First Cytogenetic Characterization of the Amazon Catfish Leiarius marmoratus (Gill, 1870) and Its Hybrid with Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889)

Authors

  • Fernanda Dotti do Prado
  • Fabio Porto-Foresti Universidade Estadual Paulista, Departamento de Ciências Biológicas, Bauru, São Paulo, Brazil
  • Andrea Abrigato de Freitas Mourão
  • Fausto Foresti
  • José Augusto Senhorini

DOI:

https://doi.org/10.36253/caryologia-1149

Keywords:

Pimelodidae, Interspecific hybrid karyotype, Cachandiá, Pintado da Amazônia, Yaque, Ag-NOR

Abstract

This study reports the first cytogenetic characterization of the Amazonian catfish Leiarius marmoratus (“jandiá”) and its F1 (first generation or interspecific) hybrid “cachandiá” with Pseudoplatystoma reticulatum (“cachara”). A diploid number of 56 chromosomes and a single argyrophilic nucleolus organizer region (Ag-NOR) in the short arm of two sub-telocentric chromosomes were observed for both L. marmoratus and P. reticulatum, but with differences in the karyotype formula and the size of the chromosome pair with NORs. The hybrid showed 2n = 56 chromosomes with an intermediate karyotype when compared to the parental species. A single Ag-NOR was maintained in the hybrid but located in two chromosomes with marked differences in size and presenting intraindividual variation in NOR activity (nucleolar dominance). For L. marmoratus and the hybrid, heterochromatic bands were predominately distributed in the terminal, centromeric, and sub-centromeric regions of some chromosomes and 5S rDNA sites located in two distinct sub-telocentric chromosomes, which was the same pattern than previously described for P. reticulatum in other studies. These results corroborated the lack of hybrid chromosomal homology, with chromosomes originating from L. marmoratus and from P. reticulatum. Results showed that chromosome morphology might be insufficient to account for a clear discrimination of hybrids from the parental lineages, however, Ag-NOR can help for the identification of hybrid fishes. The current study provides insights into the chromosomal features of L. marmoratus, and its hybrid, and contributes novel information about the rich biodiversity of fishes in South America.

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Published

2021-06-05

How to Cite

Dotti do Prado, F. ., Porto-Foresti, F., Abrigato de Freitas Mourão, A., Foresti, F., & Augusto Senhorini, J. . (2021). First Cytogenetic Characterization of the Amazon Catfish Leiarius marmoratus (Gill, 1870) and Its Hybrid with Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889) . Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics. https://doi.org/10.36253/caryologia-1149

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