Karyotypic description and repetitive DNA chromosome mapping of Melipona interrupta Latreille, 1811 (Hymenoptera: Meliponini)
DOI:
https://doi.org/10.13128/cayologia-239Keywords:
Cytogenetics, Fluorescence in situ Hybridization (FISH), Heterochromatin, Melikerria, Stingless beeAbstract
Heterochromatic patterns in the genus Melipona vary among subgenera species. Melikerria is the only subgenus that presents species with different content of heterochromatin. Thus, the cytogenetic knowledge of other species of this subgenus is important for the understanding of karyotype evolution in Melipona. Here, we describe the karyotype of Melipona (Melikerria) interrupta based on the chromosomal heterochromatic patterns, AT and GC richness, mapping sequences of rDNA, microsatellites and telomeric regions. We observed 2n=18 chromosomes, with a high heterochromatin content rich in AT and euchromatic regions rich in GC base pairs. The high GC content was observed at interstitial region near the junction of the stained euchromatin and heterochromatin of the first chromosomal pair, the same region marked for the rDNA 18S locus. Microsatellites hybridized only on euchromatin regions and the telomeric probe on terminal regions of all chromosomes. Melipona (Melikerria) interrupta belongs to previous described heterochromatic Group II, suggesting there has been an increase in heterochromatin content in Melikerria. The M. quinquefasciata, belonging to the same subgenus as Melipona (Melikerria) interrupta, has low content of heterochromatin and appears to be evolving independently. So, the differences in the content heterochromatin, in the marker regions of CMA3 and the rDNA 18S locus in species of Melikerria is an important feature to be investigated further.
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References
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Ramírez SR, Nieh JC, Quental TB, Roubik DW, Imperatriz-Fonseca VL, Pierce NE. 2010. A molecular phylogeny of the stingless bee genus Melipona (Hymenoptera: Apidae). Mol. Phylogenet. Evol. 56 (2): 519-525.
Rasmussen C, Cameron AS. 2010. Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal. Biol. J. Linn. Soc. 99 (1): 206-232.
Rocha MP. 2002. Análises citogenéticas em abelhas do gênero Melipona Hymenoptera, Meliponini). Dissertation, Universidade Federal de Viçosa.
Rocha MP, Cruz MP, Fernandes A, Waldschmidt AM, Silva-Júnior JC, Pompolo SG. 2003. Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromossomes. Hereditas. 138 (2): 133-137.
Rocha MP, Pompolo SG. 1998. Karyotypes and heterochromatin variation (C-bands) in Melipona species (Hymenoptera, Apidae, Meliponinae). Genet. Mol. Biol. 21 (1): 41-45.
Rocha MP, Pompolo SG, Dergam JA, Fernandes A, Campos LAO. 2002. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). Hereditas. 136 (1): 19-27.
Rocha MP, Pompolo SG, Fernandes A, Campos LAO. 2007. Melipona: Six decade of cytogenetic. Biosc. J. 1: 111-117.
Sahara K, Marec F, Traut W. 1999. TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome Res. 7 (6): 449-460.
Schweizer D. 1980. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogen. Cell. Genet. 27 (6): 190-193.
Silva AA, Rocha MP, Pompolo SG, Campos LAO, Tavares MG. 2018. Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes. Comp. Cytogenet. 12 (4): 471.
Sumner AT. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res. 75 (1): 304-306.
Tavares MG, Lopes DM, Campos LAO. 2017. An overview of cytogenetics of the tribe Meliponine (Hymenoptera: Apidae). Genetica 145 (6): 1-18.
Barbosa ICO. 2018. Caracterização citogenética e análise do perfil de metilação em Melipona seminigra e Melipona interrupta (Hymenoptera, Apidae). Dissertation, Instituto Nacional de Pesquisas da Amazônia. 42pp.
Brito RM, Caixeiro APA, Pompolo SG, Azevedo GG. 2003. Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/DAPI. Genet. Mol. Biol. 26 (1): 53-57.
Camargo JMF, Pedro SRM. 2013. Meliponini Lepeletier, 1836. In Moure, JS, Urban, D. and Melo, GA R (Orgs). Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region - online version. Available at http://www.moure.cria.org.br/catalogue. Accessed 18 january 2019.
Cunha MS, Travenzoli NM, Ferreira RP, Cassinela EK, Silva H, Salomão TMF, Lopes DM. (2018). Comparative cytogenetics in three Melipona species (Hymenoptera: Apidae) with two divergent heterochromatic patterns. Genet. Mol. Biol. 41 (4): 806-813.
Francini IB, Gross MC, Nunes-Silva CG, Carvalho-Zilse GA. 2011. Cytogenetic analysis of the Amazon stingless bee Melipona seminigra merrillae reveals different chromosome number for the genus. Sci. Agric. 68 (5): 592-593.
Frydrychová R, Grossmann P, Trubac P, Vítková M, Marec FE. 2004. Phylogenetic distribution of TTAGG telomeric repeats in insects. Genome. 47: 163-178.
Godoy DC, Ferreira RP, Lopes DM. 2013. Chromosomal Variation and Cytogenetics of Plebeia lucii and P. phrynostoma (Hymenoptera: Apidae). Fla. Entomol. 96 (4): 1559-1566.
Imai HT, Taylor RW, Crosland MW, Crozier RH. 1988. Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis. JPN J. Genet. 63 (2): 159-185.
Kerr WE. 1969. Some aspects of the Evolution of social bees. Evol. Biol. 3, 119-175.
Kerr WE. 1972. Number of chromosomes in some species of bees. J. Kansas Entomol. Soc. 45: 111-122.
Lopes DM, Fernandes A, Praça-Fontes MM, Werneck HA, Resende HC, Campos LAO. 2011. Cytogenetics of three Melipona species (Hymenoptera, Apidae, Meliponini). Sociobiology. 57 (2): 185-194.
Lorite P, Carrillo JA, Palomeque T. 2002. Conservation of (TTAGG) n telomeric sequences among ants (Hymenoptera, Formicidae). J. Hered. 93: 282-285.
Menezes RS, Bardella, VB, Cabral-de-Mello DC, Lucena DA, Almeida EA. 2017. Are the TTAGG and TTAGGG telomeric repeats phylogenetically conserved in aculeate Hymenoptera?. Sci. Nat. 104 (9-10): 85.
Meyne J, Hirai H, Imai HT. 1995. FISH analysis of the telomere sequences of bulldog ants (Myrmecia: Formicidae). Chromosoma. 104 (1): 14-18
Michener CD. 2007. The bees of the world. The John Hopkins University Press, Baltimore, Maryland.
Mravinac B, Meštrovi? N, ?avrak VV, Plohl M. 2011. TCAGG, an alternative telomeric sequence in insects. Chromosoma. 120 (4): 367-376.
Pedro SRM. 2014. The stingless bee fauna in Brazil (Hymenoptera: Apidae). Sociobiology. 61 (4): 348-354.
Pereira JOP. 2006. Diversidade genética da abelha sem ferrão Melipona quinquefasciata baseada no sequenciamento das regiões ITS1 parcial e 18S do DNA ribossômico nuclear. Thesis, Universidade Federal do Ceará, Fortaleza, Ceará.
Pereira TTP, dos Reis ACCC, Cardoso DC, Cristiano MP. 2018. Molecular phylogenetic reconstruction and localization of the (TTAGG)n telomeric repeats in the chromosomes of Acromyrmex striatus (Roger, 1863) suggests a lower ancestral karyotype for leafcutter ants (Hymenoptera). Comp. Cytogenet. 12 (1): 13.
Piccoli MCA, Bardella VB, Cabral-de-Mello DC. 2018. Repetitive DNAs in Melipona scutellaris (Hymenoptera: Apidae: Meliponidae): chromosomal distribution and test of multiple heterochromatin amplification in the genus. Apidologie. 49 (4): 497-504.
Pinkel D, Straume T, Gray JW. 1986. Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83 (9): 2934-2938.
Ramírez SR, Nieh JC, Quental TB, Roubik DW, Imperatriz-Fonseca VL, Pierce NE. 2010. A molecular phylogeny of the stingless bee genus Melipona (Hymenoptera: Apidae). Mol. Phylogenet. Evol. 56 (2): 519-525.
Rasmussen C, Cameron AS. 2010. Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal. Biol. J. Linn. Soc. 99 (1): 206-232.
Rocha MP. 2002. Análises citogenéticas em abelhas do gênero Melipona Hymenoptera, Meliponini). Dissertation, Universidade Federal de Viçosa.
Rocha MP, Cruz MP, Fernandes A, Waldschmidt AM, Silva-Júnior JC, Pompolo SG. 2003. Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromossomes. Hereditas. 138 (2): 133-137.
Rocha MP, Pompolo SG. 1998. Karyotypes and heterochromatin variation (C-bands) in Melipona species (Hymenoptera, Apidae, Meliponinae). Genet. Mol. Biol. 21 (1): 41-45.
Rocha MP, Pompolo SG, Dergam JA, Fernandes A, Campos LAO. 2002. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). Hereditas. 136 (1): 19-27.
Rocha MP, Pompolo SG, Fernandes A, Campos LAO. 2007. Melipona: Six decade of cytogenetic. Biosc. J. 1: 111-117.
Sahara K, Marec F, Traut W. 1999. TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome Res. 7 (6): 449-460.
Schweizer D. 1980. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogen. Cell. Genet. 27 (6): 190-193.
Silva AA, Rocha MP, Pompolo SG, Campos LAO, Tavares MG. 2018. Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes. Comp. Cytogenet. 12 (4): 471.
Sumner AT. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res. 75 (1): 304-306.
Tavares MG, Lopes DM, Campos LAO. 2017. An overview of cytogenetics of the tribe Meliponine (Hymenoptera: Apidae). Genetica 145 (6): 1-18.
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Published
2019-12-05
How to Cite
Travenzoli, N. M., Barbosa, I. C. de O., Carvalho-Zilse, G. A., Salomão, T. M. F., & Lopes, D. M. (2019). Karyotypic description and repetitive DNA chromosome mapping of Melipona interrupta Latreille, 1811 (Hymenoptera: Meliponini). Caryologia, 72(2), 91–95. https://doi.org/10.13128/cayologia-239
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