First cytogenetic characterization of the Amazon Catfish Leiarius marmoratus (Gill, 1870) and its hybrid with Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889)
DOI:
https://doi.org/10.36253/caryologia-1149Keywords:
Pimelodidae, Hybrid karyotype, Cachandiá, Pintado da Amazônia, Yaque, Ag-NORAbstract
This study reports the first cytogenetic characterization of the Amazonian catfish Leiarius marmoratus (“jandiá”) and its F1 (first generation) hybrid “cachandiá” with Pseudoplatystoma reticulatum (“cachara”). A diploid number of 56 chromosomes and a single argyrophilic nucleolus organizer region (Ag-NOR) in the short arm of two sub-telocentric chromosomes were observed for both L. marmoratus and P. reticulatum, but with differences in the karyotype formula and the size of the chromosome pair with NORs. The hybrid showed 2n = 56 chromosomes with an intermediate karyotype when compared to the parental species. A single Ag-NOR was maintained in the hybrid but located in two chromosomes with marked differences in size and presenting intraindividual variation in NOR activity (nucleolar dominance). For L. marmoratus and the hybrid, heterochromatic bands were predominately distributed in the terminal, centromeric, and sub-centromeric regions of some chromosomes and 5S rDNA sites located in two distinct sub-telocentric chromosomes, similar to the previously described for P. reticulatum. The data suggested that the hybrid karyotype might be insufficient for a precise discrimination of hybrids, however, Ag-NOR can be used as a chromosome marker to differentiate “cachandiá” from L. marmoratus and P. reticulatum. The current study also provides insights into the chromosomal features of L. marmoratus and contributes with novel cytogenetic information of this native Amazonian catfish included in the Pimelodidae family.
Downloads
References
Buitrago-Suarez UA, Burr BM. 2007. Taxonomy of the catfish genus Pseudoplatystoma Bleeker (Siluriformes: Pimelodidae) with recognition of eight species. Zootaxa 1512: 1-38.
Campos JL. 2010. O cultivo do pintado (Pseudoplatystoma corruscans, Spix; Agassiz, 1829), outras espécies do gênero Pseudoplatystoma e seus híbridos. In: Baldisserotto B, Gomes LC, editors. Espécies nativas para a piscicultura no Brasil. 1st ed. Santa Maria (RS): Universidade Federal de Santa Maria; p. 335–361.
Cioffi MB de, Moreira-Filho O, Ráb P, Sember A, Molina WF, Bertollo LAC. 2018. Conventional cytogenetic approaches - useful and indispensable tools in discovering fish biodiversity. Curr Genet Med Rep. 6: 176-186.
Feldberg E, Bertollo LAC. 1985. Nucleolar organizing regions in some species of Neotropical cichlid fish (Pisces, Perciformes). Caryologia. 38: 319-324.
Foresti F, Toledo LA. 1981. Polymorphic nature of nucleolus organizer regions in fishes. Cytogenet Genome Res. 31: 137-144.
Foresti F, Oliveira C, Almeida-Toledo LF. 1993. A method for chromosome preparations from large specimens of fishes using in vitro short treatment with colchicine. Experientia. 49: 810–813.
Girardi SC, Pavanelli CS, Margarido VP. 2018. Contributions to the systematic of Pimelodidae (Osteichthyes, Siluriformes): basic and molecular cytogenetics on seven species of Pimelodus from three Brazilian hydrographic systems. Neotrop Ichthyol. 16: e170148.
Grabowska AI, Boro? A, Kirtiklis L, Spóz A, Juchno D, Kotusz J. 2019. Chromosomal inheritance of parental rDNAs distribution pattern detected by FISH in diploid F1 hybrid progeny of Cobitis (Teleostei, Cobitidae) species has non?Mendelian character. J Fish Biol. 96: 261-273.
Hashimoto DT, Laudicina A, Bortolozzi J, Foresti F, Porto-Foresti F. 2009. Chromosomal features of nucleolar dominance in hybrids between the Neotropical fish Leporinus macrocephalus and Leporinus elongatus (Characiformes, Anostomidae). Genetica. 137: 135-140.
Hashimoto DT, Senhorini JA, Foresti F, Porto?Foresti F. 2012. Interspecific fish hybrids in Brazil: management of genetic resources for sustainable use. Rev Aquac. 4: 108-118.
Hashimoto DT, Prado FD, Foresti F, Porto-Foresti F. 2016. Molecular identification of intergenus crosses involving catfish hybrids: risks for aquaculture production. Neotrop Ichthyol. 14: e150139.
Howell WM, Black DA. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 36: 1014–1015.
Kasiroek W, Indananda C, Pinthong K, Supiwong W, Pengseng P, Tanomtong A. 2017. NOR Polymorphism and chromosome analysis of banggai cardinalfish, Pterapogon kauderni (Perciformes, Apogonidae). Cytologia. 82: 17-23.
Kubitza F, Ono EA, Campos JL. 2011. Alguns destaques da piscicultura em 2011. Revista Panorama da Aquicultura 21: 14-23.
Levan A, Fredga KE, Sandberg HA. 1964. Nomenclature from centromeric position on chromosomes. Hereditas. 52: 201–220.
Lundberg JG, Littmann MW. 2003. Pimelodidae (Long-whiskered catfishes). In: Reis RE, Kullander SO and Ferraris CJ Jr, editors. Checklist of the Freshwater Fishes of South and Central America. 1st ed. Porto Alegre (RS): EDIPUCRS, p. 432-446.
Mateo FJ, Delgado N, López H. 2008. Caracterización morfométrica del híbrido Yaque pintado (Pseudoplatystoma Fasciatum x Leiarius Marmoratus) y sus progenitores (Siluriformes: Pimelodidae). Revista de la Facultad de Ciencias Veterinarias. 49: 047-060.
Oliveira C, Almeida-Toledo LF, Foresti F, Toledo-Filho SA. 1988. Supernumerary chromosomes, Robertsonian rearrangements and multiple NORs in Corydoras aeneus (Pisces, Siluriformes, Callichthyidae). Caryologia. 41: 227-236.
Pendas AM, Moran P, Freije JP, Garcia-Vazquez E. 1994. Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet Cell Genet. 67: 31–36.
Pinkel D, Straume T, Gray JW. 1986. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Nat Acad Sci. 83: 2934–2938.
Porto-Foresti F, Andreatas AA, Oliveira C, Foresti F. 2000. The karyotype of Pseudoplatystoma fasciatum (Teleostei, Siluriformes) from the Rio Paraguay basin. Chromosome Sci. 4: 99–102.
Porto?Foresti F, Hashimoto DT, Prado FD, Senhorini JA, Foresti F. 2013. Genetic markers for the identification of hybrids among catfish species of the family Pimelodidae. J Appl Ichthyol. 29: 643-647.
Prado FD, Nunes TL, Senhorini JA, Bortolozzi J, Foresti F, Porto-Foresti F. 2012. Cytogenetic characterization of F1, F2 and backcross hybrids of the Neotropical catfish species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum (Pimelodidae, Siluriformes). Genet Mol Biol. 35: 57–64.
Sumner AT. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res. 75: 304–306.
Supiwong W, Tanomtong A, Supanuam P, Jantarat S, Khakhong S, Sanoamuang LO. 2012. A discovery of nucleolar organizer regions (NORs) polymorphism and karyological analysis of Smith's barb, Puntioplites proctozysron (Cypriniformes, Cyprinidae) in Thailand. Cytologia. 77: 35-42.
Swarça AC, Fenocchio AS, Dias AL. 2007. An update cytogenetic review for species of the family Pseudopimelodidae, Pimelodidae and Heptapteridae (Pisces, Siluriformes). Suggestion of a cytotaxonomical classification. Caryologia. 60: 338–348.
Swarça AC, Fenocchio AS, Cestari MM, Dias AL. 2008. Analyses of the structure of NORs in two species of South American Sorubiminae fishes (Siluriformes) by means of several cytogenetic techniques. Folia boil. 56: 31-35.
Swarça AC, Dias AL, Fenocchio AS. 2017. Cytogenetic studies in the redtail catfish, Phractocephalus hemioliopterus (Bloch & Schneider, 1801) (Siluriformes, Pimelodidae) a giant fish from Amazon basin. Comp Cytogenet. 11: 119-128.
Vicari MR, Almeida MCD, Bertollo LAC, Moreira-Filho O, Artoni RF. 2006. Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae). Genet Mol Biol. 29: 621-625.
Yabu MHS, Vidotto?Magnoni AP, Casimiro ACR, Garcia DAZ, Costa ADA, Prado FD, Porto-Foresti F, Orsi ML. 2018. First record of non?native hybrid catfish Pseudoplatystoma reticulatum × Leiarius marmoratus in the Upper Paraná River basin, Brazil. J Fish Biol. 92: 261-267.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Fernanda Dotti do Prado, Fabio Porto-Foresti, Andrea Abrigato de Freitas Mourão, Fausto Foresti, José Augusto Senhorini
This work is licensed under a Creative Commons Attribution 4.0 International License.
- Copyright on any open access article in a journal published byCaryologia is retained by the author(s).
- Authors grant Caryologia a license to publish the article and identify itself as the original publisher.
- Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.
- The Creative Commons Attribution License 4.0 formalizes these and other terms and conditions of publishing articles.
- In accordance with our Open Data policy, the Creative Commons CC0 1.0 Public Domain Dedication waiver applies to all published data in Caryologia open access articles.
