Meiotic behavior and its implications on the reproductive success of Arnebia euchroma (Royle ex Benth.) I.M.Johnst. (Boraginaceae), an important medicinal plant of Trans-Himalaya
DOI:
https://doi.org/10.36253/caryologia-2154Keywords:
chromosome number, meiotic abnormalities, pollen viability, seed setAbstract
The present study reports the chromosome number, meiotic behavior and its relation with pollen fertility and seed set of Arnebia euchroma (Royle ex Benth.) I.M.Johnst. The species shows a chromosome count of 2n = 2x = 14. The meiotic abnormalities such as chromatin stickiness, cytomixis, laggard formation, chromosomal bridges, were also observed in the Pollen Mother Cells (PMCs) of the target plant species. The linear model of regression showed a significant reduction of seed set with increasing meiotic abnormality and correlation analysis highlighted positive relationship between pollen viability and seed set. Meiotic abnormalities within the species hinder its reproductive process, causing a decline in reproductive efficiency. This study highlights the importance of addressing these intrinsic factors in future conservation programs to prevent a decline in the species population in nature.
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Arabi M, Norouzi M, Noori SAS, Mortazavian SMM. 2022. Chromosome Count, Male Meiotic Behavior and Pollen Analysis of Eight Populations of Trachyspermum ammi (L.) Sprague (Apiaceae) from Iran. Cytologia 87: 29-34.
Baquar, SR, Husain, SA. 1969. Cytoplasmic channels and chromatin migration in the meiocytes of Arnebia hispidissima (Sieb.) DC. Annals of Botany 33: 821-831.
Chaudhari AK, BR C. 2012. Meiotic chromosome behaviour and karyomorphology of Aloe vera (L.) Burm. f. Chromosome Botany. 7:23-9.
Cohen H, Philpott SM, Liere H, Lin BB, Jha S. 2021. The relationship between pollinator community and pollination services is mediated by floral abundance in urban landscapes. Urban Ecosystems 24: 275-290.
Dafni A. 1992. Pollination ecology. New York: Oxford University Press.
Dawson J, Wilson ZA, Aarts MGM, Braithwaite AF, Briarty, LG, Mulligan BJ. 1993. Microspore and pollen development in six male-sterile mutants of Arabidopsis thaliana. Canadian Journal of Botany 71: 629-638.
Falistocco E, Tosti N, Falcinelli M. 1995. Cytomixis in pollen mother cells of diploid Dactylis, one of the origins of 2n gametes. J. Hered 86: 448–453.
Gan X, Cao L, Zhang X, Li H. 2013. Floral biology, breeding system and pollination ecology of an endangered tree Tetracentron sinense Oliv.(Trochodendraceae). Botanical Studies, 54: 1-9.
Grewal A, Rani P. 2022. New record of chromosome count and B-chromosome in Tinantia erecta (Jacq.) Fenzl collected from different localities of Mussoorie, Uttarakhand (India). The Nucleus 65: 73-79.
Guan, JZ, Wang JJ, Cheng ZH, Liu Y Li ZY. 2012. Cytomixis and meiotic abnormalities during microsporogenesis are responsible for male sterility and chromosome variations in Houttuynia cordata. Genet Mol Res 11: 121-30.
Lal M, Samant SS, Kumar R, Sharma L, Paul S, Dutt S, Negi D, Devi K. 2020. Population ecology and niche modelling of endangered Arnebia euchroma in Himachal Pradesh, India-An approach for conservation. Med. Plants Int. J. Phytomed. Relat. Ind. 12: 90–104.
Lattoo SK, Khan S, Bamotra S, Dhar AK. 2006. Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thunb) Jacq.--an additional strategy and possible implications. Journal of Biosciences, 31: 5.
Lubbers AE, Christensen NL. 1986. Intraseasonal variation in seed production among flowers and plants of Thalictrum thalictroides (Ranunculaceae). Am. J. Bot. 73: 190–203.
Ma Y, Cui Z, Cheng CY, Li R, Wu H, Jin L, Wang Z. 2022. Flowering characteristics and mating system of Fritillaria cirrhosa (Liliaceae), an endangered plant in China. Brazilian Journal of Botany 45: 1307-1318.
Marks GE. 1954. An acetocarmine glycerol jelly for use in pollen fertility counts. Stain Technol. 29: 277.
Mursalimov S, Deineko E. 2017. Cytomixis in tobacco microsporogenesis: Are there any genome parts predisposed to migration? Protoplasma 254: 1379–1384.
Masoud S, Alijanpoo B, Khayyami M. 2010. Contribution to cytology of genus Salvia L.(Lamiaceae) in Iran. Caryologia 63:405-10.
Páez VDLA, Andrada AR, Kumar P Caro MS. 2021. Cytomixis in angiosperms from Northwestern Argentina. Botany Letters 168: 536-545.
Pessim C, Pagliarini MS, Silva N, Jank L. 2015. Chromosome stickiness impairs meiosis and influences reproductive success in Panicum maximum (Poaceae) hybrid plants. Genet. Mol. Res. 14: 4195-4202.
Rana PK, Kumar P, Singhal VK. 2013. Spindle irregularities, chromatin transfer, and chromatin stickiness during male meiosis in Anemone tetrasepala (Ranunculaceae). Turkish Journal of Botany. 37:167-76.
Rashid K, Rashid S, Ganie AH, Nawchoo, IA, Khuroo AA. 2021a. Meiotic studies, pollen fertility and seed set of Trillium govanianum, an endangered endemic plant species of the Himalaya. Cytologia 86: 245-249.
Rashid S, Rashid K, Ganie AH, Nawchoo IA, Khuroo AA. 2022b. Meiotic studies, pollen fertility and seed set of Actaea kashmiriana, an endemic medicinal plant species of Kashmir Himalaya. Cytologia 87: 239-244.
Schurr L, Affre L, Flacher F, Tatoni T, Le Mire Pecheux L, Geslin B. 2019. Pollination insights for the conservation of a rare threatened plant species, Astragalus tragacantha (Fabaceae). Biodiversity and Conservation 28: 1389-1409.
Sharma V, Gupta RC, Bala S. Singh B. 2013. New chromosome number reports in some medicinally important angiosperms of North India. Cytologia 78: 285-296.
Singh P, Tiwari S, Agrawal SB. 2022. Chromosomal and molecular indicators: a new insight in biomonitoring programs. InNew Paradigms in Environmental Biomonitoring Using Plants 317-340.
Singhal VK, Kumar P. 2008. Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle). Journal of Biosciences, 33: 371-380
Sofi II, Verma S, Ganie AH, Sharma N, Shah, MA. 2022a. Threat status of three important medicinal Himalayan plant species and conservation implications. Nature Conservation Research. 7: 1.
Sofi II, Zargar SA, Ganie AH, Shah MA. 2022b. Distribution dynamics of Arnebia euchroma (Royle) IM Johnst. and associated plant communities in Trans-Himalayan Ladakh region in relation to local livelihoods under climate change. Trees, Forests and People 7: 100213.
Tantray YR, Jan I, Wani MS, Singhal VK, Gupta RC. 2021. Chromosome numbers and meiotic behavior in some species of Asteraceae from high altitudinal regions of Kashmir Himalayas. Journal of Asia-Pacific Biodiversity, 14(4), 590-606.
Wani IA, Verma S, Ahmad P, El-Serehy HA, Hashim MJ. 2022. Reproductive Biology of Rheum webbianum Royle, a Vulnerable Medicinal Herb From Alpines of North-Western Himalaya. Frontiers in Plant Science, 13: 10.
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Copyright (c) 2023 Irfan Iqbal Sofi, Shivali Verma, Aijaz H Ganie, Namrata Sharma, Manzoor A Shah
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