Phytochemical analysis and in vitro assessment of Polystichum setiferum extracts for their cytotoxic and antimicrobial activities

  • Nicoleta Anca Şuţan University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Irina Fierăscu The National Institute for Chemistry and Petrochemistry – ICECHIM, 202 Spl. Independentei, 060021, Bucharest, Romania
  • Radu Fierăscu The National Institute for Chemistry and Petrochemistry – ICECHIM, 202 Spl. Independentei, 060021, Bucharest, Romania
  • Deliu Ionica University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
  • Liliana Cristina Soare University of Piteşti, Faculty of Sciences, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Romania
Keywords: Polystichum, extracts, FTIR, EDXRF, UV-VIS, cytogenotoxicity, antimicrobial activity

Abstract

Ferns are traditionally used by some nations to treat rheumatism, lungs, gynecology, blood and digestion dysfunctions, and several others illnesses. The present study evaluates the bioactivity of methanol and ethanol extracts from Polystichum setiferum (Forssk.) Moore ex Woyn. in an Allium cepa test and disk diffusion test. In the Allium cepa test the methanol and ethanol extracts induced a significant time-related increase in the mitotic index. The tested extracts were non-mutagenic by used assay, with no occurrence either the structural or numerical aberrations detected. The extracts were also evaluated in terms of trace elements (by EDXRF) and qualitative composition (by UV-VIS, FTIR and total phenolic content). In the disk diffusion test, methanol extracts from leaves determined a small inhibition of bacterial growth for Enterobacter cloacae and Citrobacter freundii strains relatively to control sample (methanol). The ethanol extracts were more efficient, the diameter of inhibition growth zones measured from 7 to 10 mm, the most affected strain was Chryseobacterium meningosepticum.

Published
2019-12-05
How to Cite
Şuţan, N. A., Fierăscu, I., Fierăscu, R., Ionica, D., & Soare, L. C. (2019). Phytochemical analysis and in vitro assessment of Polystichum setiferum extracts for their cytotoxic and antimicrobial activities. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 72(2), 53-61. https://doi.org/10.13128/cayologia-255
Section
Articles

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