Segregation of the univalent X chromosome in the wide-footed treehopper Enchenopa latipes (Say 1824)

Authors

  • Kristen D. Felt Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Makayla B. Lagerman Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Samantha Maurer Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Lu Qian Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Oluwasefunmi Oluwafemi Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Noemi Pedraza-Aguado Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Emily L. Stowe Biology Department, Bucknell University, Lewisburg, Pennsylvania
  • Leocadia V. Paliulis Biology Department, Bucknell University, Lewisburg, Pennsylvania https://orcid.org/0000-0002-8244-7548

DOI:

https://doi.org/10.36253/caryologia-1411

Keywords:

chromosomes, bivalent, univalent, X chromosome, meiosis, Auchenorrhynca, Enchenopa latipes

Abstract

In metaphase I, autosomal bivalents align on the metaphase plate, while naturally-occurring univalent sex chromosomes can display a number of different behaviours depending on cellular conditions. Here we describe the behaviour of the univalent X chromosome in the wide-footed treehopper Enchenopa latipes (Say 1824). We confirm the chromosome number and sex determination method for this species, and that males possess a univalent X chromosome. We show that the univalent X chromosome forms a bipolar attachment to the spindle in metaphase I, and then segregates intact toward one spindle pole in late anaphase I (long after autosomes have initiated poleward movement). Movement of the univalent toward one pole is associated with loss of microtubule connections toward the opposite spindle pole.

Downloads

Download data is not yet available.

References

Ault JG. 1984. Unipolar orientation stability of the sex univalent in the grasshopper (Melanoplus sanguinipes). Chromosoma. 89:201-205.

Bauer H, Dietz R, and Röbbelen C. 1961. Die spermatocytenteilungen der tipuliden. III. das bewegungsverhalten der chromsomen in translokationheterozygoten von Tipula oleracea. Chromosoma. 12:116-189.

Bhattacharya AK, Manna GK. 1973. Morphology, behaviour, and metrical studies of the germinal chromosomes of ten species of Membracidae (Homoptera). Cytologia. 38:657-665.

Boring AM. 1907. A study of the spermatogenesis in twenty-two species of the Membracidae, Jassidae, Cercopidae, and Fulgoridae [dissertation]. Bryn Mawr (PA): Bryn Mawr College.

Boring AM. 1913. The chromosomes of the Cercopidae. Biol. Bull. 24:133-146.

Bressa MJ, Papeschi AG, Mola L, Larramendy M L. 2001. Autosomal univalents as a common meiotic feature in Jadera haematoloma (Herrich-Schaeffer, 1847) and Jadera sanguinolenta (Fabricius, 1775) (Heteroptera: Rhopalidae: Serinethinae). Eur. Jour. Ent. 98:151-157.

Dietrich C H, McKamey S H, Deitz LL. 2001. Morphology-based phylogeny of the treehopper family Membracidae (Hemiptera: Cicadomorpha: Membracoidea). Systematic Entomology 26:213-239.

Dietz R. 1954. Multiple geschlechtschromosomen bei dem ostracoden Notodromas monacha. Chromosoma. 6:397-418.

Dietz R. 1969. Bau und funktion des spindelapparats. Naturwissenschaften, 56:237-248.

Fabig G, Kiewisz R, Lindow N, Powers JA, Cota V, Quintanilla LJ, et al. 2020. Male meiotic spindle features that efficiently segregate paired and lagging chromosomes. eLife. 10:9:e50988.

Felt KD, Lagerman M, Ravida N, Qian L, Powers S, Paliulis LV. 2017. Segregation of the amphitelically-attached univalent X chromosome in the spittlebug Philaenus spumarius. Protoplasma. 254:2263-2271.

Fabig G, Müller-Reichert T, Paliulis LV. 2016. Back to the roots: Segregation of univalent sex chromosomes in meiosis. Chromosoma. 125:277-286.

Halkka O. 1959. Chromosome studies on the Hemiptera Homoptera Auchenorrhyncha. Ann. Acad. Sci. Fenn. A. IV. 43:1-72.

Halkka O. 1962. The chromosomes of the Membracidae. Hereditas. 48:215-219.

Halkka O, Heinonen L. 1964. The chromosomes of 17 nearctic homoptera. Hereditas. 52:77-80.

John B. 1990. Meiosis. Cambridge (UK): Cambridge University Press.

John B, Claridge MF. 1974. Chromosome variation in British populations of Oncopsis (Hemiptera: Cicadellidae). Chromosoma. 46:77-89.

Kopp DD, Yonke TR. 1973. The treehoppers of Missouri: Part 1. Subfamilies Centrotinae, Hoplophorioninae, and Membracinae (Homoptera: Membracidae). Journal of the Kansas Entomological Society. 46:42-64.

Kuznetsova V, Aguin-Pombo D. 2015. Comparative cytogenetics of Auchenorrhyncha (Hemiptera, Homoptera): a review. Zookeys. 19:63-93.

Lin K, Nance R, Szybist J, Cheville A, Paliulis LV. 2018. Micromanipulation of insect spermatocytes. Journal of Visualized Experiments. 140:57359.

Madeira F, Park YM, Lee J, Buso N, Gur T, Madhusoodanan N, Basutkar P, Tivey ARN, Potter SC, Finn RD, Lopez R. 2019. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 47:W636-W641.

Melters DP, Paliulis LV, Korf IF, Chan SWL. 2012. Holocentric chromosomes: Convergent evolution, meiotic adaptations, and genomic analysis. Chromosome Res. 20:579-593.

Moore DP, Orr-Weaver TL. 1998. Chromosome segregation during meiosis: building an unambivalent bivalent. Curr Top Dev Biol. 37:263–299.

Nokkala S. 1986. The mechanisms behind the regular segregation of autosomal univalents in Calocoris quadripunctatus (vil.) (Miridae, Hemiptera). Hereditas. 105:199-204.

Rao SRV. 1956. Studies on the spermatogenesis of some Indian Homoptera Part III: A study of chromosomes in two members of the family Membracidae. Caryologia, 8:309-315.

Rebollo E, Arana P. 1995. A comparative study of orientation at behavior of univalent in living grasshopper spermatocytes. Chromosoma. 104:56-67.

Rebollo E, Martín S, Manzanero S, Arana P. 1998. Chromosomal strategies for adaptation to univalency. Chromosome Res. 6:515-531.

Rebollo E, Arana P. 1997. Univalent orientation in living meiocytes. Chromosomes Today. 12:249-269.

Rebollo E, Arana P. 1998. Chromosomal factors affecting the transmission of univalents. Chromosome Res. 6:67-69.

Tian R, Yuan F. 1997. Chromosomes in twenty-five species of Chinese Membracids (Homoptera: Membracidae). Insect Science. 4:150-158.

Downloads

Published

2022-07-07

How to Cite

Felt, K. D., Lagerman, M. B., Maurer, S., Qian, L., Oluwafemi, O., Pedraza-Aguado, N., Stowe, E. L., & Paliulis, L. V. (2022). Segregation of the univalent X chromosome in the wide-footed treehopper Enchenopa latipes (Say 1824). Caryologia, 75(1), 165-171. https://doi.org/10.36253/caryologia-1411

Issue

Section

Articles