Fluorescence In Situ Hybridisation Study of Micronuclei in C3A Cells Following Exposure to ELF-Magnetic Fields
Keywords:50 Hz magnetic fields, FISH staining, micronuclei, centromere staining, genotoxicity
Human C3A cells were exposed to extremely low frequency (50 Hz) magnetic fields (ELF-MF’s) up to 500 µT. They were subjected to the micronucleus assay using a Fluorescence In Situ Hybridization (FISH) technique with an in-house pan-centromere probe. We found no increased frequency in micronucleated cells and no change in the proportion of centromere positive over centromere negative micronuclei compared to the unexposed control cells. These results are in accordance with some, but in contradiction with other previously published investigations underlining that effects of environmental ELF-EMF’s on cellular DNA may be very subtle and that small changes or environmental influences may determine the outcome of a (geno)toxicity study. Interestingly, a low-level (5µT) exposure resulted in less than the background micronucleus frequency.
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