L-Ascorbic acid modulates the cytotoxic and genotoxic effects of salinity in barley meristem cells by regulating mitotic activity and chromosomal aberrations
Keywords:cytotoxicity, genotoxicity, Hordeum vulgare L., mitotic index, ascorbic acid, salinity
The objective of the present study was to with all details explain of the efficiency of L-ascorbic acid (L-AsA) also known as vitamin C on cytotoxicity and genotoxicity induced by salt stress in the barley apical meristems. As a result of the statistical analysis salt stress caused a significant (P ≤ 0.05) decrease in mitotic index of barley seeds depending on concentration increase, while the frequency of chromosomal aberration (CA) increased. In addition, it was determined that mitotic index value was decreased by 46% with 1 μM L-AsA supplementation as compared to control and chromosomal abnormalities were increased by 8.96% as well as. However, in the case of simultaneously application of 1 μM L-AsA and different salt concentrations, the high salt concentrations exhibited an excellent success according to low salt concentrations in alleviating the mitodepressive effect of salt stress. Moreover, the frequency of chromosomal aberrations in the root meristem cells of those seeds with 1 μM L-AsA supplementation germinated at different salt concentrations was substantially reduced compared to own control group (alone 1 μM L-AsA pretreatment). The 1 μM L-AsA pretreatment at the highest salt concentration (at 0.40 M) was showed an excellent success by reducing the frequency of the chromosomal aberrations by approximately 90 %. Different salt concentrations and/or 1 μM L-AsA supplementation caused micronuclei and granulation as well as various chromosomal aberrations in prophase, metaphase, anaphase and telophase.
Akram NA, Shafiq F, Ashraf M. 2017. Ascorbic acid- a potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Front Plant Sci. 8:613.
Asensi-Fabado MA, Amtmann A, Perrella G. 2017. Plant responses to abiotic stress: The chromatin context of transcriptional regulation. Biochim Biophys Acta. 1860:106-122.
Ashraf M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv. 27:84-93.
Asita AO, Mokhobo MM. 2013. Clastogenic and cytotoxic effects of four pesticides used to control insect pests of stored products on root meristems of Allium cepa. Environ Nat Resour Res. 3(2):133–145.
Asita AO, Moramang S, Rant?o T, Magama S. 2017. Modulation of mutagen-induced genotoxicity by vitamin C and medicinal plants in Allium cepa L. Caryologia. 70:151-165.
Athar HR, Khan A, Ashraf M. 2008. Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environ Exp Bot. 63:224-231.
Athar HR, Khan A, Ashraf M. 2009. Inducing salt tolerance in wheat by exogenously applied ascorbic acid through different modes. J Plant Nutr. 32:1799-1817.
Barakat H. 2003. Interactive effects of salinity and certain vitamins on gene expression and cell division. Int J Agric Biol. 3:219-225.
Bargaz A, Nassar RMA, Rady MM, Gaballah MS, Thompson SM, Brestic M, Schmidhalter U, Abdelhamid MT. 2016. Improved salinity tolerance by phosphorus fertilizer in two Phaseolus vulgaris recombinant inbred lines contrasting in their P-efficiency. J Agron Crop Sci. 202:497-507.
Bassuony FM, Hassanein RA, Baraka DM, Khalil RR. 2008. Physiological effects of nicotinamide and ascorbic acid on Zea mays plant grown under salinity stress II-changes in nitrogen constituents, protein profiles, protease enzyme and certain inorganic cations. Aust J Basic & Appl Sci. 2(3):350-359.
Bonciu E, Firbas P, Fontanetti CS, Wusheng J, Karaismailo?lu MC, Liu D, Menicucci F, Pesnya DS, Popescu A, Romanovsky AV, Schiff S, ?lusarczyk J, Souza CP, Srivastava A, Sutan A, Papini A. 2018. An evaluation for the standardization of the Allium cepa test as cytotoxicity and genotoxicity assay. Caryologia. 71(3):191-209.
Briand CH, Kapoor BM. 1989 The cytogenetic effects of sodium salicylate on the root meristem cells of Allium sativum L. Cytologia 54:203–209.
Cenanovic M, Durakovic C. 2016. In vivo genotoxicity testing of vitamin C and naproxen sodium using plant bioassay. Southeast Europe J Soft Comput 4(2):66-71.
Chen X, Zhou Y, Cong Y, Zhu P, Xing J, Cui J, Xu W, Shi Q, Diao M, Liu HY. 2021. Ascorbic acid-induced photosynthetic adaptability of processing tomatoes to salt stress probed by fast OJIP fluorescence rise. Front Plant Sci. 12:594400.
Çavu?o?lu K, Bilir G. 2015. Effects of ascorbic acid on the seed germination, seedling growth and leaf anatomy of barley under salt stress. ARPN J Agric Biol Sci. 10(4):124-129.
Çavu?o?lu D, Tabur S, Çavu?o?lu K. 2016a. The effects of Aloe vera L. leaf extract on some physiological and cytogenetical parameters in Allium cepa L. seeds germinated under salt stress. Cytologia. 81(1):103-110.
Çavu?o?lu D, Tabur S, Çavu?o?lu K. 2016b. Role of Ginkgo biloba L. leaf extract on some physiological and cytogenetical parameters in Allium cepa L. seeds exposed to salt stress. Cytologia. 81(2):207-213.
Dane F, Dalg?ç Ö. 2005. The Effect of fungicide benomyl (benlate) on growth and mitosis in onion (Allium cepa L.) root apical meristem. Acta Biol Hung. 56 (1-2):119-128.
Dizlek H, Gül H. 2007. L-ascorbic acid and its functions at bread making. Süleyman Demirel Univ J Fac Agric. 2(1):26-34.
Dolatabadian A, Jouneghani RS. 2009. Impact of exogenous ascorbic acid on antioxidant activity and some physiological traits of common bean subjected to salinity stress. Not Bot Horti Agrobot Cluj-Napoca. 37(2):165-172.
Duncan DB. 1955. Multiple range and multiple F tests. Biometrics 11:1-42.
El-Araby HG, El Hefnawy SFM, Nassar MA, Elsheery NI. 2020. Comparative studies between growth regulators and nanoparticles on growth and mitotic index of pea plants under salinity. Afr J Biotech 19(8):564-575.
El-Bassiouny MSH, Gobarah ME, Ramadan AA. 2005. Effect of antioxidants on growth, yield and favism causative agents in seeds of Vicia faba L. plants grown under reclaimed sandy soil. J Agron. 4:281-287.
Elçi ?, Sancak C. 2013. Research methods and observations in cytogenetics. Ankara Univ Publish House. Be?evler/ANKARA, 227p.
Elsheery NI, Helaly MN, Omar SA, John SVS, Zabochnicka-Swiatek M, Kalaji HM, Rastogi A. 2020a. Physiological and molecular mechanisms of salinity tolerance in grafted cucumber. South Afric J Bot. 130: 90-102.
Elsheery NI, Helaly MN, El-Hoseiny HM, Alam-Eldein SM. 2020b. Zinc oxide and silicone nanoparticles to improve the resistance mechanism and annual productivity of salt-stressed mango trees. Agronomy. 10:953-975.
Emam MM, Helal NM. 2008. Vitamins minimize the salt-induced oxidative stress hazards. Aust J Basic & Appl Sci. 2(4):1110- 1119
FAO 2016. FAOSTAT. Food and Agriculture Organization of the United Nations, Rome, Italy. Web. http://faostat.fao.org/default. aspx. Accessed: 12 February 2019.
Farheen J, Mansoor S, Abideen Z. 2018. Exogenously applied salicylic acid improved growth, photosynthetic pigments and oxidative stability in mungbean seedlings (Vigna radiata) at salt stress. Pak J Bot. 50:901-912.
Fatemi SN. 2014. Ascorbic acid and its effects on alleviation of salt stress in sunflower. Ann Res Rev Biol. 4(24):3656-3665.
Feretti D, Zerbini I, Zani C, Ceretti, Moretti M, Monarca S. 2007. Allium cepa chromosome aberration and micronucleus tests applied to study genotoxicity of extracts from pesticide-treated vegetables and grapes. Food Addit Contam. 24(6):561-572.
Fiskesjö G. 1985. The Allium test as a standard in environmental monitoring. Hereditas. 102:99-112.
Fiskesjö G. 1997. Allium test for screening chemicals; evaluation of cytological parameters. In: Wang W, Lower WR, Gorsuch JW, Hughes JS (eds) Plant for Environmental Studies. Lewis Publishers, New York, pp 308-333.
Fiskesjö G, Levan A. 1993. Evaluation of the first ten MEIC chemicals in the Allium test. Altern Lab Anim. 21:139–149.
Gaafar AA, Ali SI, El-Shawadfy A, Salama ZA, Sekara A, Ulrichs C, Abdelhamid MT. 2020. Ascorbic acid induces the increase of secondary metabolites, antioxidant activity, growth, and productivity of the common bean under water stress conditions. Plants. 9:627.
Gallie DR. 2013. Ascorbic acid: A multifunctional molecule supporting plant growth and development. Scientifca. 795964:1-24.
Hossain MA, Munne-Bosch S, Burritt DJ, Diaz-Vivancos P, Fujita M, Lorence A. 2017. Ascorbic acid in plant growth, development and stress tolerance. Springer, Cham 514p.
Khan MA, Ahmed MZ, Hameed A. 2006. Effect of sea salt and L-ascorbic acid on the seed germination of halophytes. J Arid Environ. 67(3):535-540.
Kie?kowska A. 2017. Cytogenetic effect of prolonged in vitro exposure of Allium cepa L. root meristem cells to salt stress. Cytol Genet. 51(6):478-484.
Kim JM, To TK, Nishioka T, Seki M. 2010. Chromatin regulation functions in plant abiotic stress responses. Plant Cell Environ. 33:604-611.
Klášterská I, Natarajan AT, Ramel C. 1976. An interpretation of the origin of subchromatid aberrations and chromosome stickiness as a category of chromatid aberrations. Hereditas. 83:153-162.
Kontek R, Osiecka R, Kontek B. 2007. Clastogenic and mitodepressive effects of the insecticide dichlorvos on root meristems of Vicia faba. J Appl Genet. 48(5):359-361.
Lutsenko EK, Marushko EA, Kononenko NV, Leonova TG. 2005. Effects of fusicoccin on the early stages of sorghum growth at high NaCl concentrations. Russ J Plant Physiol. 52:332–337.
Mahfouz H, Rayan WA. 2017. Antimutagenics effects of stigmasterol on two salt stressed Lupinus termis cultivars. Egypt J Genet Cytol. 46:253-272.
Mesi A, Kopliku D. 2013. Cytotoxic and genotoxic potency screeningof two pesticides on Allium cepa L. Proc Tech. 8:19–26.
Mohsen AA, Ebrahim MKH, Ghoraba WFS. 2014. Role of ascorbic acid on germination indexes and enzyme activity of Vicia faba seeds grown under salinity stress. J Stress Physiol Biochem. 10(3):62-77.
Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annu Rev Plant Biol. 59:651-681.
Nag S, Dutta R, Pal KK. 2013. Chromosomal aberrations induced by acetamiprid in Allium cepa L. root meristem cells. Ind J Fund Appl Life Sci. 3(2):1-5.
Naser HM, El-Hosieny H, Elsheery NI, Kalaji HM. 2016. Effect of biofertilizers and putrescine amine on the physiological features and productivity of date palm (Phoenix dactylifera L.) grown on reclaimed salinized soil. Trees. 30:1149-1161.
Nassar R, Nermeen T, Reda F. 2016. Active yeast extract counteracts the harmful effects of salinity stress on the growth of leucaena plant. Scienta Hortic. 201:61-67.
Nunes LRL, Pinheiro PR, Silva JB, Dutra AS. 2020. Effects of ascorbic acid on the germination and vigour of cowpea seeds under water stress. Rev Ciênc Agron. 51(2):e20196629.
Özmen S, Tabur S. 2020. Functions of folic acid (vitamin B9) against cytotoxic effects of salt stress in Hordeum vulgare L. Pak J Bot. 52(1):17-22.
Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: A review. Ecotoxicol Environ Saf. 60:324-349.
Patil BC, Bhat GI. 1992. A comparative study of MH and EMS in the induction of chromosomal aberrations on lateral root meristem in Clitoria ternetea L. Cytologia. 57:259–264.
Pekol S, Balo?lu MC, Alt?no?lu YÇ. 2016. Evaluation of genotoxic and cytologic effects of environmental stress in wheat species with different ploidy levels. Turk J Biol. 40:580-588.
Rieder CL, Salmon ED. 1998. The vertebrate cell kinetochore and its roles during mitosis. Trends Cell Biol. 8:310–318.
Sabagh AE, Hossain A, Barutçular C, Islam MS, Ratnasekera D, Kumar N, Meena RS, Gharib HS, Saneoka H, Silva JAT. 2019. Drought and salinity stress management for higher and sustainable canola (Brassica napus L.) production: A critical review. Aust J Crop Sci. 13(1):88-97.
Shalata A, Neumann PM. 2001. Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. J Exp Bot. 364:2207- 2211.
Sharma PC, Gupta PK. 1982. Karyotypes in some pulse crops. Nucleus, 25: 181-185
Smirnoff N. 1996. The function and metabolism of ascorbic acid in plants. Ann Bot. 78(6):661-669.
Tabur S, Demir K. 2010a. Role of some growth regulators on cytogenetic activity of barley under salt stres. Plant Growth Regul. 60:99-104.
Tabur S, Demir K. 2010b. Protective roles of exogenous polyamines on chromosomal aberrations in Hordeum vulgare exposed to salinity. Biologia. 65:947-953.
Tabur S, Avc? ZD, Özmen S. 2021. Exogenous salicylic acid application against mitodepressive and clastogenic effects induced by salt stress in barley apical meristems. Biologia. 76:341–350.
Tobe K, Zhang L, Omasa K. 2003. Alleviatory effects of calcium on the toxicity of sodium, potassium and magnesium chlorides to seed germination in three nonhalophytes. Seed Sci Res. 13:47-54.
Türko?lu S. 2007. Genotoxicity of five food preservatives tested on root tips of Allium cepa L., Mut Res. 626:4-14.
Wang M, Ding F, Zhang S. 2020. Mutation of SlSBPASE aggravates chilling-induced oxidative stress by impairing glutathione biosynthesis and suppressing ascorbate-glutathione recycling in tomato plants. Front Plant Sci 11:565701.
Xie JQ, Li GX, Wang XK, Zheng QW, Feng ZZ. 2009. Effect of exogenous ascorbic acid on photosynthesis and growth of rice under O3 stress. Chin J Eco-Agric. 17:1176-1181.
Xu W. 2017. Alleviative effects and mechanism of exogenous ascorbic acid on chromium (Cr6+) toxicity in wheat. Nanjing: phd, Nanjing Agricultural Univ.
Vicente O, Boscaiu M, Naranjo MA, Estrelles E, Belle’s JM, Soriano P. 2004. Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). J Arid Environ. 58:463-481.
Yu CM, Xie FD, Ma LJ. 2014. Effects of exogenous application of ascorbic acid on genotoxicity of Pb in Vicia faba roots. Int J Agric Biol. 16(4):831-835.
Zhu M, Shabala S, Shabala L, Fan Y, Zhou MX. 2016. Evaluating predictive values of various physiological indices for salinity stress tolerance in wheat. J Agro Crop Sci. 202:115-124.
Zvanarou S, Vágnerová R, Mackievic V, Usnich S, Smolich I, Sokolik A, Yu M, Huang X, Angelis KJ, Demidchik V. 2020. Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema. Environ Exp Bot. 180:104236.
How to Cite
Copyright (c) 2022 Selma TABUR, Naime BÜYÜKKAYA-BAYRAKTAR, Serkan ÖZMEN
This work is licensed under a Creative Commons Attribution 4.0 International License.
- Copyright on any open access article in a journal published byCaryologia is retained by the author(s).
- Authors grant Caryologia a license to publish the article and identify itself as the original publisher.
- Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.
- The Creative Commons Attribution License 4.0 formalizes these and other terms and conditions of publishing articles.
- In accordance with our Open Data policy, the Creative Commons CC0 1.0 Public Domain Dedication waiver applies to all published data in Caryologia open access articles.