagyanathan, Neethu Kannan AU - Thoppil, John Ernest PY - 2019/12/13 Y2 - 2024/03/29 TI - Active chemical constituents of Cynanchum viminale and its cytotoxic effects via apoptotic signs on Allium cepa root meristematic cells JF - Caryologia JA - Caryologia VL - 72 IS - 3 SE - Articles DO - 10.13128/caryologia-769 UR - https://riviste.fupress.net/index.php/caryologia/article/view/769 SP - 75-86 AB - <p>The present study evaluates the cytotoxic efficacy of methanolic extract of <em>C. viminale</em> on <em>A. cepa</em> root meristematic cells. DAPI staining was used to study the chromosomal aberrations indued by extract of <em>C. viminale</em>. Evans blue staining method was employed to estimate the cell death of root cells of <em>A. cepa</em>. The plant extract was found to impart severe cytological damges, specifically chromosomal aberrations at interphase and prophase stage of cell division. Various apoptotic signs such as apoptotic body formation, nuclear budding, micronucleus, nuclear disintegration, nuclear breakage <em>etc</em>. were observed in meristematic cells of <em>A. cepa</em>. The results suggest the cytotoxic, preferably genotoxic effect of methanolic extract of <em>C. viminale </em>as evidenced by various apoptotic symptoms on <em>A. cepa </em>root cells.</p> ER -