The effect of TiO2 and SiO2 nanoparticles and salinity stress on expression of genes involved in parthenolide biosynthesis in Feverfew (Tanacetum parthenium L.)

  • Mahshid Khajavi
  • Mahdi Rahaie
  • Asa Ebrahimi
Keywords: Tanacetum parthenium L., Nanoparticle, SiO2, TiO2, Salinity stress, Gene expression analysis

Abstract

Medicinal plants can produce various chemical compounds as secondary metabolites that have benefit to human. Feverfew (Tanacetum parthenium L.) is a medicinal plant belongs to the Asteraceae family. This plant due to have parthenolide compounds has attracted much attention for medicinal value and pharmacological activity. Due to the economic importance of the plant metabolite in cancer and migraine treatment, application of approaches for increasing the metabolite was the objective of this study. For this purpose, after cultivation in greenhouse, plants were treated with TiO2 and SiO2 nanoparticles and salinity stress at different times and concentrations. Real Time PCR used to evaluate the expression of TpGAS, COST and TpCarS genes which involved in secondary metabolites biosynthesis pathway (parthenolide and β-caryophyllen). It was found, SiO2 NPs can increase the expression of TpCarS, COST and TpGAS in the concentration of 25mM with increasing time from 6 to 24h. In this concentration (25mM), TiO2 treatment, up-regulated the COST and TpGAS in contrast, down-regulated the TpCarS with increasing time from 6 to 24h. Salinity treatment affected the expression of all three genes, so that with increasing time, the expression of all three genes was elevated. In conclusion, according to above and HPLC results, it was shown the nanoparticles and salinity treatments can increase parthenolide synthesis in whole plant of Feverfew and then they can be used as elicitor for more production of the metabolite.

Author Biographies

Mahshid Khajavi

Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Science and Research Branch of Islamic Azad university, Tehran, Iran

Mahdi Rahaie

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

Asa Ebrahimi

Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Science and Research Branch of Islamic Azad university, Tehran, Iran

Published
2019-05-10
How to Cite
Khajavi, M., Rahaie, M., & Ebrahimi, A. (2019). The effect of TiO2 and SiO2 nanoparticles and salinity stress on expression of genes involved in parthenolide biosynthesis in Feverfew (Tanacetum parthenium L.). Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 72(1), 3-14. https://doi.org/10.13128/cayologia-246
Section
Articles