Karyotype diversity of stingless bees of the genus Frieseomelitta (Hymenoptera, Apidae, Meliponini)
DOI:
https://doi.org/10.13128/caryologia-610Keywords:
heterochromatin, karyotype evolution, centric fission, chromosome, variationAbstract
Frieseomelitta (Ihering, 1912) is a genus of stingless bees, distributed in the Nearctic and Neotropical regions. Specimens can be found in forests, cerrado, caatinga and mountainous regions. This genus has 16 species, of which 13 are recorded in Brazil. Cytogenetics has contributed to evolutionary studies of some Hymenoptera groups and although many Frieseomelitta species have been described, few species have been studied cytogenetically. The present study aims to contribute to the knowledge of the karyotype diversity of this genus, seeking to understand the possible evolutionary mechanisms that occurred in the diversification of the karyotype of this genus. Frieseomelitta portoi and Frieseomelitta trichocerata and Frieseomelitta doederleini showed diploid karyotypes with 2n = 30 chromosomes, similarly to all the species previously analyzed in the genus. Unprecedentedly, Frieseomelitta longipes showed 2n = 34. These results confirm that the frequent diploid number of 30 chromosomes is typical of this genus. The finding of 2n = 34 chromosomes in F. longipes comprises the first record of a diploid chromosome number different from 2n=30 in this group, which suggests that it can be the result of a recent chromosome change event. An interspecific comparative analysis was developed involving present and previous studies, as well as a discussion on the mechanisms involved in the karyotypic evolution in the genus.
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References
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Costa KF, Brito RM, Miyazawa CS. 2004. Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil. Genetics and Molecular Biology. Vol. 27 nº 2 São Paulo.
Crozier RH. 1975. Hymenoptera. In: B. John (ed), Animal Cytogenetics 3. Insecta 7. Berlin, Grebuder, Borntraeger. 95p.
Domingues AMT, Waldschmidt AM, Andrade SE, Souza VA, Alves RMO, Junior JCS, Costa MA. 2005. Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: Genetics and Molecular Biology, 28, 3, 390-393.
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Friese HFAKL. 1900. Neue Arten der Bienengattungen Melipona III., und Trigona Jur. Természetrajzi Fuzetek, 23(11): 381-394.
Friese HFAKL. 1902. Neue Meliponiden (Hym). Zeitschrift fur Systematische Hymenopterologie und Dipterologie, 2: 382-383.
Guerra MS, Souza MJ. 2002. Como Observar Cromossomos: Um Guia de Técnicas em Citogenética Vegetal, Animal e Humana. Ribeirão Preto, São Paulo, Brazil.
Hoshiba H. 1988. Karyological analysis of a stingless bee Melipona favosa (Apidae, Hymenoptera). Cytologia 53:153-156.
Hoshiba H, Imai HT. 1993. Chromosome evolution of bees and wasps (Hymenoptera, Apocrita) on the basis of C banding pattern analyses. Japonese Journal of Entomology 61:465-492.
Imai HT. 1988. Modes of spontaneous chromosomal mutation and karyotypes evolution in ants with reference to he minimum intraction hypothesis. Japonese Journal of Genetics 63: 159-185.
Imai HT. 1991. Mutability of constitutive heterochromatin (c-bands) during eukaryotic chromosomal evolution and their cytological meaning. Japonese Journal of Genetics 66: 653-661.
Imai HT, Taylor RW, Crosland MWJ, Crozier RH. 1988. Modes of spontaneous evolution in ants with reference to the minimium interaction hypothesis. Japonese Journal of Genetics 66: 159-185.
Imai HT, Maruyama T, Gojobori T, Inoue Y., Crozier RH. 1986. Theoretical bases for karyotype evolution. The minimum-interaction hypothesis. American Naturalist 128: 900-920.
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Imai HT, Brown WL, Kubota JRM, Young HS, Tho YP. 1984. Chromosome observations of tropical ants in western Malasya. Annual Report National Institute of Genetics 63: 66-69.
Imai HT, Taylor RW. 1989. Chromosomal polymorphism involving telomere fusion, centromeric inactivation and centromere shipt in the ant Myrmecia (pilosula) n=1. Chromosoma 98: 456-460.
Imai HT, Crozier RH, Taylor RW. 1977. Karyotype evolution in Australlian ants. Chromosoma 53: 341-393.
Imai HT, Kubota M. 1975. Chromosome polymorphism in the ant, Pheidole nodus. Chromosoma 51: 391-399.
Imai HT. 1991. Mutability of constitutive heterochromatin (c-bands) during eukaryotic chromosomal evolution and their cytological meaning. Japonese Journal of Genetics 66: 653-661.
Kerr WE. 1972a. Numbers of Chromosomes in Some Species of Bees. Journal of the Kansas Entomological Society, 45 (1): 111-122.
Kerr WE. 1972b. Karyotypic Evolution of Bees and Corresponding Taxonomic Implications. Evolution, 45 (1): 197-202.
Kerr WE, Silveira ZV. 1972. Karyotypc evolution of bees and corresponding taxonomic implications. Evolution 26: 197-202.
Lepeletier de Saint-Fargeau A. 1836. Histoire Naturalle des Insects Hyménopteres. nº 10, Bis. Librairie Encyclopédique de Roret, Paris. 547pp.
Mampumbu AR. 2002. Análise citogenética da heterocromatina e da NOR em Populações de abelhas sem ferrão Friesella schrottkyi (FRIESE, 1900) (Hymenoptera: Apidae: Meliponini). Dissertação de Mestrado, Unicamp, Campinas.
Martins CCC, Diniz D, Sobrinho-Scudeler PE, Foresti F, Campos LAO, Costa MA. 2012. Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting. Apidologie 44, 75–81
Menezes MBF. 1997. Caracterização citogenética e análise da heterocromatina constitutiva em Tetragonisca angustula (Hymenoptera, Meliponinae). Masters Thesis, Universidade Federal de Viçosa, Viçosa, Brazil.
Moure JS. 1946. Contribuição para o Conhecimento dos Meliponinae (Hym. Apoidea). Revista de Entomologia, 17(3): 437-443.
Moure JS. 1950. Notas Sobre Alguns Meliponinae Bolivianos (Hymenoptera, Apoidea). Dusenia, 2(1): 70-80.
Moure JS. 1963. Uma Nova Espécie de “Trigona (Frieseomelitta)” do Norte do Distrito Tupi (Hymenoptera, Apoidea). Revista Brasileira de Biologia, 23(1): 39-43.
Moure JS. 1989. Espécies Novas de Abelhas da Região Central do Estado de Minas Gerais, Brasil (Hymenoptera, Apoidea). Acta Biológica Paranaense 18(1, 2, 3, 4): 115-127.
Moure JS. 1988. Uma Nova Espécie de Frieseomelitta do Oeste da Amazônia (Hymenoptera, Apoidea). Acta Biológica Paranaense 17(1, 2, 3, 4): 141-145.
Oliveira FF. 2003. Revisão do gênero Frieseomelitta von Ihering, 1912 (Hymenoptera, Apidae, Meliponinae), com notas bionômicas de algumas Espécies. Tese de Doutorado, Universidade Federal do Paraná, Curitiba, 327p.
Pompolo SG, Campos LAO. 1995. Karyotypes of two species of sintgless bee, Leurotrigona muelleri and Leurotrigona pusilla (Hymenoptera, Meliponinae). Rev. Brasil. Genet. 18:181-184.
Rasmussen C, Cameron SA. 2010. Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal. Biological Journal of the Linnean Society, 99: 206–232.
Rocha MP, Pompolo SG. 1998. Karyotypes and heterochromatin variation (C-bands) in Melipona species (Hymenoptera, Apidae, Meliponinae). Genet. Mol. Biol. V. 21, p. 41-45.
Rocha MP, Pompolo SG, Dergam JÁ, Fernandes A, Campos LAO. 2002. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). Hereditas 136:19-37.
Rocha MP, Pompolo SG, Campos LAO. 2003. Citogenética da tribo Meliponini (Hymenoptera, Apidae). In: Melo, G. A. R. and Santos I. A. (eds) Apoidea Neotropica: Homenagem aos 90 anos de Jesus Santiago Moure. UNESC, Criciúma, pp 311-320.
Schweizer D. 1980. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogenet Cell Genet 27:190-193.
Silveira ZV, Kerr WE. 1971. Number of chromosomes in some Brazilian meliponids. (in press).
Silveira FA, Melo GAR, Almeida EAB. 2002. Abelhas Brasileiras – Sistemática e Identificação. Editora Composição e Arte, Minas Gerais, Brasil. 1ª edição. Pg 86.
Smith F. 1854. Catalogue of Hymenopterous Insects in the Collection of the British Museum: Part II, Apidae. Printed by Order of the Trustees, London. pp. 199-465.
Schwarz HF. 1940. Additional Species and Records of Stingless Bees (Meliponidae) from British Guiana. American Museum Novitates, 1078: 1-12p.
Carvalho AF, Costa MA. 2011. Cytogenetic characterization of two species of Frieseomelitta Ihering, 1912 (Hymenoptera, Apidae, Meliponini). Genetics and Molecular Biology (Impresso), v. 34, p. 237-239.
Camargo JMF, Pedro SRM. 2013. Meliponini Lepeletier, 1836. In Moure JS, Urban D and Melo GAR (Orgs). In Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region - online version. Available at http://www.moure.cria.org.br/catalogue. Accessed Agosto/14/2016.
Cockerell TDA. 1915. The Real Trigona dorsalis Smith rediscovered (Hym.) Entomological News, 26: 30-32.
Costa MA, Pompolo SG, Campos LAO. 1992. Supernumerary chromosomes in Partamona cupira (Hymenoptera, Apidae, Meliponinae). Rev. Bras. Genet. 15, 801–806.
Costa KF, Brito RM, Miyazawa CS. 2004. Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil. Genetics and Molecular Biology. Vol. 27 nº 2 São Paulo.
Crozier RH. 1975. Hymenoptera. In: B. John (ed), Animal Cytogenetics 3. Insecta 7. Berlin, Grebuder, Borntraeger. 95p.
Domingues AMT, Waldschmidt AM, Andrade SE, Souza VA, Alves RMO, Junior JCS, Costa MA. 2005. Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: Genetics and Molecular Biology, 28, 3, 390-393.
Fabricius JC. 1798. Suplementum Entomologiae Systematicae, Vol. 5 – Hafniae, Apud Proftet Stortch, 572pp.
Friese HFAKL. 1900. Neue Arten der Bienengattungen Melipona III., und Trigona Jur. Természetrajzi Fuzetek, 23(11): 381-394.
Friese HFAKL. 1902. Neue Meliponiden (Hym). Zeitschrift fur Systematische Hymenopterologie und Dipterologie, 2: 382-383.
Guerra MS, Souza MJ. 2002. Como Observar Cromossomos: Um Guia de Técnicas em Citogenética Vegetal, Animal e Humana. Ribeirão Preto, São Paulo, Brazil.
Hoshiba H. 1988. Karyological analysis of a stingless bee Melipona favosa (Apidae, Hymenoptera). Cytologia 53:153-156.
Hoshiba H, Imai HT. 1993. Chromosome evolution of bees and wasps (Hymenoptera, Apocrita) on the basis of C banding pattern analyses. Japonese Journal of Entomology 61:465-492.
Imai HT. 1988. Modes of spontaneous chromosomal mutation and karyotypes evolution in ants with reference to he minimum intraction hypothesis. Japonese Journal of Genetics 63: 159-185.
Imai HT. 1991. Mutability of constitutive heterochromatin (c-bands) during eukaryotic chromosomal evolution and their cytological meaning. Japonese Journal of Genetics 66: 653-661.
Imai HT, Taylor RW, Crosland MWJ, Crozier RH. 1988. Modes of spontaneous evolution in ants with reference to the minimium interaction hypothesis. Japonese Journal of Genetics 66: 159-185.
Imai HT, Maruyama T, Gojobori T, Inoue Y., Crozier RH. 1986. Theoretical bases for karyotype evolution. The minimum-interaction hypothesis. American Naturalist 128: 900-920.
Imai HT, Taylor RW, Crozier RH. 1994. Experimental bases for the minimium interaction theory. Chromosome evolution in ants of the Myrmecia pilosula species complex (Hymenoptera: Formicidae: Myrmecinae). Japonese Journal of Genetics 69: 137-182.
Imai HT, Brown WL, Kubota JRM, Young HS, Tho YP. 1984. Chromosome observations of tropical ants in western Malasya. Annual Report National Institute of Genetics 63: 66-69.
Imai HT, Taylor RW. 1989. Chromosomal polymorphism involving telomere fusion, centromeric inactivation and centromere shipt in the ant Myrmecia (pilosula) n=1. Chromosoma 98: 456-460.
Imai HT, Crozier RH, Taylor RW. 1977. Karyotype evolution in Australlian ants. Chromosoma 53: 341-393.
Imai HT, Kubota M. 1975. Chromosome polymorphism in the ant, Pheidole nodus. Chromosoma 51: 391-399.
Imai HT. 1991. Mutability of constitutive heterochromatin (c-bands) during eukaryotic chromosomal evolution and their cytological meaning. Japonese Journal of Genetics 66: 653-661.
Kerr WE. 1972a. Numbers of Chromosomes in Some Species of Bees. Journal of the Kansas Entomological Society, 45 (1): 111-122.
Kerr WE. 1972b. Karyotypic Evolution of Bees and Corresponding Taxonomic Implications. Evolution, 45 (1): 197-202.
Kerr WE, Silveira ZV. 1972. Karyotypc evolution of bees and corresponding taxonomic implications. Evolution 26: 197-202.
Lepeletier de Saint-Fargeau A. 1836. Histoire Naturalle des Insects Hyménopteres. nº 10, Bis. Librairie Encyclopédique de Roret, Paris. 547pp.
Mampumbu AR. 2002. Análise citogenética da heterocromatina e da NOR em Populações de abelhas sem ferrão Friesella schrottkyi (FRIESE, 1900) (Hymenoptera: Apidae: Meliponini). Dissertação de Mestrado, Unicamp, Campinas.
Martins CCC, Diniz D, Sobrinho-Scudeler PE, Foresti F, Campos LAO, Costa MA. 2012. Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting. Apidologie 44, 75–81
Menezes MBF. 1997. Caracterização citogenética e análise da heterocromatina constitutiva em Tetragonisca angustula (Hymenoptera, Meliponinae). Masters Thesis, Universidade Federal de Viçosa, Viçosa, Brazil.
Moure JS. 1946. Contribuição para o Conhecimento dos Meliponinae (Hym. Apoidea). Revista de Entomologia, 17(3): 437-443.
Moure JS. 1950. Notas Sobre Alguns Meliponinae Bolivianos (Hymenoptera, Apoidea). Dusenia, 2(1): 70-80.
Moure JS. 1963. Uma Nova Espécie de “Trigona (Frieseomelitta)” do Norte do Distrito Tupi (Hymenoptera, Apoidea). Revista Brasileira de Biologia, 23(1): 39-43.
Moure JS. 1989. Espécies Novas de Abelhas da Região Central do Estado de Minas Gerais, Brasil (Hymenoptera, Apoidea). Acta Biológica Paranaense 18(1, 2, 3, 4): 115-127.
Moure JS. 1988. Uma Nova Espécie de Frieseomelitta do Oeste da Amazônia (Hymenoptera, Apoidea). Acta Biológica Paranaense 17(1, 2, 3, 4): 141-145.
Oliveira FF. 2003. Revisão do gênero Frieseomelitta von Ihering, 1912 (Hymenoptera, Apidae, Meliponinae), com notas bionômicas de algumas Espécies. Tese de Doutorado, Universidade Federal do Paraná, Curitiba, 327p.
Pompolo SG, Campos LAO. 1995. Karyotypes of two species of sintgless bee, Leurotrigona muelleri and Leurotrigona pusilla (Hymenoptera, Meliponinae). Rev. Brasil. Genet. 18:181-184.
Rasmussen C, Cameron SA. 2010. Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal. Biological Journal of the Linnean Society, 99: 206–232.
Rocha MP, Pompolo SG. 1998. Karyotypes and heterochromatin variation (C-bands) in Melipona species (Hymenoptera, Apidae, Meliponinae). Genet. Mol. Biol. V. 21, p. 41-45.
Rocha MP, Pompolo SG, Dergam JÁ, Fernandes A, Campos LAO. 2002. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). Hereditas 136:19-37.
Rocha MP, Pompolo SG, Campos LAO. 2003. Citogenética da tribo Meliponini (Hymenoptera, Apidae). In: Melo, G. A. R. and Santos I. A. (eds) Apoidea Neotropica: Homenagem aos 90 anos de Jesus Santiago Moure. UNESC, Criciúma, pp 311-320.
Schweizer D. 1980. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogenet Cell Genet 27:190-193.
Silveira ZV, Kerr WE. 1971. Number of chromosomes in some Brazilian meliponids. (in press).
Silveira FA, Melo GAR, Almeida EAB. 2002. Abelhas Brasileiras – Sistemática e Identificação. Editora Composição e Arte, Minas Gerais, Brasil. 1ª edição. Pg 86.
Smith F. 1854. Catalogue of Hymenopterous Insects in the Collection of the British Museum: Part II, Apidae. Printed by Order of the Trustees, London. pp. 199-465.
Schwarz HF. 1940. Additional Species and Records of Stingless Bees (Meliponidae) from British Guiana. American Museum Novitates, 1078: 1-12p.
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Published
2020-04-13
How to Cite
do Nascimento, R. M., Carvalho, A. F., Santana, W. C., Barth, A., & Costa, M. A. (2020). Karyotype diversity of stingless bees of the genus Frieseomelitta (Hymenoptera, Apidae, Meliponini). Caryologia, 73(2), 121–126. https://doi.org/10.13128/caryologia-610
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