Chromosomes damage by sewage water studies in the Allium cepa L. and Zea mays L.
DOI:
https://doi.org/10.36253/caryologia-1067Keywords:
sewage water, mitotic index, indian standard, heavy metal, cytotoxic, ecosystemAbstract
The effect of sewage water sample of the three locations Khardah (22.7003° N, 88.3753° E), Titagarh (22.7383° N, 88.3737° E), Madhyamgram (22.6924° N, 88.4653° E), and Control (distilled H2O) in the district of North 24 Parganas (22.6168° N, 88.4029° E), West Bengal, India on the damage of chromosomes in the onion (Allium cepa L.) and maize plant (Zea mays L.) were investigated by employing mitotic chromosomal aberration assay. Physiochemical analysis of sewage water samples showed the pH is 5.10-5.30 in nature. Few heavy elements: Fe, Mn and Zn in the sample from Khardah (22.7003° N, 88.3753° E) sewage water exceeded the Indian Standard 10500:2012 and WHO’s (2006) permissible limits. Whereas Cl, Cu, Pb, Cr, and Cd are more or less within limit of the standard condition. The obtained data exhibited a decline in reproductive capacity of cells and the occurrence of deviation from the normal mitotic cell division. The mitotic index (MI) decreased significantly (p < 0.05) in both the cases and is given as Control (57.03 %) > Madhyamgram (41.70 %) > Titagarh (33.85 %) > Khardah (31.57 %) in Allium cepa L. and Control (49.33 %) > Titagarh (21.45 %) > Madhyamgram (26.47 %) > Khardah (24.05 %) in Zea mays L. The chromosomal aberrations (CAs): Karyorrhexis, Karyolysis, Fragments, Lagging chromosome, Anaphase bridges are present in significant amount in the crops treated with sewage water sample than the one with control condition. Heavy metals act as pollutants in the sewage water sample which has cytotoxic effect on cells, threat to water ecosystem and human health.
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References
Anderson O. 1985. Evaluation of the spindle inhibition effect of Ni2+quantitation of
chromosomal super condensation. Res Commun Chem Pathol Phatmacol. 50:379-384.
Ayansina AS, Olubukola BO. 2017. A new strategy for heavy metal polluted environments:
A Review of Microbial Bio-sorbents. Int J Environ Res Public Health. 14(1):94.
Babish JG, Johnson BE, Lisk DJ.1983. Mutagenicity of municipal sewage sludge of American
cities. Environ Sci Technol.17:272-277.
Blevins RD, Brennan LA.1990. Fate of mutagenic activity during conventional treatment of
Municipal waste water sludge. Arch Environ Toxicol. 19:657-664.
Brown KW, Thomas JC, Donelly KC. 1991. Bacterial mutagenicity of municipal sewage sludge.
J Environ Sci Health. 26:359-413.
Chauhan LKS, Saxena PN, Sundararaman V, Gupta SK. 1998. Diuron induced cytological and
ultrastructural alterations in the root meristem cells of Allium cepa. Pestic Biochem Physiol. 62:152-163.
Egito LCM, Medeiros MG, De-Medeiros SRB, Agnes–Lima LF. 2007. Cytogenetic and
Genotoxic potential of surface water from the Pitimbu river, northeastern/RN Brasil. Genet Mol Biol. 30:1-15.
Fashola MO, Ngole-Jeme VM, Babalola OO. 2016. Heavy metal pollution from gold mines:
Environmental effects and bacterial strategies for resistance. Int J Environ Res Public Health.13(11): 1047.
Fiskesjo G.1997. Allium test for screening chemicals: evaluation of cytological parameters. In:
Plants for Environmental Studies, Lewis Publishers, Boca Raton, New York; p.307-333.
Fiskesjo G. 1985. The Allium test as a standard in environmental monitoring. Hereditas.102:99-
112.
Godet F, Babut M, Burnel D, Veber AM, Vasseur P. 1993. The Genotoxicity of iron and
chromium in Electro planting effluents. Mutat Res. 370:19-28.
Gomez KA, Gomez AA. 1984. Statistical procedures for agricultural research. Willey, New
York.
Gomurgen AN. 2005. Cytological effect of the potassium metabisulphite and potassium nitrate
food preservative on root tip of Allium cepa L. Cytologia. 70:119-128.
Grant WF.1982. Chromosome aberration assays in Allium. A report of the United States
environmental protection agency gen toxicity program. Mutat Res. 99:273-291.
Ivanova E, Staikova TA, Velcheva L. 2005. Cytogenetic testing of heavy metals and cyanide
contaminated river water in a mining region of southwest Bulgaria. J Cell and Mole Bio. 4:99-106.
Ivanova E, Staikova T, Velcheva I. 2002. Mutagenic effect of water polluted with heavy metals
and cyanides on Pisum sativum plant In vivo. J Balkan Eco. 5(3):307-310.
Moraes D, Jordao B. 2001. Evaluation of the genotoxic potential of municipal waste water
discharged into the Paraguay River during periods of flood and drought. Environ Toxicol. 16:113-116.
Mumma RO, Rashid KA, Raupach DC, Shane BS, Scarlet- Kranz JM. 1988. Mutagens, toxicants
and other constituents in small cities sludges in New York State. Arch Environ Contam Toxicol. 17:657- 663.
Nagajyoti P, Lee K, Sreekanth T. 2010. Heavy metals, occurrence and toxicity for plants: A
review. Environ Chem Lett. 8:199-216.
Olorunfemi Dl, Olorunfemi OP, Agbozu IE. 2014. Genotoxicity assessment of contaminated
drinking water sources in a rural community in Edo State of Nigeria. J Geosc Env Protec. 2:52-59.
Rank J, Nielsen M. 1998. Genotoxicity testing of wastewater sludge using the Allium cepa
anaphase-telophase chromosome aberration assay. Mutat Res. 418:113-119.
Rank J. 2003.The method of Allium anaphase-telophase chromosome aberration assay.
Ekologija.1:38-42.
Ray M, Saha R.1992. Cytological effects of industrial effluents on root meristem cells of Allium
sativum L.: Carbon black and chemical factory effluents. In: Perspective in Cytology and Genetics, eds. Khanna GK, Roy SC.7: 1167-1175.
Schneiderman MH, Dewey WC, Highfield DP.1971. Inhibition of DNA synthesis in
synchronized Chinise hamster cells treated in G1 with Cyclohexamid. Exp Cell Res.
67:147-155.
Sharma AK, Sharma A. 1980. Chromosome Techniques, Theory and Practice, Third edition,
Butterworth & Co. (Publisher) Ltd. London.
Smaka-Kinkl V, Stegnar P, Lovka M, Toman M. 1996. The evaluation of waste, surface and
ground water quality using the Allium test procedure. Mutat Res. 368:171-179.
Turkoglu S. 2007. Genotoxicity of five food preservatives tested on root tips of Allium cepa L.
Mutat Res. 626 (1-2): 4-14.
Zang Y, Yang X.1994.The toxic effects of cadmium on cell division and chromosomal
morphology of Hordeum vulgare. Mutat Res. 312:121-126.
Lee YW, Klein CB, Kargacin B, Salnikow K, Kitahara J, Dowjat K, Zhitkovich A, Christie NT,
Costa M. 1995. Carcinogenic nickel silence gene expression by chromatin condensation
and DNA methylation: a new model for epigenetic carcinogens. Mol cell Biol.15: 2547-
2557.
White P, Rasmussen JB.1998. The genotoxic hazard of domestic wastes in surface waters. Mutat Res. 410:223-236.
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