Antibacterial power of Pomegranate extracts against Beta-Lactamase producing Escherichia coli

Authors

  • Mashail A. Alghamdi Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Faisal Al-Sarraj Faisal Al-Sarraj
  • Wafa H. Alamshani Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Ibrahim Alotibi Health Information Technology Department, Applied College, King Abdulaziz University, Jeddah
  • Majid Al-Zahrani Biological Science Department, College of Science and Art, King Abdulaziz University, Rabigh
  • Raed Albiheyri Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Nada M. Nass Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Bayan H. Sajer Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Noor M. Bataweel Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Mohammed A. Al-Matary Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Thamer Bouback Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Dalya Attallah Clinical and Molecular Microbiology Laboratory, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah
  • Tahani M. Alqahtani Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah

DOI:

https://doi.org/10.36253/caryologia-2356

Keywords:

Punica granatum, E. coli, beta-lactamase enzyme, antibacterial activities, antimicrobial assay, DNA sequencing

Abstract

Herbs, as the pomegranate (Punica granatum L.)(P. granatum), has significant chemical constituents with distinct pharmacological properties. These chemicals confer neuroprotective, antioxidant, anticancer, anti-inflammatory, and antibacterial properties to the plant. Pomegranate has specific components that enable its pharmacological actions; one of the functions of pomegranate extracts is to deactivate what is called extended spectrum beta-lactamase (ESBL) that makes Escherichia coli (E. coli) resistant to standard antibiotics. Twenty E. coli strains identified as beta-lactamase producers, the strains isolated from urine samples collected from patients with symptomatic urinary tract infection (UTI) and molecularly characterised using 16S rDNA. The study evaluated the antibiotic sensitivity and antibacterial activities of acetone and ethanolic pomegranate leaf and peel extracts, assessing their antimicrobial susceptibility against nineteen antibiotics. The ehanolic peel (EP) and leave (EL) extracts showed inhibitory potential inhibition zones spanning (9.0-12.6-18.3 mm) against E. coli pathogen producing extended-spectrum beta-lactamase as compared with (10.2-15.3 mm) inhibition scale exhibited by acetone peel (AP) and leave (AL) extracts treatment. Pomegranate leaves and peel extracts contain bioactive compounds with antioxidant, antimicrobial, and other biological effects, and can be fractionated for the identification of new antibacterial bioactive compounds for the development of drugs against ESBL- E. coli, in addition to their synergy with antibiotics for combination therapy that may have effective management and treatment of multidrug-resistant infections such as urinary tract infection.

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Published

2024-07-08

How to Cite

Alghamdi, M. A., Al-Sarraj, F., Alamshani, W. H., Alotibi, I., Al-Zahrani, M., Albiheyri, R., Nass, N. M., Sajer, B. H., Bataweel, N. M., Al-Matary, M. A., Bouback, T., Attallah, D., & Alqahtani, T. M. (2024). Antibacterial power of Pomegranate extracts against Beta-Lactamase producing Escherichia coli. Caryologia, 77(1), 83–99. https://doi.org/10.36253/caryologia-2356

Issue

Vol. 77 No. 1 (2024)

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Copyright (c) 2024 Mashail A. Alghamdi, Faisal Al-Sarraj, Wafa H. Alamshani, Ibrahim Alotibi, Majid Al-Zahrani, Raed Albiheyri, Nada M. Nass, Bayan H. Sajer, Noor M. Bataweel, Mohammed A. Al-Matary, Thamer Bouback, Dalya Attallah, Tahani M. Alqahtani

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