Antiproliferative analysis of aqueous extracts of cabreúva (Myrocarpus frondosus) on the Allium cepa cell cycle
DOI:
https://doi.org/10.13128/caryologia-776Keywords:
Myrocarpus frondosus, medicinal plant, mitotic indexAbstract
Myrocarpus frondosus is widely used in Brazilian folk medicine for bronchitis, gastritis, ulcers and wound asepsis. However, there is a scarcity of scientific data proving the safe use of tea of this species or if there is any toxic effect on the human organism. This study aimed to evaluate the antiproliferative effect of aqueous extracts of cabreúva on the Allium cepa cell cycle. The aqueous extracts were prepared from leaves, bark and roots (dried material) of the species in two concentrations: 2.5 and 5.0 grams in 250 mL of distilled water. The aqueous extract of the leaves was obtained by infusion and the aqueous extract of the bark and roots by decoction. Distilled water was used as negative control and glyphosate 2% as positive control. Eight groups of four onion bulbs were evaluated and each group corresponded to one treatment and, in each group, 4000 cells were analyzed and the mitotic index was calculated. The results demonstrated reduction of mitotic indices in all treatments when compared to the negative control in water. The aqueous extracts of cabreúva in the studied concentrations have antiproliferative action on the Allium cepa cell cycle, ensuring the safe use of tea of this medicinal species.
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References
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Melo JG, Araújo TAS, Almeida Castro VTN, Cabral DLV, Rodrigues MD, Nascimento SC, Amorim ELC, Albuquerque UP. 2010. Antiproliferative activity, antioxidant capacity and tannin content in plants of semi-arid northeastern Brazil. Molecules. 15(12): 8534-8542. https://doi.org/10.3390/molecules15128534
Pereira Júnior LR, Andrade AP, Araújo KD, Barbosa AS, Barbosa FM. 2014. Espécies da Caatinga como alternativa para o desenvolvimento de novos fitofármacos. Floram. 21: 509-520. https://doi.org/10.1590/2179-8087.024212
Rodrigues LG, Tedesco SB, Trapp KC, Rosa VS. 2017. Efeito proliferativo de extratos aquosos de Lavandula angustifolia sob o ciclo celular de Allium cepa. In: 32° Jornada Acadêmica Integrada, Santa Maria, RS, Brasil. https://portal.ufsm.br/jai/trabalho/trabalho.html?action=anais
Romano B, Pagano E, Montanaro V, Fortunato AL, Milic N., Borrelli F. 2013. Novel insights into the pharmacology of flavonoids. Phytother. Res. 27(11): 1588-1596. https://doi.org/10.1002/ptr.5023
Santi II, Gatto DA, Machado MRG, Santos PSB, Freitag RA. 2017. Chemical composition, antioxidant and antimicrobial activity of the oil and plant extract Myrocarpus frondosus Allemão. Am. J. Plant Sci. 8(7): 1560-1571. https://doi.org/10.4236/ajps.2017.87108
Sccoti MSV, Araujo MM, Wendler CF, Longhi SJ. 2011. Mecanismos de regeneração natural em remanescente de Floresta Estacional Decidual. Ci. Fl. 21: 459-472. https://doi.org/10.5902/198050983803
Sehgal R, Roy S, Kumar VL. 2006. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allium cepa root model. Biocell.30(1): 9-13. PMID: 16845823
Tedesco SB, Laughinghouse IV HD. 2012. Bioindicator of genotoxicity: the Allium cepa test. In: Environmental Contamination, InTech Publisher. 137-156. https://doi.org/10.5772/31371
Trentin DS, Silva DB, Amaral MW, Zimmer KR, Silva MV, Lopes NP, Giordani RB, Macedo AJ. 2013. Tannins possessing bacteriostatic effect impair Pseudomonas aeruginosa adhesion and biofilm formation. Plos One. 8(6): 1-13. https://doi.org/10.1371/journal.pone.0066257
Tsai PH, Cheng CH, Lin CY, Huang YT, Lee LT, Kandaswami CC, Lin YC, Lee KP, Hung CC, Hwang JJ, Ke FC, Chang GD, Lee MT. 2016. Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells. Anticancer Res. 36(12): 6367-6380. https://doi.org/10.21873/anticanres.11234
Ubessi C, Tedesco SB, Silva CB, Baldoni M, Krysczun DK, Heinzmann BM, Rosa IA, Mori NC. 2019. Antiproliferative potential and phenolic compounds of infusions and essential oil of chamomile cultivated with homeopathy. J. Ethnopharmacol. 239: 1-7. https://doi.org/10.1016/j.jep.2019.111907
Vieira A, Guimarães MA, David GQ, Karsburg IV, Campos ANR. 2009. Efeito genotóxico da infusão de capítulos florais de camomila. R. Trop.: Ci. Agr. Biol. 3: 8-13. https://www.researchgate.net/publication/246044615_Evaluation_of_genotoxic_effects_of_chamomile_floral_chapters_infusion_Efeito_genotoxico_da_infusao_de_capitulos_florais_de_camomila
Bastos IVGA, Silva GKC, Rodrigues GCR, Melo CM, Xavier HS, Souza IA. 2011. Estudo fitoquímico preliminar e avaliação da toxicidade aguda do extrato etanólico bruto de Caesalpinia echinata Lam. Rev. Bras. Farm. 92: 219-222. http://www.rbfarma.org.br/files/rbf-2011-92-3-23.pdf
Bottamedi M, Nascimento MVPS, Fratoni E, Moon YJK, Dalmarco EM, Mendes BG. Evaluation of antioxidant and anti-inflammatory action (in vivo and in vitro) from the trunk barks of Myrocarpus frondosus Allemão (Cabreúva). In: 25° Simpósio de Plantas Medicinais do Brasil, São Paulo/Brasil, 2018. http://www.eventus.com.br/plantasmedicinais2018/anais_xxv_simposio_plantas_medicinais_2018.pdf
Cabrera DC, Gomes GLS, Schmidt N, Flach A, Costa LAMA, Rosa GR, Moura NF. 2012. Composição química das folhas da espécie Myrocarpus frondosus do sul do Brasil. In: 35ª Reunião Anual da Sociedade Brasileira de Química, São Paulo/Brasil. http://sec.sbq.org.br/cdrom/35ra/resumos/T0374-1.pdf
Caritá R, Marin-Morales MA. 2008. Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes. Chemosphere. 72: 722-725. https://doi.org/10.1016/j.chemosphere.2008.03.056
Coelho APD. 2013. Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae). [Dissertação] - Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Fachinetto JM, Tedesco SB. 2009. Atividade antiproliferativa e mutagênica dos extratos aquosos de Baccharis trimera (Less.) e Baccharis articulata (Lam.) Pers. (Asteraceae) sobre o sistema teste de Allium cepa. Rev. Bras. Plantas Med. 11: 360-367. https://doi.org/10.1590/s1516-05722009000400002
Freitas JMB, Tedesco SB, Rodrigues LG, Pasqualli M. 2016. Avaliação do potencial antiproliferativo do extrato de Lavandula angustifolia pelo teste de Allium cepa. In: 31° Jornada Acadêmica Integrada, Santa Maria/RS/Brasil, 2016. https://portal.ufsm.br/jai/trabalho/trabalho.html?action=anais
Frescura VD, Laughinghouse IV HD, Tedesco SB. 2012. Antiproliferative effect of the tree and medicinal species Luehea divaricata on the Allium cepa cell cycle. Caryologia. 65(1): 27-33. https://doi.org/10.1080/00087114.2012.678083
Gibellini L, Pinti M, Nasi M, Montagna JP, Biasi S, Roat E, Bertoncelli L, Cossarizza A. 2011. Quercetin and cancer chemoprevention. Evid.-Based Complementary Altern. Med. 1-15. https://doi.org/10.1093/ecam/neq053
Guerra ACVA, Soares LAL, Ferreira MRA, Araújo AA, Rocha HAO, Medeiros JS, Cavalcante RDS, Araújo Júnior RF. 2017. Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line. Biomed. Pharmacother. 92: 696-706. https://doi.org/10.1016/j.biopha.2017.05.123
Guerra M, Souza MJ. 2002. Como observar cromossomos: um guia de técnicas em citogenética vegetal, animal e humana. Fundação de Pesquisas Científicas de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
Kuhn AW. 2015. Viabilidade polínica, genotoxicidade, efeito antiproliferativo e compostos fenólicos de Peltodon longipes Kunth Ex Benth. (Lamiaceae). [Dissertação] - Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Leme DM, Marin-Morales MA. 2009. Allium cepa test in environmental monitoring: A review on its application. Mutat. Res. 682(1): 71-81. https://doi.org/10.1016/j.mrrev.2009.06.002
Lima RDF, Alves É P, Rosalen PL, Ruiz ALTG, Duarte MCT, Góes VFF, Medeiros ACD, Pereira JV, Godoy GP, Costa EMMB. 2014. Antimicrobial and antiproliferative potential of Anadenanthera colubrina (Vell.) Brenan. Evid.-Based Complementary Altern. Med. 1-7. https://doi.org/10.1155/2014/802696
Lorenzi H, Matos FJA. 2008. Plantas medicinais no Brasil: Nativas e Exóticas. 2ª ed. Nova Odessa - SP, Instituto Plantarum, Brasil.
Melo JG, Araújo TAS, Almeida Castro VTN, Cabral DLV, Rodrigues MD, Nascimento SC, Amorim ELC, Albuquerque UP. 2010. Antiproliferative activity, antioxidant capacity and tannin content in plants of semi-arid northeastern Brazil. Molecules. 15(12): 8534-8542. https://doi.org/10.3390/molecules15128534
Pereira Júnior LR, Andrade AP, Araújo KD, Barbosa AS, Barbosa FM. 2014. Espécies da Caatinga como alternativa para o desenvolvimento de novos fitofármacos. Floram. 21: 509-520. https://doi.org/10.1590/2179-8087.024212
Rodrigues LG, Tedesco SB, Trapp KC, Rosa VS. 2017. Efeito proliferativo de extratos aquosos de Lavandula angustifolia sob o ciclo celular de Allium cepa. In: 32° Jornada Acadêmica Integrada, Santa Maria, RS, Brasil. https://portal.ufsm.br/jai/trabalho/trabalho.html?action=anais
Romano B, Pagano E, Montanaro V, Fortunato AL, Milic N., Borrelli F. 2013. Novel insights into the pharmacology of flavonoids. Phytother. Res. 27(11): 1588-1596. https://doi.org/10.1002/ptr.5023
Santi II, Gatto DA, Machado MRG, Santos PSB, Freitag RA. 2017. Chemical composition, antioxidant and antimicrobial activity of the oil and plant extract Myrocarpus frondosus Allemão. Am. J. Plant Sci. 8(7): 1560-1571. https://doi.org/10.4236/ajps.2017.87108
Sccoti MSV, Araujo MM, Wendler CF, Longhi SJ. 2011. Mecanismos de regeneração natural em remanescente de Floresta Estacional Decidual. Ci. Fl. 21: 459-472. https://doi.org/10.5902/198050983803
Sehgal R, Roy S, Kumar VL. 2006. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allium cepa root model. Biocell.30(1): 9-13. PMID: 16845823
Tedesco SB, Laughinghouse IV HD. 2012. Bioindicator of genotoxicity: the Allium cepa test. In: Environmental Contamination, InTech Publisher. 137-156. https://doi.org/10.5772/31371
Trentin DS, Silva DB, Amaral MW, Zimmer KR, Silva MV, Lopes NP, Giordani RB, Macedo AJ. 2013. Tannins possessing bacteriostatic effect impair Pseudomonas aeruginosa adhesion and biofilm formation. Plos One. 8(6): 1-13. https://doi.org/10.1371/journal.pone.0066257
Tsai PH, Cheng CH, Lin CY, Huang YT, Lee LT, Kandaswami CC, Lin YC, Lee KP, Hung CC, Hwang JJ, Ke FC, Chang GD, Lee MT. 2016. Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells. Anticancer Res. 36(12): 6367-6380. https://doi.org/10.21873/anticanres.11234
Ubessi C, Tedesco SB, Silva CB, Baldoni M, Krysczun DK, Heinzmann BM, Rosa IA, Mori NC. 2019. Antiproliferative potential and phenolic compounds of infusions and essential oil of chamomile cultivated with homeopathy. J. Ethnopharmacol. 239: 1-7. https://doi.org/10.1016/j.jep.2019.111907
Vieira A, Guimarães MA, David GQ, Karsburg IV, Campos ANR. 2009. Efeito genotóxico da infusão de capítulos florais de camomila. R. Trop.: Ci. Agr. Biol. 3: 8-13. https://www.researchgate.net/publication/246044615_Evaluation_of_genotoxic_effects_of_chamomile_floral_chapters_infusion_Efeito_genotoxico_da_infusao_de_capitulos_florais_de_camomila
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2021-05-19
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Gonçalves Rodrigues, L., de Senna Pereira, J., Mena Barreto de Freitas, J., Ubessi, C., & Bosio Tedesco, S. (2021). Antiproliferative analysis of aqueous extracts of cabreúva (Myrocarpus frondosus) on the Allium cepa cell cycle. Caryologia, 73(4), 39–44. https://doi.org/10.13128/caryologia-776
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Copyright (c) 2020 Cassiane Ubessi, Luísa Gonçalves Rodrigues, Julia de Senna Pereira, Jéssica Mena Barreto de Freitas, Solange Bosio Tedesco
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