Chemical composition, antioxidant and cytogenotoxic effects of Ligularia sibirica (L.) Cass. roots and rhizomes extracts

  • Nicoleta Anca Şuţan University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Andreea Natalia Matei University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Eliza Oprea University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Blvd. Regina Elisabeta, No. 4-12, 030018, Bucharest, Romania https://orcid.org/0000-0001-7051-4136
  • Victorița Tecuceanu Organic Chemistry Centre of the Romanian Academy “Costin D. Nenitescu”, 202B Splaiul Independentei, 78100 Bucharest, Romania
  • Lavinia Diana Tătaru University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Sorin Georgian Moga University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Denisa Stefania Manolescu University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Carmen Mihaela Topală University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania https://orcid.org/0000-0002-9117-4983
Keywords: antioxidant activity, phenols, SFE extraction, cytogenetic effects, Ligularia sibirica, HPLC

Abstract

Through time, in the traditional medicine Ligularia genus has been used as a remedy to cure several diseases and affections. The paper represents an essential step in offering more information about the antioxidant activity, chemical composition and cytogenetic activity of L. sibirica (L.) Cass. rhizomes and roots extracts. The antioxidant activity of the extracts has been achieved by analyzing the total phenolic content, total flavonoids, the organic chemical compound and trolox equivalent antioxidant capacity and their major polyphenolic constituents were quantified by liquid chromatography electrospray ionization-tandem mass spectrometry. The extracts were obtained by the Supercritical Fluid Extraction (SFE-CO2) technique, SFE-CO2 extraction with co-solvent and absolute ethanol extraction. The best results for the antioxidant activity have been fulfilled through the last two techniques. High Performance Liquid Cromatography (HPLC) has been applied in order to identify and quantify selected phenolic acids and flavonoids in the ethanolic extracts of L. sibirica (L.) Cass. The cytogenotoxic effects of the extracts completed the present study, with a furtherance of antiproliferative potential highlighted by the statmokinetic effect and an additional genoprotective effect.

Author Biographies

Andreea Natalia Matei, University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania

University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences

Eliza Oprea, University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Blvd. Regina Elisabeta, No. 4-12, 030018, Bucharest, Romania

Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest

Victorița Tecuceanu, Organic Chemistry Centre of the Romanian Academy “Costin D. Nenitescu”, 202B Splaiul Independentei, 78100 Bucharest, Romania

Organic Chemistry Centre of the Romanian Academy “Costin D. Nenitescu”

Lavinia Diana Tătaru, University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania

University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences

Carmen Mihaela Topală, University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania

University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences

Published
2020-03-13
How to Cite
Şuţan, N. A., Matei, A. N., Oprea, E., Tecuceanu, V., Tătaru, L. D., Moga, S. G., Manolescu, D. S., & Topală, C. M. (2020). Chemical composition, antioxidant and cytogenotoxic effects of Ligularia sibirica (L.) Cass. roots and rhizomes extracts. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 73(1). https://doi.org/10.13128/caryologia-116
Section
Articles