TY - JOUR AU - Fallah, Fahimeh AU - Ghahremaninejad, Farrokh PY - 2021/07/20 Y2 - 2024/03/28 TI - Ploidy level determination of Hedera (Araliaceae) with an emphasis on discussable species (Hedera hibernica) JF - Caryologia JA - Caryologia VL - 74 IS - 1 SE - Articles DO - 10.36253/caryologia-881 UR - https://riviste.fupress.net/index.php/caryologia/article/view/881 SP - 109-116 AB - <p class="p1"><span class="s1">Genome size is a helpful tool for circumscribing taxa at diverse taxonomic degrees (mostly species) and resolving intricate low-level taxonomies. The correct genome size in </span><span class="s2">Hedera </span><span class="s1">(Araliaceae) has long been discussed, and the ploidy levels of some taxa are still unclear. Twelve accessions of </span><span class="s2">Hedera </span><span class="s1">were measured via flow cytometry. Flow cytometry is a relatively rapid, inexpensive, and credible tool. Fresh leaves of </span><span class="s2">Hedera </span><span class="s1">samples and internal reference standard parsley (</span><span class="s2">Petroselinum crispum</span><span class="s1">) were stained with propidium iodide (PI). Flow cytometry measurements showed that for the accessions of 2CV (3.09 - 6.40 pg), the lowest amount of nuclear DNA was 3.09 pg for </span><span class="s2">Hedera crebrescens </span><span class="s1">(So), while the highest amount was 6.40 pg for </span><span class="s2">H. hibernica </span><span class="s1">“Hamilton,” representing a statistically significant difference. According to this study, the new taxon (</span><span class="s2">H. crebrescens</span><span class="s1">) is a diploid, though this taxon was previously considered </span><span class="s2">H. hibernica </span><span class="s1">(tetraploid)</span><span class="s2">.</span></p> ER -