Nuclear DNA 2C-values for 16 species from Timor-Leste increases taxonomical representation in tropical ferns and lycophytes

Authors

DOI:

https://doi.org/10.36253/caryologia-1628

Keywords:

genome size, chromosome, cytogenetics, DNA amount, nuclear DNA content, Malesia, geographical distribution

Abstract

Knowledge regarding genome size allows us to infer relationships between taxa, address questions related to systematics and contribute to biodiversity studies. However, currently, less than 3% of the described Pteridophyta species have genome size estimates reported in databases, and only around one third of these are tropical species, although the tropics are home of 86% of fern diversity. The region of Timor-Leste, included in one of the 25 hotspots of biodiversity, is considered one of the richest areas of the world in terms of pteridophyte species. Nonetheless, biodiversity-driven research focused on this territory’s biodiversity is scarce. This study presents novel 2C-values for 15 species of ferns collected in Timor-Leste, using flow cytometry. Furthermore, one species of the lycophyte Palhinhaea cernua (L.) Vasc. & Franco, was also studied and its estimated genome size compared to a previous report. Estimates ranged from 10.45 pg in Selliguea feei Bory to 29.7 pg in Microsorum punctatum (L.) Copel, and are considered medium-size genomes. The data was compared with previous reports for closely related species. These are the first 2C-values for two families and seven genera of ferns, increasing the number of pteridophytes with reported C-values from 292 to 307.

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References

Antonelli A, Fry C, Smith RJ, Simmonds MSJ, Kersey PJ, Pritchard HW, Abbo MS, Acedo C, Adams J, Ainsworth AM, Allkin B, et al. 2020. State of the world’s plants and fungi 2020. Royal Botanic Gardens, Kew. DOI: https://doi.org/10.34885/172

Bainard J, Henry, TA, Bainard LD, Newmaster SG. 2011. DNA content variation in monilophytes and lycophytes: large genomes that are not endopolyploid. Chromosome Res. 19(6): 763-775. DOI: https://doi.org/10.1007/s10577-011-9228-1

Bennett M, Leitch IJ. 1995. Nuclear DNA Amounts in angiosperms. Ann Bot. 76(2), 113–176. DOI: https://doi.org/10.1006/anbo.1995.1085

Bennett M, Leitch IJ. 2001. Nuclear DNA amounts in pteridophytes. Ann Bot. 87(3): 335-345. DOI : https://doi.org/10.1006/anbo.2000.1339

Bouma GA, Kobryn HT. 2004. Change in vegetation cover in East Timor, 1989-1999. Nat Resour Forum. 28(1): 1-12. DOI: https://doi.org/10.1111/j.0165-0203.2004.00067.x

Clark J, Hidalgo O, Pellicer J, Liu H, Marquardt J, Robert Y, Christenhusz M, Zhang S, Gibby M, Leitch IJ, Schneider H. 2016. Genome evolution of ferns: evidence for relative stasis of genome size across the fern phylogeny. New Phytol. 210(3): 1072-1082. DOI: https://doi.org/10.1111/nph.13833Dagher-Kharrat MB, Abdel-Samad N, Douaihy B, Bourge M, Fridlender A, Siljak-Yakovlev S, Brown SC. 2013. Nuclear DNA C-values for biodiversity screening: Case of the Lebanese flora. Plant Biosyst. 147(4): 1228-1237. DOI: https://doi.org/10.1080/11263504.2013.861530

Dolezel J. 2005. Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot. 95(1): 99–110. DOI: https://doi.org/10.1093/aob/mci005

Dolezel J, Sgorbati S, Lucretii S. 1992. Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiol Plant. 85(4): 625-631. DOI: https://doi.org/10.1111/j.1399-3054.1992.tb04764.x

Ebihara A, Kuo LY. 2012. East and Southeast Asian pteridophyte flora and DNA barcoding. In: Nakano S., Yahara T., Nakashizuka T. (eds) The Biodiversity Observation Network in the Asia-Pacific Region. Ecological Research Monographs. Tokyo (TK): Springer. DOI: https://doi.org/10.1007/978-4-431-54032-8_2

Galbraith DW. 2009. Simultaneous flow cytometry quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values. Cytometry A. 75A(8): 692-698. DOI: https://doi.org/10.1002/cyto.a.20760

Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science. 220(4601): 1049–1051. DOI: https://doi.org/10.1126/science.220.4601.1049

Hanusová K, Ekrt L, Vít P, Kolár F, Urfus T. 2014. Continuous morphological variation correlated with genome size indicates frequent introgressive hybridization among Diphasiastrum species (lycopodiaceae) in central Europe. Plos One. 9(6): e99552 DOI: https://doi.org/10.1371/journal.pone.0099552

Knight CA, Molinari NA, Petrov DA. 2005. The large genome constraint hypothesis: evolution, ecology and phenotype. Ann Bot. 95(1): 177-190. DOI: https://doi.org/10.1093/aob/mci011

Kumar PP, Turner IM, Nagaraja R, Arumuganathan K. 2011. Estimation of nuclear DNA content of various bamboo and rattan species. Plant Biotechnol Rep. 5(3): 317–322. https://doi.org/10.1007/s11816-011-0185-0

Leitch IJ. 2005. Evolution of DNA amounts across land plants (Embryophyta). Ann Bot. 95(1): 207–217. DOI: https://doi.org/10.1093/aob/mci014

Leitch IJ, Chase MW, Bennet MD. 1998. Phylogenetic analysis of DNA C-values provides evidence for a small ancestral genome size in flowering plants. Ann Bot. 82(Supplement A): 85-94. DOI: https://doi.org/10.1006/anbo.1998.0783

Leitch IJ, Johnston E, Pellicer J, Hidalgo O, Bennett MD. 2019. Pteridophyte DNA C-values database (release 7.1, Apr 2019) https://cvalues.science.kew.org/

Liu H, Ekrt L, Koutecky P, Pellicer J, Hidalgo O, Marquardt J, Pustahija F, Ebihara A, Siljak-Yakovlev S, Gibby M, Leitch I, Schneider H. 2019. Polyploidy does not control all: Lineage-specific average chromosome length constraints genome size evolution in ferns. J Syst Evol. 57(4): 418-430. DOI: https://doi.org/doi.org/10.1111/jse.12525

Loureiro J, Rodriguez E, Dolezel J, Santos C. 2007. Two new nuclear isolation buffers for plant DNA flow cytometry—a test with 37 species. Ann Bot. 100:875–888.

Moran RC. 2008. Diversity, biogeography, and floristics. In T. A. Ranker & C. H. Haufler (Eds.) Biology and Evolution of Ferns and Lycophytes. New York (NY): Cambridge, 367-394.

Myers N, Mittermeier R, Mittermeier C, da Fonseca GAB, Kent J. 2002. Biodiversity hotspots for conservation priorities. Nature. 403(6772): 853–858. DOI: https://doi.org/doi.org/10.1038/35002501

Obermayer R, Leitch IJ, Hanson L, Bennet MD. 2002. Nuclear DNA C-values in 30 species double the familial representation in pteridophytes. Ann Bot. 90(2): 209-217. DOI: https://doi.org/10.1093/aob/mcf167

Ohri D. 1998. Genome size variation and plant systematics. Ann Bot. 82(Supplement 1), 75–83. DOI:10.1006/anbo.1998.0765

Pellicer J, Leitch IJ. 2013. The application of flow cytometry for estimating genome size and ploidy level in plants. Molecular Plant Taxonomy. 1115: 279–307. DOI: https://doi.org/10.1007/978-1-62703-767-9_14

Plant DNA C-value database. 2001-. Release 7.1. Royal Botanic Gardens, Kew (UK): [updated 2019 April; accessed 2021 Oct ] https://cvalues.science.kew.org/)

[PPG] Pteridophyte Phylogeny Group. 2016. A community-derived classification for extant lycophytes and ferns. J Syst Evol. 54(6): 563–603. DOI: https://doi.org/10.1111/jse.12229.

Rice A, Glick L, Abad S, Einhorn M, Kopelman NM, Salman-Minkov A, Mayzel J, Chay O, Mayrose I. 2015. The Chromosome Counts Database (CCDB) – a community resource of plant chromosome numbers. New Phytol. 206(1): 19-26.

Takhtajan A. 1986. Floristic regions of the world. Berkley and Los Angeles: University of California Press (CA)

Wagner FS. 1992. Cytological problems in Lycopodium Sens. Lat. Ann Mo Bot Gard. 79(3): 718-729. DOI: https://doi.org/10.2307/2399761

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Published

2023-02-12

How to Cite

Simão, I., Costa, H., Oliveira, H., Silva, H., & Silveira, P. (2023). Nuclear DNA 2C-values for 16 species from Timor-Leste increases taxonomical representation in tropical ferns and lycophytes. Caryologia, 75(3), 109–122. https://doi.org/10.36253/caryologia-1628

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Vol. 75 No. 3 (2022)

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Copyright (c) 2022 Paulo Silveira, Inês Simão, Hermenegildo Costa, Helena Oliveira, Helena Silva

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