Meiotic irregularities associated to cytomixis in Buddleja iresinoides (Griseb.) Hosseus. (Buddlejaceae) and Castilleja arvensis Schltdl. & Cham. (Orobanchaceae)
DOI:
https://doi.org/10.13128/caryologia-298Keywords:
Buddleja iresinoides, Castilleja arvensis, Chromatin, pollen mother cells, cytomixis, chromosome numbersAbstract
The current paper analyzes the male meiotic behavior in wild populations of Buddleja iresinoides and Castilleja arvensis from Piedmont areas of the Northwest Region of Argentina. Castilleja arvensis showed tetraploid number of chromosome of 2n = 24. Our results are not in agreement with the previously reported base number x = 19 for Buddleja and the chromosome number n = 28 found for B. iresinoides is atypical in the genus. Around 7 % pollen mother cells were aneuploid as they showed meiotic chromosome count of n = 20-21 bivalents. Possible origin for such atypical chromosome number has been discussed in this paper. During the cytological studies we also came across pollen mother cells showing meiotic abnormalities such as cytomixis, chromatin stickiness and anaphase bridges with lagging chromatin. Consequently microsporogenesis was also irregular showing dyads and triads. However, the percentage of these irregularities during meiosis and microsporogenesis was not higher, and pollen fertility was not affected to a great extent. Cytomixis and other meiotic abnormalities in these species are reported here for the first time.
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References
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Risueno MC, Gimenez-Martín G, Lopéz-Saez JF, Garcia MIR (1969) Connexions between meiocytes in plants. Cytologia 34: 262-272
Sáez FA (1960) The use of acetic or propionic hematoxylin for the study of chromosomes with the technique of crushing. Communication from the Biological Society. Montevideo. Mimeographed.
Sharma AK, Sharma A (1965) Chromosome techniques, theory and practice. Butteworth & Co., London. pp 474
Sheidai M, Fadaei F (2005) Cytogenetic studies in some species of Bromus L., section Genea Dum. J Genet 84: 189-194
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Sidorchuk YV, Deineko EV, Shumny VK (2007) Role of microtubular cytoskeleton and callose walls in the manifestation of cytomixis in pollen mother cells of tobacco Nicotiana tabacum L. Cell Tissue Biol 1: 577–581
Singhal VK, Kumar P (2008) Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle). J Biosci 33: 371-380
Singhal VK, Kumar P, Kaur D, Rana PK (2009) Chromatin transfer during male meiosis resulted into heterogeneous sized pollen grains in Anemone rivularis Buch.-Ham. ex DC. from Indian cold Deserts. Cytologia 74: 229–234
Tallent-Halsell NG, Watt MS (2009) The invasive Buddleja davidii (Butterfly Bush). Bot Rev 75: 292–325
Tank DC, Egger JM, Olmstead RC (2009) Phylogenetic classification of Subtribe Castillejinae (Orobanchaceae). Syst Bot 34: 182-197
Tank DC, Olmstead RC (2008) From annuals to perennials: phylogeny of subtribe Castillejinae (Orobanchaceae). Am J Bot 95: 608–625
Wang XY, Yu, CH, Li X, Wang CY, Zheng GC (2004) Ultrastructural aspects and possible origin of cytomictic channels providing intercellular connection in vegetative tissues of anthers. Russ J Plant Physiol 51:110-120
Wei-cheng Z, Wen-mei Y, Cheng-hou L (1988) The structural changes during the degeneration process of antipodal complex and its function to endosperm formation in wheat caryopsis. Acta Bot Sin 30: 457-462
Andrada AR, Páez VA (2014) Meiotic studies and pollen viability of three species of Tibouchina (Melastomataceae). Lilloa 51: 131-140 (in Spanish)
Basavaiah D, Murthy TCS (1987) Cytomixis in pollen mother cells of Urochloa panicoides P. Beauv. (Poaceae). Cytologia 52: 69-74
Bellucci M, Roscini C, Mariani A (2003) Cytomixis in Pollen Mother Cells of Medicago sativa L. J Hered 94: 512–516
Botta SM, Cabrera AL (1993) Scrophulariaceae. In: Cabrera AL (ed) Flora of the Province of Jujuy. Scientific Collection INTA 13: 155-226
Carrizo J, Isasmendi S (1994) Buddlejaceae. In: Flora of the Lerma Valley. Botanical Contributions of Salta. Flora Series: 1-10
Cooper DC (1952) The transfer of deoxyribose nucleic acid from the tapetum to the microsporocytes at the onset of meiosis. Am Nat 86:219-229
Chuang TI, Heckard LR (1982) Chromosome numbers of Orthocarpus and related monotypic genera (Scrophulariaceae: Subtribe Castillejinae). Brittonia 34: 89-101
Falistocco E, Tosti N, Falcinelli M (1995) Cytomixis in pollen mother cells of diploid Dactylis, one of the origins of 2n gametes. J Hered 86: 448–453
Gadella TWJ (1980) Cytology. In: Leeuwenberg AJM (ed), Engler and Prantl's 'Die natürlichen Pflanzenfamilien', Fam. Loganiaceae, vol. 28b (1), Duncker& Humblot, Berlin, pp 202-210 Gates RR (1911) Pollen formation in Oenothra gigas. Ann Bot 25. 909–940
Goldblatt P, Johnson DE (eds.) (1979+). Index to plant chromosome numbers (IPCN). http://www.tropicos.org/Project/IPCN (accessed 21 June 2018)
Guanq-Qin G, Gou-Chang Z (2004) Hypotheses for the functions of intercellular bridges in male germ cell development and its cellular mechanisms. J Theor Biol 229: 139–146
Guzicka M, Wozny A (2005) Cytomixis in shoot apex of Norway spruce [Picea abies (L.) Karst.]. Trees 18: 722–724
Heckard LR (1968) Chromosome numbers and polyploidy in Castilleja (Scrophulariaceae). Brittonia 20: 112-126.
Heckard LR, Chuang TI (1977) Chromosome numbers, polyploidy, and hybridization in Castilleja (scrophulariaceae) of the Great Basin and Rocky Mountains. Brittonia 29: 159-172
Heslop-Harrison J (1966) Cytoplasmic connections between angiosperm meiocytes. Ann Bot 30: 221-30
Körnicke M (1901) Uber Ortsveränderung von Zallkarnern. Sitzgsber. Niederrhein. Ges. Natur-u Heilk. zu. Bonn A., 14–25
Koul KK (1990) Cytomixis in Pollen Mother Cells of Alopecurus arundinaceus Poir. Cytologia 55: 169-173
Kumar P, Singhal VK, Kaur D, Kaur S (2010) Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis. Biol Plantarum 54: 181–184
Kumar P, Rana PK, Himshikha S, Singhal VK, Gupta RC (2013) Cytogeography and phenomenon of cytomixis in Silene vulgaris from cold regions of Northwest Himalayas (India). Plant Syst Evol 300: 831-842
Lozzia ME, Páez VA, Toranzo MI, Cristóbal ME (2009) Cytological Studies on Anemone decapetala Ard., Thalictrum decipiens Boivin and Thalictrum venturii Boivin (Ranunculaceae). Lilloa 46: 72-76
Malallah GA, Attia TA (2003) Cytomixis its possible evolutionary role in a Kuwaiti population of Diplotaxis harra (Brassicaceae). Bot J Linn Soc 143: 169-175
Norman EM (2000) Buddlejaceae. Flora Neotropica Monograph 81, New York Botanical Garden, New York, USA, pp 225
Núñez O (1968) An acetic-haematoxylin squash method for small chromosomes. Caryologia 21: 115-119
Oliveira-Pierre PM, Sousa SM (2011) Cytomixis in plants: causes, mechanisms and consequences. Rev Bras Biocienc. 9: 231-240
Ortíz AM, Seijo JG, Lavia GI (2006) Meiotic mechanisms involved in the reduction of the viability and in the variation of the morphology of the pollen grains of Arachis glabrata Benth. (Rhizomatosae section). Scientific and Technological Communications, Universidad Nacional del Nordeste, Summary, B-043
Oxelman B, Kornhall P, Norman EM (2004) Buddlejaceae. In: K. Kubitzki (ed) The families and genera of vascular plants. vol. 7, Berlin/Heidelberg/New York, pp 39-44
Páez V.A, Andrada AR, Muruaga NB (2013) Cytogenetic analysis and pollen viability in Rhipsalis lumbricoides (Cactaceae). Succulents 10: 16-20
Páez VA, Andrada AR, Lozzia ME (2013) Meiotic analysis of two species of Cuscuta subgenera Grammica (Convolvulaceae). Lilloa 50: 59-64
Risueno MC, Gimenez-Martín G, Lopéz-Saez JF, Garcia MIR (1969) Connexions between meiocytes in plants. Cytologia 34: 262-272
Sáez FA (1960) The use of acetic or propionic hematoxylin for the study of chromosomes with the technique of crushing. Communication from the Biological Society. Montevideo. Mimeographed.
Sharma AK, Sharma A (1965) Chromosome techniques, theory and practice. Butteworth & Co., London. pp 474
Sheidai M, Fadaei F (2005) Cytogenetic studies in some species of Bromus L., section Genea Dum. J Genet 84: 189-194
Sheidai M, Attaei S, Khosravi-Reineh M (2006) Cytology of some Iranian Stipa (Poaceae) species and populations. Acta Bot Croat 65: 1–11
Sidorchuk YV, Deineko EV, Shumny VK (2007) Role of microtubular cytoskeleton and callose walls in the manifestation of cytomixis in pollen mother cells of tobacco Nicotiana tabacum L. Cell Tissue Biol 1: 577–581
Singhal VK, Kumar P (2008) Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle). J Biosci 33: 371-380
Singhal VK, Kumar P, Kaur D, Rana PK (2009) Chromatin transfer during male meiosis resulted into heterogeneous sized pollen grains in Anemone rivularis Buch.-Ham. ex DC. from Indian cold Deserts. Cytologia 74: 229–234
Tallent-Halsell NG, Watt MS (2009) The invasive Buddleja davidii (Butterfly Bush). Bot Rev 75: 292–325
Tank DC, Egger JM, Olmstead RC (2009) Phylogenetic classification of Subtribe Castillejinae (Orobanchaceae). Syst Bot 34: 182-197
Tank DC, Olmstead RC (2008) From annuals to perennials: phylogeny of subtribe Castillejinae (Orobanchaceae). Am J Bot 95: 608–625
Wang XY, Yu, CH, Li X, Wang CY, Zheng GC (2004) Ultrastructural aspects and possible origin of cytomictic channels providing intercellular connection in vegetative tissues of anthers. Russ J Plant Physiol 51:110-120
Wei-cheng Z, Wen-mei Y, Cheng-hou L (1988) The structural changes during the degeneration process of antipodal complex and its function to endosperm formation in wheat caryopsis. Acta Bot Sin 30: 457-462
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Published
2019-12-23
How to Cite
Andrada, A. R., Páez, V. de los Ángeles, Caro, M., & Kumar, P. (2019). Meiotic irregularities associated to cytomixis in Buddleja iresinoides (Griseb.) Hosseus. (Buddlejaceae) and Castilleja arvensis Schltdl. & Cham. (Orobanchaceae). Caryologia, 72(4), 41–50. https://doi.org/10.13128/caryologia-298
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