Enhanced morphologic traits and medicinal constituents of octaploids in Satureja mutica, a high-yielding medicinal savory


  • Anahita Shariat Biotechnology Resesarch Department, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran
  • Fatemeh Sefidkonb Medicinal Plants Research Division, Research Institute of Forests and Rangelands of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tehran




octaploid plants, polyploidy, phytochemical properties, genome size


Satureja mutica is a tetraploid and perennial semi-bushy plant cultivated for different medicinal purposes. To induce polyploidy, two-leafed seedlings were exposed to different concentrations (0.00, 0.05, 0.1, and 0.2 % w/v) and durations (6, 12, and 24 h) of colchicine. The seedlings were then transferred to a culture medium for recovery and propagation. After clones were prepared from each seedling, octaploid clones were identified using flow cytometry. Chromosome counting was also used to confirm flow cytometric results in tetraploid (2n = 4x = 60; 2C DNA= 1.90 ± 0.01 pg) and octaploid (2n = 8x = 120; 2C DNA= 3.82 ± 02 pg) plants. The highest polyploidy induction efficiency with 32% was related to 0.05 % colchicine and 12 h duration. The results showed that the phenotypic traits of anatomical (stomata size, leaf guard cell size), morphological (stem diameter, length, width, and leaf area), physiological (soluble sugars, phenols, and flavonoids), and phytochemical (essential oil yield, P-cymene, γ-Terpinene, α-Thujene, and α-Pinene) properties significantly increased in octaploid plants, while the density of leaf stomata decreased compared to tetraploid plants. Our results verified that octaploid induction in Satureja mutica is an effective breeding method, remarkably increasing the quantitative and qualitative characteristics, which could be used as a new genetic resource in future breeding programs.


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Adams RP. 2007. Identification of essential oil components by gas chromatography/ mass spectrometry. 4th Edition. Allured Publ. Carol Stream

Aqafarini A, Lotfi M, Norouzi M, Karimzadeh G. 2019. Induction of tetraploidy in garden cress : morphological and cytological changes. Plant Cell Tissue Organ Cult 137, 627–635. https://doi.org/10.1007/s11240-019-01596-5

Chen L, Gao S (2007) In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus. Sci Hortic 112(3): 339-344. https://doi.org/10.1016/j.scienta.2006.12.045

ChuanHua D, Ying L, LinXuan L, JinYuan C, Ying L, ZhenDe Y, KunHua W (2019) Comprehensive evaluation of tetraploid strains of Sophora tonkinensis Gagnep. J South Agric 50(11): 2401–2409. https://doi.org/10.3969/j.issn.2095-1191.2019.11.04

Chung HH, Shi SK, Huang B, Chen JT (2017) Enhanced agronomic traits and medicinal constituents of autotetraploids in Anoectochilus formosanus Hayata, a top-grade medicinal orchid. Molecules 22: 1–13. https://doi.org/10.3390/molecules22111907

Dahanayake N, Chen XL, Zhao FC, Yang YS, Wu H (2011) Separation of trtraploid and diploid plants from chimeras in in vitro cultures of purple coneflower (Echi nacea purpurea L). Trop Agr Res Ext 13(1): 11-15. https://doi.org/10.4038/tare.v13i1.3131

Gantait S, Mandal N, Bhattacharyya S, Das PK (2011) Induction and identification of tetrapolids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tissue Organ Cult 106: 485–493. https://doi.org/10.1007/s11240-011-9947-1

Hadian J, Kavand S, Ahmadi J, Khadivi-Khub A (2014) Genotypic diversity and structure of Satureja mutica revealed by intersimple sequence repeat markers. Biochem Syst Ecol 54: 48–52. https://doi.org/10.1016/j.bse.2013.12.024

Hannweg K, Visser G, de Jager K, Bertling I (2016). In vitro-induced polyploidy and its effect on horticultural characteristics, essential oil composition and bioactivity of Tetradenia riparia. S Afr J Bot 106: 186–191. https://doi.org/10.1016/j.sajb.2016.07.013

Javadian N, Karimzadeh G, Sharifi M, Moieni A, Behmanesh M (2017) In vitro polyploidy induction: changes in morphology, podophyllotoxin biosynthesis, and expression of the related genes in Linum album (Linaceae). Planta https://doi.org/10.1007/s00425-017-2671-2

Julião SA, Ribeiro C do V, Lopes JML, Matos EM de, Reis AC, Peixoto PHP, … Viccini LF (2020) Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba. Front Plant Sci 11: 1–11. https://doi.org/10.3389/fpls.2020.00292

Kamtekar S, Keer V, Patil V (2014) Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. J. Appl. Pharm. Sci 4(9): 61–65. https://doi.org/10.7324/JAPS.2014.40911

Karimzadeh G, Danesh-Gilevaei M, Aghaalikhani M (2011) Karyotypic and nuclear DNA variations in Lathyrus sativus (Fabaceae). Caryologia 64: 42–54. https://doi.org/10.1080/00087114.2011.10589763

Kasmiyati S, Kristiani EBE, Herawati, MM (2020) Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina. Biosaintifika: Int J Biol Educ 12(1): 90–96. https://doi.org/10.15294/biosaintifika.v12i1.22548

Kumar De KK, Saha A, Tamang R, Sharma B. (2010) Investigation on relative genome sizes and ploidy levels of Darjeeling-Himalayan Rhododendron species using flow cytometer. Indian J Biotechnol 9(1): 64–68. https://www.researchgate.net/publication/267416834

Li M, Ding B, Huang W, Pan J, Ding Z, Jiang F (2018) Induction and Characterization of Tetraploids from Seeds of Bletilla striata (Thunb.) Reichb.f. Biomed Res Int 3246398. https://doi.org/10.1155/2018/3246398

Mahdavi S, Karimzadeh G (2010) Karyological and nuclear DNA content variation in some Iranian endemic Thymus species (Lamiaceae). J Agric Sci Technol 12: 447–458.

Majdi M, Karimzadeh G (2010) Induction of Tetraploidy to Feverfew (Tanacetum parthenium Physiological, Cytological, and Phytochemical Changes. HortScience 45(1): 16–21. https://doi.org/10.21273/hortsci.45.1.16

Manzoor A, Ahmad T, Bashir MA, Baig MMQ, Quresh AA, Shah MKN, Hafiz IA (2018) Induction and identification of colchicine induced polyploidy in Gladiolus grandiflorus “White Prosperity.” Folia Hortic 30(2): 307–319. https://doi.org/10.2478/fhort-2018-0026

Mathura S, Fossey A, Beck S (2006) Comparative study of chlorophyll content in diploid and tetraploid black Wattle (Acacia mearnsii). Forestry 79(4): 381–388. https://doi.org/10.1093/forestry/cpl023

Mei B, Xie H, Xing H, Kong D, Pan X, Li Y, Wu H (2020) Changes of Phenolic Acids and Antioxidant Activities in Diploid and Tetraploid Echinacea purpurea at Different Growth Stages. Rev Bras Farmacogn 30(4): 510–518. https://doi.org/10.1007/s43450-020-00069-7

Mo L, Chen J, Lou X, Xu X, Dong R, Tong Z, Huang H, Lin E (2020) Colchicine-induced polyploidy in Rhododendron fortunei Lindl. Plants 9(424): 1–13. https://doi.org/10.3390/plants9040424

Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Niazian M, Nalousi AM (2020) Artificial polyploidy induction for improvement of ornamental and medicinal plants. Plant Cell Tiss Organ Cult 142: 447–469. https://doi.org/10.1007/s11240-020-01888-1

Nosrati H (2014) Relationship between ploidy level and genome size in strawberries. Plant Biosyst 149(6): 1036–1041. https://doi.org/10.1080/11263504.2014.941036

Pandit MK, White SM, Pocock MJO (2014) The contrasting effects of genome size, chromosome number and ploidy level on plant invasiveness: A global analysis. New Phytol 203(2): 697–703. https://doi.org/10.1111/nph.12799

Parida, B.P. & Mishra, B.B. (2015). Is a plants ploidy status reflected in its metabolome?. Postdoc J 3(4): 1-11. https://doi.org/10.14304/surya.jpr.v3n4.1

Pharmacopée européenne (1997) 3rd Edn, Maisonneuve, Sante Ruffine.

Rao S, Tian Y, Xia X. Li Y, Chen J (2020) Chromosome doubling mediates superior drought tolerance in Lycium ruthenicum via abscisic acid signaling. Hortic Res 7(40):1-18. https://doi.org/10.1038/s41438-020-0260-1

Roy AT, Leggett G, Koutoulis A (2001) In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L). Plant Cell Rep 20(6): 489-495. https://doi.org/10.1007/s002990100364

Salma U, Kundu S, Mandal N (2017) Artificial polyploidy in medicinal plants: Advancement in the last two decades and impending prospects. J Crop Sci Biotechnol 20: 9-19. https://doi.org/10.1007/s12892-016-0080-1

Sattler MC, Carvalho CR, Clarindo WR (2016) The polyploidy and its key role in plant breeding. Planta 243: 281–296. https://doi.org/10.1007/s00425-015-2450-x

Sefidkon F, Jamzad Z (2004) Essential oil composition of Satureja spicigera ( C Koch) Boiss from Iran. Flavour Fragr J 19: 571–573. https://doi.org/10.1002/ffj.1357

Shao J, Chen C, Deng X (2003) In vitro induction of tetraploidy in pomegranate (Punica granatum). Plant Cell Tiss Organ Cult 75(3): 241-246. https://doi.org/10.1023/a:1025871810813

Shariat A, Karimzadeh G, Assareh MH (2013) Karyology of Iranian Endemic Satureja (Lamiaceae) Species. Cytologia, 78(3): 305–312. https://doi.org/10.1508/cytologia.78.305

Shariat A, Karimzadeh G, Assareh MH, Esfahan EZ (2016) Drought Stress in Iranian Endemic Savory (Satureja rechingeri ): In vivo and In vitro Studies. Plant Physiol Breed 6(1): 1–13.

Shariat A, Karimzadeh G, Assareh MH, Loureiro J (2018a) Relationships between genome size , morphological and ecological traits in Satureja ( Lamiaceae) species. Iran J Bot 24(2): 163–173. https://doi.org/10.22092/ijb.2018.122705.1211

Shariat A, Karimzadeh G, Assareh MH, Hadian J (2018b) Metabolite profiling and molecular responses in a drought-tolerant savory, Satureja rechingeri exposed to water deficit. 3 Biotech 8(11): 1-11. https://doi.org/10.1007/s13205-018-1491-9

Smith S, Weyers JDB, Berry WG (1989) Variation in stomatal characteristics over the lower surface of Commelina communis leaves. Plant Cell Environ 12: 653–659. https://doi.org/10.1111/j.1365-3040.1989.tb01234.x

Tarkesh Esfahani ST, Karimzadeh G, Naghavi MR (2020) In vitro polyploidy induction in persian poppy (Papaver bracteatum lindl.). Caryologia, 73(1): 133–144. https://doi.org/10.13128/caryologia-169

Tavan M, Mirjalili MH, Karimzadeh G (2015). In vitro polyploidy induction: changes in morphological, anatomical and phytochemical characteristics of Thymus persicus (Lamiaceae). Plant Cell Tissue Organ Cult 122(3): 573–583. https://doi.org/10.1007/s11240-015-0789-0

Touchell DH, Palmer IE, Ranney TG (2020) In vitro Ploidy Manipulation for Crop Improvement. Front Plant Sci 11: 1–11. https://doi.org/10.3389/fpls.2020.00722

Wei KH, Xu JP, Li LX, Cai JY, Miao JH, Li MH (2018) In vitro Induction and Generation of Tetraploid Plants of Sophora tonkinensis Gapnep. Pharmacogn Mag 14(54): 149–154. https://doi.org/10.4103/pm.pm

Wu J, Sang Y, Zhou Q , Zhang P (2020) Colchicine in vitro tetraploid induction of Populus hopeiensis from leaf blades. Plant Cell Tiss Organ Cult 141: 339–349. https://doi.org/10.1007/s11240-020-01790-w




How to Cite

Shariat, A., & Sefidkonb, F. (2022). Enhanced morphologic traits and medicinal constituents of octaploids in Satureja mutica, a high-yielding medicinal savory. Caryologia, 75(1), 41-53. https://doi.org/10.36253/caryologia-1264