An account of chromosomal damage in PMCs of stripe rust infected barley
DOI:
https://doi.org/10.36253/caryologia-2430Keywords:
anomalies, Barley, chromosomes, fungal mycotoxins, gamma rays, PucciniaAbstract
A study, on the effects of natural mild fungal infection on meiosis of Barley Pollen Mother Cells (PMCs), was done in order to analyze the chromosomal damage elicited by the pathogenic conditions. A pattern similar to common mutagens, of reduction in mitotic index and chiasma frequency, as well as, production of various aberrations that demonstrate chromosomal damage, was observed. The most common abnormalities were un-orientation and other spindle related aberrations, as well as stickiness and clumping of chromosomes. The disease induced a reduction in pollen viability as compared to the control plants. The results were compared with those of a high dose of a known mutagen ie gamma rays in order to draw commonalities between the two conditions.
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References
Agar, G. and L. Alpsoy (2005). Antagonistic effect of selenium against aflatoxin G1 toxicity. Botanical Bulletin of Academia Sinica 46:301-305.
Akhtar, N. (2014). Effect of physical and chemical mutagens on morphological behavior of tomato (Solanum lycopersicum) cv. “Rio grande” under heat stress conditions. Plant Breeding and Seed Sci. 70: 69-79.
Alam Q., Khan M.A. and Azaz Ahmad Z.R. (2022). Gamma Irradiation Induced Chromosome Aberrations in Meiotic Cells of Bread Wheat (Triticum aestivum L.). Int. J. Plant and Soil Sc. 34(5):81-89.
Albanese, R. (1982). The use of fertilized mouse eggs in detecting potential clastogens. Mutat. Res. 97:315-326.
Ananthaswamy H. N., Vakil U.K., Sreenivasan A. (1971). Biochemical and physiological changes in gamma-irradiated wheat during germination. Radiation Botany 11:1-12
Asare, A. T., Mensah, F., Acheampong, S., Asare-Bediako, E., and Armah, J. (2017). Effects of gamma irradiation on agromorphological characteristics of okra (Abelmoschus esculentus L. Moench.). Adv. Agric. 2017(3):1–7.
Bhat, T. A.; M. Sharma and M. Anis (2007b). Comparative Analysis of Meiotic Aberrations Induced by Diethylsulphate and Sodium Azide in Broad Bean (Vicia faba L.). Asian J. Plant Sciences, 6 (7):1051-1057.
Chen, T., Huang, L., Wang, M., Huang, Y., Zeng, R., Wang, X. (2020). Ethyl methyl sulfonate-induced mutagenesis and its effects on peanut agronomic, yield and quality traits. Agronomy 10(5):655-667.
Consolaro, M. E. L. and Pagliarini, M. S. (1996). Spontaneous Chromosome Stickiness in Microsporocytes of Centella asiatica (L.) Urban (Umbelliferae), Cytologia 61:57-61.
Cornforth M.N., Bedford J.S. and Bailey S.M. (2021). Radiation on Chromosomes: Sizing up the Damage. Cytogenet Genome Res 161(6-7):328–351.
Cremer, C. and Cremer, T. (1986). Induction of chromosome shattering by UV light and Caffeine: The influence of different distributions of photolesions. Mut. Res. 163:33-40.
Cremer, C., Cremer, T., Zorn, C. and Zimer, J. (1981). Induction of chromosome shattering by UV irradiation and caffeine: Comparison of whole-cell and partial cell radiation. Mut. Res. 84:331-348.
El-Diasty, Z. M., Zaied, K.A., Kosba, Z.A., Kash, K. S. and Abd El-Azez, M. H. (2009). Meiotic chromosomal anomalies resulting from fungal infection of maize compared with those resulting from gamma irradiation. J. Agric. Sci. Mansoura Univ., 34(9):9233-9248.
El-Naghy, M.A., Mazen, M.B. and Fadl-Allah, E.M. (1991). Studies on the fungus flora and aflatoxin production of cotton seeds in Egypt. Medical Journal of Islamic World Academy of Sciences. 4(2):141-145.
Evans, H. J. (1962). Chromosomal aberration induced ionizing radiations. Int. Rev. Cytol. 13:221-232
Gnankambary, K., Batieno, T. B. J., Sawadogo, N., Sawadogo, M., Yonli, D., and Ouédraogo, J. T. (2019). Assessment of radio-sensitivity for three cowpea genotypes to gamma irradiation. Int. J. Genet. Mol. Biol. 11(2):29-33.
Helmey, R. K. (2003). Cytological studies of some Fusarium toxins on growth, cell ultrastructure and electrophoretic profile of seed proteins in wheat and maize. Ph. D Thesis, Botany Department, Faculty of Science, Minia University, Minia, Egypt.
Jayabalan, N. and Rao, G. R. (1987). Gamma radiation induced cytological abnormalities in Lycopersicon esculentum Mill var. Pusa Ruby. Cytologia 52:1-4.
Jeong K. S. (2014). Effects of temperature-dependent optical properties on the fluence rate and temperature of biological tissue during low-level laser therapy. Lasers in Medical Science 29(2):637-644.
Kaur M., Chadha P., Kaur S., Kaur A., Kaur K., Yadav A. K., and Kaur R. (2018). Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura. Scientific Reports 8:4693.
Klasterska, I., Natarajn, A.T. and Ramel. C. (1976). An Interpretation of the origin of sub-chromatid aberrations and chromosome stickiness as a category of chromatid aberration. Hereditas 83:153-162.
Konzak C.F., Niknejad M., Wickham I. and Donaldson E. (1975). Mutagenic interaction of sodium azide on mutations induced in barley seeds treated with diethyl sulfate or N-methyl-N’-nitrosourea. Mut. Res. 30(1):55-61.
Kumar G. and Yadav. R.S. (2010). EMS induced genomic disorders in sesame (Sesamum indicum l.) Rom. J. Biol. – Plant Biol. 55(2):97-104.
Kumar, G and Singh, V. (2003). Comparative analysis of meiotic abnormalities induced by gamma rays and EMS in barley. J. Ind. Bot. Soc. 82:19-22.
Liu B., Tao X., and Dou Q (2022). Meiotic chromosomal abnormality detected in heterozygotes of Elymus nutans. Front. Plant Sci 13:3-9.
Luthra, J. K. And Chopra, V. L. (1990). “Genetics of stripe rust resistance in barley”, Ind. J. Genet. Plant Breeding 50(4):390-395.
Mc Gill, M., Pathak, S. and Hsu, T.C. (1974). Effect of Ethidium bromide on mitosis and chromosomes: A possible amatorial basis for chromosomal stickiness. Chromosoma 47:157-168.
Mehra, P. N. and Mann, A. C. (1974) Cytological effect of chemical mutagens on Pterotheca falconeria 1. Monofunctional alkylating agent. Nucleus 17:167-182
Mohandas, T and Grant, W. F. (1972). Cytogenetic effects of 2,4-D and amitrole in relation to nuclear volume and DNA content in some higher plants. - Can. J . Genet. Cytol. 14:773-783.
Motilal B., Jogeshwar, P., Mishra, R. R. and Rath, S. P. (2012). Analysis of EMS induced in vitro mutants of Asteracantha longifolia L. Nees using RAPD markers. Indian J. Biotechnology 11:39-47.
Nilan. R. A., Konzac, C. F., Heiner, R. E. and Froese-Gertzen, E. E. (1964) Chemical Mutagenesis in barley. Proc. In: Int Barley Genetics Symp.Washington pp. 35-54.
Pakorn, T., Taychasinpitak, T. Jompuk C. and Jompuk. P. (2009). Effects of Acute and Chronic Gamma irradiations on in vitro culture of Anubias congensis. J. Nat. Sci. 43:449-457.
Pereira, C. M., Taschetto, O. M., Defani-Scoarize, M. A. and Pagliarini, M. S. (1995). Spontaneous Chromosome Fragmentation in Maize Microsporocytes. Cytologia 60:297-301.
Permijit, K. and Grover, I. S. (1985). Cytological effects of some organophosphorus pesticides II. Meiotic effects. Cytologia 50:199-211.
Prasad, A. B. and Godward, M. B. E. (1969). Comparison of the developmental response of diploid and tetraploid Phalaris following irradiation. Rad. Bot 9:167-173.
Raghuvanshi, S.S. and Singh, A.K. (1974). Studies on effect of gamma rays on Trigonella foenum graecum L. Cytologia 39:473-482.
Rashid, K., Rashid, S., Ganie, A. H., Nawchoo, I. A., Khuroo, A. A. (2021). Meiotic Studies, Pollen Fertility and Seed Set of Trillium govanianum, an Endangered Endemic Plant Species of the Himalaya. Cytologia 86:245-249.
Roelfs, A.P. and Huerta-Espino, J. (1994). Seedling resistance in Hordeum vulgare to barley stripe rust from Texas. Plant Dis 78:1046-1049.
Selvakumar, R., Meeta, Madhu, Shekhawat, P.S., Verma, R.P.S. and Sharma, I. (2014). Management of stripe rust of barley using fungicides. Indian Phytopath. 67(2):138-142.
Singh, B., Mehta, S., Aggarwal, S. K. and Tiwari, M. (2019). Barley, Disease Resistance, and Molecular Breeding Approaches. In book: Disease Resistance in Crop Plants. Edition I, Springer, Cham; pp. 261-299.
Sinha S.K. & Mohapatra B.K., (1969). Compensatory chiasma formation in maize. Cytologia 34:523–527.
Sinha, S. S. N. & Roy. H. (1976). Distribution of chiasmata between and within nuclei in irradiated and non- irradiated population in Phaseolus sp. J. Cytol. Genet. 11:7-9.
Sohair, E. Antoinette, H. and Atif, H. (1989) Cytogenetic effects of herbicide Garlon 4 on root mitosis of Allium cepa. Cytologia 54:465-472.
Song, J. and Andrew, A. F. (2014). Microbial Pathogens Trigger Host DNA Double-Strand Breaks Whose Abundance Is Reduced by Plant Defense Responses. Plos Pathogens 10(6):1-16.
Sree Ramalu, K. (1973). Mutagenic effects of gamma rays, chemical mutagens and Combined treatments in Sorghum. P. Pfanzucitg. 68:287-293.
Srinivasachar H.R. (1979). Induction of segmental interchanges in pearl millet (Pennisetum typhoides). Theoretical and Applied Genetics 54(1):27-34.
Styer, C. H. and. Horace, G. C. (1984). Effects of Moniliformin on Mitosis in Maize (Zea mays L.). Plant and Cell Physiology 25(6):1077-1082.
Swanson, C. P. (1988). Variation: Nature and Consequences of Altered Chromosomal Structure. Cytogenetics, 2nd ed., Prentice Hall of India.
Turkoglu, A., Haliloglu, K., Tosun, M., Szulc, P., Demirel, F., Eren, B., Bujak, H., Karagoz, H., Selvet, M., Uzkan, G. and Niedbala, G. (2023). Sodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques. Agriculture 13(6):1242-1259.
Verma, R.P., Sharma, R.K. and Mishra, B. (2012). Barley Cultivars released in India. Names, Parentages, Origins and adaptations, Research Bulletin No. 29. Directorate of Wheat Research, Karnal.
Wani, A., and Anis, M. (2008). Gamma ray- and EMS-induced bold-seeded high-yielding mutants in chickpea (Cicer arietinum L.). T. J. Biol. 32:161-166.
Wilson A, Menon V, Khan Z, Alam A, Litovchick L, Yakovlev V. (2019). Nitric oxide-donor/PARP-inhibitor combination: A new approach for sensitization to ionizing radiation. Redox Biology 24:101-169.
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