Colchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae)

Authors

  • Harshita Dwivedi Plant Genetics Laboratory, Department of Botany, University of Allahabad, Prayagraj, 211002
  • Girjesh Kumar Plant Genetics Laboratory, Department of Botany, University of Allahabad, Prayagraj, 211002

DOI:

https://doi.org/10.36253/caryologia-1113

Keywords:

unreduced gametes, 2n pollen, polyploidy, colchicine, meiotic aberrations, Trachyspermum ammi (L.) Sprague

Abstract

Unreduced gametes are the key source for the natural polyploidization in plants, but rate of its formation is very low in nature. Meiotic mutants are second source for the formation of 2n pollen. In this cytological investigation, the meiotic aberrations and its impact on post-meiotic products were analysed in autotetraploid Trachyspermum ammi (L.) Sprague (4n=36). The seedlings of T. ammi (L.) Sprague were treated with 3 different concentrations of colchicine (0.2, 0.4 and 0.5%, w/v) for 3 different durations. Six polyploid plants were induced which was confirmed on the basis of cytological analysis. Colchicine, an anti-microtubular drug induced different meiotic and post-meiotic abnormalities such as chromosomal bridges, lagging chromosomes, scattering, precocious, fragments, dyads, triads, and polyads. The formation of several abnormal sporads clearly signifies the meiotic restitution. The tendency of univalents to scattered in the cytoplasm at metaphase was identified as a peculiar aberration asynapsis. Pollen variability and fusion of pollen walls was reported and pollen fertility was calculated. The morphological analysis of the pollen allowed us to confirm the occurrence of 2n pollen. 

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References

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Published

2021-12-21

How to Cite

Dwivedi, H., & Kumar, G. (2021). Colchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae). Caryologia, 74(3), 99-106. https://doi.org/10.36253/caryologia-1113

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