Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa

Authors

  • Kun Zhu Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, College of Life Science, Agriculture and Forestry, Qiqihar University, Qiqihar Heilongjiang Province
  • Lijie Liu Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, College of Life Science, Agriculture and Forestry, Qiqihar University, Qiqihar Heilongjiang Province
  • Shanshan Li Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, College of Life Science, Agriculture and Forestry, Qiqihar University, Qiqihar Heilongjiang Province
  • Bo Li Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, College of Life Science, Agriculture and Forestry, Qiqihar University, Qiqihar Heilongjiang Province
  • Majid Khayatnezhad Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil
  • Abdul Shakoor College of Environment and Planning, Henan University, Kaifeng, 475004, Henan

DOI:

https://doi.org/10.36253/caryologia-958

Keywords:

Allochrusa, ISSR–Analysis, network, population structure, species delimitation

Abstract

The Caryophyllaceae family is complex. Several attempts have been carried out in the past to study Caryophyllaceae members. This study mainly focused on Allochrusa Bunge to determine its genetic structure and used ISSR markers, ITS, and rps16 data to classify and differentiate Allochrusa species. We collected 122 Allochrusa specimens. Our analysis included morphological and molecular method approaches. Morphometry analysis indicated that floral characters could assist in the identification of Allochrusa species. A. persica (Boiss.) Boiss. and A. versicolor Fisch. & C.A.Mey. showed affinity to each other. A. bungei Boiss. formed a separate group. Analysis of molecular variance showed significant genetic differentiation in Allochrusa (p= 0.001). The majority of genetic variation was among the Allochrusa population. We recorded minimum gene flow (Nm=0.176) between Allochrusa species. Besides this, isolation by distance occurs in Allochrusa members, as shown in the Mantel test result (r = 0.01, p = 0.0002). STRUCTURE analysis revealed three genetic groups. It is evident that A. persica, A. versicolor, and A. bungei differ genetically from each other. Our current findings have implications in plant systematics and biodiversity management.

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Published

2021-10-08

How to Cite

Zhu, K., Liu, L., Li, S., Li, B., Khayatnezhad, M., & Shakoor, A. (2021). Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa. Caryologia, 74(2), 121-130. https://doi.org/10.36253/caryologia-958

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Section

Articles