Ploidy level determination of Hedera (Araliaceae) with emphasis on discussable species (Hedera hibernica)
Genome size is a helpful tool to circumscribe taxa at diverse taxonomic degrees (mostly species) and resolve intricate low-level taxonomies. The correct genome size in Hedera (Araliaceae) has long been discussed and the ploidy level of some taxa is still unclear. 12 accessions of Hedera measured with flow cytometry (FCM). Flow cytometry is a relatively rapid, inexpensive and credible, tool. Fresh leaves of the Hedera samples and internal reference standard parsley (Petroselinum crispum) stained with Propidium Iodide (PI). Flow cytometry measurements showed that the accessions 2CV (3.09 - 6.40 pg), the lowest amount of nuclear DNA was 3.09 pg for Hedera crebrescens (So), while its highest amount of nuclear DNA for H. hibernica “Hamilton” was 6.40 pg, which is a statistically significant difference. According to this study, new taxon (H. crebrescens) would be diploid, previously this taxon was considered H. hibernica (tetraploied).
How to Cite
Copyright (c) 2021 fahimeh
This work is licensed under a Creative Commons Attribution 4.0 International License.
- Copyright on any open access article in a journal published byCaryologia is retained by the author(s).
- Authors grant Caryologia a license to publish the article and identify itself as the original publisher.
- Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.
- The Creative Commons Attribution License 4.0 formalizes these and other terms and conditions of publishing articles.
- In accordance with our Open Data policy, the Creative Commons CC0 1.0 Public Domain Dedication waiver applies to all published data in Caryologia open access articles.