Cytotoxic effects of %70 Thiophanate methyl fungicide
DOI:
https://doi.org/10.36253/caryologia-3626Keywords:
Thiophanate methyl, Triticum test, MTT assay, CytotoxicityAbstract
In this study, Triticum growth inhibition test was used to determine the effects of this fungicide on root and stem growth and % mitotic index. For this purpose, Kate A1 Russian wheat variety was used as test material. According to the Triticum root growth test, the concentration value that halves the root length is known as the 50 EC50 (effective concentration) value. According to the test, root length of the control group was 9.38 ± 0.66 cm and stem length was 9.56 ± 0.88 cm. According to Triticum test, the EC50 value of the fungicide was found to be approximately 5000 ppm. Some doses of this fungicide used (2500, 5000 and 10000 ppm) were observed to inhibit root and stem growth and all the results were statistically significant according to Dunnet-t test. % In the root mitotic index analysis studies, 5000 cells were counted for the doses and it was observed that the tested concentrations of 1250, 2500, 5000 and 10000 ppm decreased mitotic activity. It was observed that the concentration of 10000 ppm decreases the mitotic index (11.02 ± 2.35 cm) the most. The highest recommended dose of the tested fungicide in the fight against agricultural pests is 1000 ppm and the EC50 value is determined as 5000 ppm according to the test results indicating that the cytotoxic effects of this fungicide will be limited. In MTT assay, toxic effects were observed at all concentrations and time applications of 70% Thiophanate Methyl fungicide. Dose and time dependent decreases in cell viability were observed. These results show that the fungicide has a cytotoxic effect on MDBK cells at the doses used.
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