Antibacterial power of Pomegranate extracts against Beta-Lactamase producing Escherichia coli


  • Mashail A. Alghamdi Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Faisal Al-Sarraj Faisal Al-Sarraj
  • Wafa H. Alamshani Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Ibrahim Alotibi Health Information Technology Department, Applied College, King Abdulaziz University, Jeddah
  • Majid Al-Zahrani Biological Science Department, College of Science and Art, King Abdulaziz University, Rabigh
  • Raed Albiheyri Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Nada M. Nass Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Bayan H. Sajer Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Noor M. Bataweel Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Mohammed A. Al-Matary Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Thamer Bouback Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah
  • Dalya Attallah Clinical and Molecular Microbiology Laboratory, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah
  • Tahani M. Alqahtani Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah



Punica granatum, E. coli, beta-lactamase enzyme, antibacterial activities, antimicrobial assay, DNA sequencing


Herbs, as the pomegranate (Punica granatum L.)(P. granatum), has significant chemical constituents with distinct pharmacological properties. These chemicals confer neuroprotective, antioxidant, anticancer, anti-inflammatory, and antibacterial properties to the plant. Pomegranate has specific components that enable its pharmacological actions; one of the functions of pomegranate extracts is to deactivate what is called extended spectrum beta-lactamase (ESBL) that makes Escherichia coli (E. coli) resistant to standard antibiotics. Twenty E. coli strains identified as beta-lactamase producers, the strains isolated from urine samples collected from patients with symptomatic urinary tract infection (UTI) and molecularly characterised using 16S rDNA. The study evaluated the antibiotic sensitivity and antibacterial activities of acetone and ethanolic pomegranate leaf and peel extracts, assessing their antimicrobial susceptibility against nineteen antibiotics. The ehanolic peel (EP) and leave (EL) extracts showed inhibitory potential inhibition zones spanning (9.0-12.6-18.3 mm) against E. coli pathogen producing extended-spectrum beta-lactamase as compared with (10.2-15.3 mm) inhibition scale exhibited by acetone peel (AP) and leave (AL) extracts treatment. Pomegranate leaves and peel extracts contain bioactive compounds with antioxidant, antimicrobial, and other biological effects, and can be fractionated for the identification of new antibacterial bioactive compounds for the development of drugs against ESBL- E. coli, in addition to their synergy with antibiotics for combination therapy that may have effective management and treatment of multidrug-resistant infections such as urinary tract infection.


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Alamshani HW, Al-Sarraj FM, Algamdi M. 2023. The inhibitory effect of Punica granatum on Escherichia coli and Klebsiella pneumonia extended spectrum β-lactamase strains. Novel Research in Microbiology Journal, 7(1):1836-56.

Alotibi I, Al-Sarraj FM, Albiheyri R, Alghamdi MA, Nass N, Bouback T, Sajer BH, Al-Zahrani M, Basingab F, Alharbi M. 2022. Study the apoptosis and necrosis inducing of fosfomycin into associated infected urothelial tissue by extended spectrum beta lactamase positive of E. coli. Microbial Pathogenesis. 173:105838.

Al-Sarraj FM. 2021a. The effect of antibiotics and photodynamic therapy on extended-spectrum beta-lactamase (ESBL) positive of Escherichia coli and Klebsiella pneumoniae in urothelial cells. Saudi J Biol Sci.28: (10):5561-7.

Al-Sarraj FM. 2021b. A Review on the impacts of Azadirachta indica on Multi-drug Resistant Extended Spectrum Beta Lactamase-positive of Escherichia coli and Klebsiella pneumonia. Adv Life Sci. 8(3):228-32.

Amanulla AM, Sundaram RJ. 2019. Green synthesis of TiO2 nanoparticles using orange peel extract for antibacterial, cytotoxicity and humidity sensor applications. Materials Today: Proceedings. 8:323-31.

Azcárate-Peril, M. A., & Raya, R. R. 2001. Methods for plasmid and genomic DNA isolation from lactobacilli. Food microbiology protocols. 135-139

Berthe T, Ratajczak M, Clermont O, Denamur E, Petit F. 2013. Evidence for coexistence of distinct Escherichia coli populations in various aquatic environments and their survival in estuary water. Appl environ microbiol, 79(15):4684-93.

Chauhan A, Jindal T, Chauhan A, Jindal T. 2020. Microbiological Culture Media: Types, Role and Composition. Microbiological Methods for Environment, Food and Pharmaceutical Analysis.23-66.

Church DL, Emshey D, Semeniuk H, Lloyd T, Pitout JD. 2007. Evaluation of BBL CHROMagar O157 versus Sorbitol-MacConkey medium for routine detection of Escherichia coli O157 in a centralised regional clinical microbiology laboratory. J Clin Microbiol. 45(9):3098-100.

Denamur E, Clermont O, Bonacorsi S, Gordon D. 2021. The population genetics of pathogenic Escherichia coli. Nature Reviews Microbiology. 19(1):37-54.

Di Sotto A, Locatelli M, Macone A, Toniolo C, Cesa S, Carradori S, Eufemi M, Mazzanti G, Di Giacomo S. 2019. Hypoglycemic, antiglycation, and cytoprotective properties of a phenol-rich extract from waste peel of Punica granatum L. var. Dente di Cavallo DC2. Molecules. 24(17)

Eryılmaz M, Bozkurt ME, Yildiz MM, Akin A. 2010. Antimicrobial resistance of urinary Escherichia coli isolates. Tropical Journal of Pharmaceutical Research, 9(2):205-209.

Facciola, Stephen. “Cornucopia—A Source Book of Edible Plants,” Kampong Publications, Vista, 1990. 677 pp. Paperback. (LC 90-92097, ISBN 0-9628087-0-9).

Fernández-Mazarrasa C, Mazarrasa O, Calvo J, del Arco A, Martínez-Martínez L. 2009. High concentrations of manganese in Mueller-Hinton agar increase MICs of tigecycline determined by Etest. Journal of Clinical Microbiology 47(3):827-9. Epub 2009 Jan 14. PMID: 19144806; PMCID: PMC2650928.

Flores-Mireles AL, Walker JN, Caparon M, Hultgren SJ. 2015. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol. 13(5):269-284.

George EF, Hall MA, Klerk GJ. 2008. Plant tissue culture procedure-background. Plant Propagation by Tissue Culture: Volume 1. The Background. 1-28.

Giri N, Lodhi A, Bisht DS, Bhoj S, Arya DK. 2021. Antibiotic Resistance of Escherichia Coli Isolated from Lake Nainital, Uttarakhand State, India. J Mountain Res. 16:127-36.

Hashemi SH, Hashemi N, Esna-Ashari F, Taher A, Dehghan A. 2017. Clinical features and antimicrobial resistance of bacterial agents of ventilator-associated tracheobronchitis in Hamedan, Iran. Oman Med J., 32(5):403-408.

Hashim I, Pharma S. 2013. Microbiological culture media in pharmaceutical industry. Foster city, USA: OMICS Group eBooks.

Heidari H, Hasanpour S, Ebrahim-Saraie HS, Motamedifar M. 2017. High incidence of virulence factors among clinical enterococcus faecalis isolates in Southwestern Iran. Infect Chemother, 49(1):51-56.

Ho PL, Chow KH, Yuen KY, Ng WS, Chau PY. 1998. Comparison of a novel, inhibitor-potentiated disc-diffusion test with other methods for the detection of extended-spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae. J Antimicrob Chemother 42(1):49-54.

Houri H, Kazemian H, Sedigh Ebrahim-Saraie H, Taji A, Tayebi Z, Heidari H. 2017. Linezolid activity against clinical Gram-positive cocci with advanced antimicrobial drug resistance in Iran. J Glob Antimicrob Resist. 10:200-203.

Huang Z, Peng J, Han J, Zhang G, Huang Y, Duan M, Liu D, Xiong Y, Xia S, Lai W. 2019. A novel method based on fluorescent magnetic nanobeads for rapid detection of Escherichia coli O157: H7. Food chemistry. 276:333-41.

Jam N, Hajimohammadi R, Gharbani P, Mehrizad A. 2022. Antibacterial activity of Punica granatum L. and Areca nut (PA) combined extracts against some food born pathogenic bacteria. Saudi J Biol Sci. 29(3):1730-6.

Johan A, Natalia A, Djauhari W, Effendi RF. 2017. Clinical and hemoglobin profile of malaria patients in Karitas Hospital, Southwest Sumba, and period of year. Indones J Trop Infect Dis. 8(1):1.

Joshi C, Patel P, Kothari V. 2019. Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens. F1000Research 8:70.

Joshi G V., Nair KK. 1960. Journal of Biological Sciences. J Ecol., 48(3):752.

Kam AH, Neergheen VS. 2022. Nutraceutical and phytopharmaceuticals in immune health. Innutrition and Functional Foods in Boosting Digestion, Metabolism and Immune Health, (pp. 445-475). Academic Press.

Kazemian H, Heidari H, Ghanavati R, Mohebi R, Ghafourian S, Shavalipour A, et al. 2016. Characterization of antimicrobial resistance pattern and molecular analysis among extended spectrum β-lactamase-producing Escherichia coli. Pharm Sci. 22(4):279-284.

Khalid M, Andreoli S. 2019. Extrarenal manifestations of the haemolytic uremic syndrome associated with Shiga toxin-producing Escherichia coli (STEC HUS). Paediatric Nephrology, 34:2495-507.

Khan N, Syed DN, Pal HC, Mukhtar H, Afaq F. 2012. Pomegranate fruit extract inhibits UVB-induced inflammation and proliferation by modulating NF-κB and MAPK signaling pathways in mouse skin. Photochem Photobiol. 88(5):1126-1134.

Kim YH, Choi EM. 2009. Stimulation of osteoblastic differentiation and inhibition of interleukin‐6 and nitric oxide in MC3T3‐E1 cells by pomegranate ethanol extract. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 23(5):737-9.

Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 35:1547-1549.

Kunle, Oluyemisi Folashade, Egharevba, Henry Omoregie and Ahmadu, Peter Ochogu. 2012. Standardization of herbal medicines - A review. Int. J. Biodivers. Conserv. 4(3), pp. 101-112

Lateef A., Oloke J. K., Gueguim-Kana E. B. 2004. Antimicrobial resistance of bacterial strains isolated from orange juice products. Afr J Biotechnol. 3(6):334-338.

Loizzo MR, Aiello F, Tenuta MC, Leporini M, Falco T, Tundis R. 2019. Pomegranate (Punica granatum L.). In Non vitamin and non mineral nutritional supplements Jan 1 (pp. 467-472). Academic Press.

Motamedifar M, Ebrahim-Saraie HS, Abadi AR, Moghadam MN. 2015. First outcome of MDR-TB among Co- Infected HIV/TB patients from South-West Iran. Tuberc Respir Dis (Seoul). 78(3):253-257.

Nurdin KE, Olla LRY, Feoh SF, et al. 2019. Effectively Test of 96% from Soe (Citrus sinensis L.) Sweet Orange Rind Ethanol Extract as Biolarvaside. J Info Kesehat. 17(2):176-183. 278.

Park SH, Ryu S, Kang DH. 2011. Improved selective and differential medium for isolation of Escherichia coli O157: H7. J Clinic Microb. 49(1):405-8.

Phillips GC, Garda M. 2019. Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology-Plant 55: 242-57.

Pourakbari B, Ferdosian F, Mahmoudi S, Teymuri M, Sabouni F, Heydari H, et al. 2012. Increase resistant rates and ESBLproduction between E. coli isolates causing urinary tract infection in young patients from Iran. Braz J Microbiol. 43(2):766-769.

Qi Z, Li G, Wang M, Chen C, Xu Z, An T. 2022. Photoelectrocatalytic inactivation mechanism of E. coli DH5α (TET) and synergistic degradation of corresponding antibiotics in water. Water Research. 215:118240.

Ricci D, Giamperi L, Bucchini A, Fraternale D. 2006. Antioxidant activity of Punica granatum fruits. Fitoterapia. 77(4):310-2.

Ellatif, SA, Nermine NA, Amira YM , Enayat MD, and Dalia KA 2021. Production, purification and identification of glycine,N-(m-anisoyl)-methyl ester from Pseudomonas aeruginosa with antimicrobial and anticancer activities. Kuwait j of science.48(3):1-12.

Ellatif, SA, Bouqellah, NA, Abu-Serie, MM. et al. 2022a. Assessment of probiotic efficacy and anticancer activities of Lactiplantibacillus plantarum ESSG1 (MZ683194.1) and Lactiplantibacillus pentosus ESSG2 (MZ683195.1) isolated from dairy products. Environ Sci Pollut Res.29 (26):39684-39701

Ellatif, SA., Abdel Razik, ES, Abu-Serie, M.M, Mahfouz A, Shater AF, Saleh FM, Hassan MM, Alsanie WF, Altalhi A, Daigham GE, et al. 2022b. Immunomodulatory Efficacy-Mediated Anti-HCV and Anti-HBV Potential of Kefir Grains; Unveiling the In Vitro Antibacterial, Antifungal, and Wound Healing Activities. Molecules 2022, 27, 2016.

Seeram NP, Adams LS, Henning SM, et al. 2005. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem. 16(6):360-367.

Shaikh S, Fatima J, Shakil S, Rizvi SM, Kamal MA. 2015. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi J Biol Sci. 22(1):90-101.

Shaygannia E, Bahmani M, Zamanzad B, Rafieian-Kopaei M. 2016. A Review Study on Punica granatum L. J Evid Based Complementary Altern Med. 21(3):221-227.

Sneath P.H.A. and Sokal R.R. 1973. Numerical Taxonomy. Freeman, San Francisco. Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.

Şuţan NA, Bărbuceanu M, Bărbuceanu D, and Deliu I 2023. Cytogenotoxic and antimicrobial effects of Nezara viridula (L.) (Hemip-tera: Heteroptera: Pentatomidae) alcoholic extracts. Caryologia 76(4): 39-49.

Stover ED, Mercure EW. 2007. The pomegranate: a new look at the fruit of paradise. Hort Science. 42(5):1088-92.

Tamura K., Nei M., and Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molec Biol Evol 28(10):2731-2739.

Warsi W, Sulistyani N. 2018. The Optimization of Secondary Metabolite Production Time and Screening Antibacterial Activity of Actinomycetes Isolate from Tin Plant Rizosfer (Ficus carica). J Teknol Lab. 7(1):15.

Wu S, Tian L. 2017. Diverse Phytochemicals and Bioactivities in the Ancient Fruit and Modern Functional Food Pomegranate (Punica granatum). Molecules. 22(10):1606.

Zam W, Khaddour A. 2017. Anti-virulence effects of aqueous pomegranate peel extract on E. coli urinary tract infection. Progr Nutr. 19:98-104.




How to Cite

Alghamdi, M. A., Al-Sarraj, F., Alamshani, W. H., Alotibi, I., Al-Zahrani, M., Albiheyri, R., Nass, N. M., Sajer, B. H., Bataweel, N. M., Al-Matary, M. A., Bouback, T., Attallah, D., & Alqahtani, T. M. (2024). Antibacterial power of Pomegranate extracts against Beta-Lactamase producing Escherichia coli. Caryologia, 77(1), 83–99.