Karyological studies of four species of Lady’s Slipper Orchids (Paphiopedilum) collected in the Bogor Botanical Garden, Indonesia

Authors

  • Titien Ngatinem Praptosuwiryo Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911
  • R. Vitri Garvita Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911
  • Elizabeth Handini Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911
  • Izu Andry Fijridiyanto Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911 https://orcid.org/0000-0003-2999-606X
  • Joko Ridho Witono Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911 https://orcid.org/0000-0003-0946-0559

DOI:

https://doi.org/10.36253/caryologia-2232

Keywords:

Bogor Botanical Garden, chromosome, diploid, karyotype, Paphiopedilum

Abstract

Paphiopedilum is one of the most widely grown and hybridized of all orchid genera due to its distinctive flower morphology. This genus consists of 139 accepted species and is native to southern China to tropical Asia. Karyological studies on the genus Paphiopedilum have been reported by many cytologists in different countries. However, many Indonesian members of Paphiopedilum have remained comparatively limited in investigated cytologically. This study aimed to analyze karyological characters of four species of Paphiopedilum collected in the Bogor Botanical Garden, Indonesia, namely P. armeniacum, P. hirsutissimum, P. primulinum, and P. superbiens. Karyological studies were conducted by root tips squash method. The results showed that four species of Paphiopedilum have a basic chromosome number of x = 15. They are diploid with 2n=30, instead of the common diploid chromosome number of Paphiopedilum (2n = 26). Paphiopedilum hirsutissimum and P. primulinum possess 16 m + 14 sm chromosome formulae. Whereas Paphiopedilum armeniacum and P. superbiens revealed 16 m + 14 sm and 18 m + 12 sm chromosome formulae, respectively.A Robertsonian change in chromosome number generated by the fission of chromosomes would best explain the origin of the new diploid chromosome number and karyotypes of these species.

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Author Biographies

R. Vitri Garvita, Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911

  1. Vitri Garvita, is a researcher at the Research Center for Plant Conservation, Botanic Gardens, and Forestry, National Agency for Research and Innovation (BRIN), Indonesia. She received her master’s in Biology from Bogor Agricultural University (IPB University). Her particular research interests are in tissue culture and biological reproduction, especially in orchids. She has published many scientific papers on the tissue culture, ecology, and stomata of orchids.

Elizabeth Handini, Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911

Elizabeth Handini, is a researcher at the Research Center for Plant Conservation, Botanic Gardens, and Forestry, National Research and Innovation Agency, Indonesia. She received her master’s in Biology from Bogor Agricultural University (IPB University). Her particular research interests are in tissue culture and biological reproduction, especially in orchids. She has published many scientific papers dealing with the tissue culture of orchids

Izu Andry Fijridiyanto, Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911

Izu Andry Fijridiyanto, Dr., is a senior researcher at the Research Center for Biosystematics and Evolution, National Agency for Research and Innovation (BRIN). He received his doctorate in Plant Molecular Systematics from Kyoto University, Japan. His particular research interest is in plant molecular systematics and plant conservation, especially in the Lauraceae family. He has published several scientific papers in national and international journals. He is an editor of Jurnal Biologi Indonesia and a reviewer of some international and national journals. https://orcid.org/0000-0003-2999-606X

Joko Ridho Witono, Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km. 46, Cibinong, West Java 16911

Joko Ridho Witono, Dr. is a senior researcher at the Research Center for Biosystematics and Evolution, National Agency for Research and Innovation (BRIN). He received his doctorate in Plant Systematics from Hiroshima University, Japan. His particular research interest is in plant systematics and plant conservation, especially on palms (Palmae). He has published many scientific papers in international journals. He is a chief editor of The Botanic Gardens Bulletin, an editor and a reviewer of Biodiversitas Journal (Scopus Indexed), and reviewer some of international and national journals.  https://orcid.org/0000-0003-0946-0559

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Published

2024-07-08

How to Cite

Praptosuwiryo, T. N., Garvita, R. V., Handini, E., Fijridiyanto, I. A., & Witono, J. R. (2024). Karyological studies of four species of Lady’s Slipper Orchids (Paphiopedilum) collected in the Bogor Botanical Garden, Indonesia. Caryologia, 77(1), 57–64. https://doi.org/10.36253/caryologia-2232

Issue

Vol. 77 No. 1 (2024)

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Copyright (c) 2024 Titien Ng. Praptosuwiryo, R. Vitri Garvita, Elizabeth Handini, Izu Andry Fijridiyanto, Joko Ridho Witono

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