Repetitive DNA mapping on Oligosarcus acutirostris (Teleostei, Characidae) from the Paraíba do Sul River Basin in southeastern Brazil

Authors

  • Marina Souza Cunha Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais http://orcid.org/0000-0003-3512-791X
  • Silvana Melo Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais
  • Filipe Schitini Salgado Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais
  • Cidimar Estevam Assis Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais
  • Jorge Abdala Dergam Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais http://orcid.org/0000-0003-1395-1377

DOI:

https://doi.org/10.36253/caryologia-1270

Keywords:

Characiformes, cytotaxonomy, coastal river basins, fluorescence in situ Hybridization (FISH), freshwater fishes

Abstract

Within the Neotropical region, the genus Oligosarcus represents an interesting assembly of small-sized freshwater predators. The goal of this study was to cytogenetically analyze Oligosarcus acutirostris from the Espírito Santo Stream, Paraíba do Sul River Basin. The following cytogenetic techniques were performed: Giemsa staining, Ag-NOR and C- bandings, Fluorescence in situ Hybridization (FISH) using 18S and 5S rDNA probes, and (CA)15 and (GA)15 microsatellite probes. Diploid number was 2n=50 and the karyotypic formula 4m+14sm+18st+14a. Ag-NOR sites were present on the subtelocentric chromosome pair number 10. C-banding showed a few pericentromeric and conspicuous terminal heterochromatic blocks. The 18S and 5S rDNA probes marked chromosome pairs number 10 and number 19, respectively. FISH patterns obtained with (CA)15 and (GA)15 probes hybridized pericentromeric and terminal regions in almost all chromosomes, and interstitial regions of some chromosomes. Interestingly, microsatellite (CA)15 showed a conspicuous centromeric mark on chromosome pair number 14, which could be an autapomorphy of this species, or it might characterize some species of  this genus. The Oligosarcus cytogenetic patterns suggest that this genus is prone to fixation of chromosomal rearrangements and may be useful to detect biogeographical subunits within the coastal Brazilian basins.

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Published

2022-03-08

How to Cite

Cunha, M. S., Melo, S., Salgado, F., Assis, C., & Dergam, J. (2022). Repetitive DNA mapping on Oligosarcus acutirostris (Teleostei, Characidae) from the Paraíba do Sul River Basin in southeastern Brazil. Caryologia, 74(4), 121-128. https://doi.org/10.36253/caryologia-1270

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