Biogenic synthesis of noble metal nanoparticles using Melissa officinalis L. and Salvia officinalis L. extracts and evaluation of their biosafety potential

Authors

  • Denisa Manolescu Regional Research and Development Center for Innovative Materials, Products and Processes from Automotive Industry, University of Pitesti; Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest https://orcid.org/0000-0002-9777-6782
  • Georgiana Uță Regional Research and Development Center for Innovative Materials, Products and Processes from Automotive Industry, University of Pitesti; Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest https://orcid.org/0000-0002-7442-7109
  • Anca Șuțan Department of Natural Sciences, Faculty of Science, Physical Education and Informatics, University of Pitesti https://orcid.org/0000-0001-7459-628X
  • Cătălin Ducu Regional Research and Development Center for Innovative Materials, Products and Processes from Automotive Industry, University of Pitesti https://orcid.org/0000-0002-5235-9678
  • Alin Din Regional Research and Development Center for Innovative Materials, Products and Processes from Automotive Industry, University of Pitesti
  • Sorin Moga Regional Research and Development Center for Innovative Materials, Products and Processes from Automotive Industry, University of Pitesti https://orcid.org/0000-0001-9620-4283
  • Denis Negrea Regional Research and Development Center for Innovative Materials, Products and Processes from Automotive Industry, University of Pitesti https://orcid.org/0000-0001-7525-1056
  • Andrei Biță Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova https://orcid.org/0000-0003-4715-1423
  • Ludovic Bejenaru Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova
  • Cornelia Bejenaru Department of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova
  • Speranța Avram Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest https://orcid.org/0000-0002-4245-2502

DOI:

https://doi.org/10.36253/caryologia-1774

Keywords:

metal nanoparticles, antioxidant, cytotoxicity, lemon balm, sage

Abstract

In this study we targeted the noble metal nanoparticles (MNPs) biogenic synthesis capacity of two medicinal species with therapeutic potential, namely Melissa officinalis L. (lemon balm) and Salvia officinalis L. (sage), cultivated in Romania. Plant material was extracted by maceration, microwave assisted extraction (MAE) and ultrasound assisted extraction (UAE). Bright field scanning transmission electron microscopy and energy dispersive X-ray spectroscopy (BFSTEM-EDS) techniques were used in order to investigate particles shape, dispersion and chemical elemental analysis. The total polyphenol content for both simple extracts and nanostructured mixtures was determined using the Folin-Ciocalteu method and antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Identification and quantification of secondary metabolites of M. officinalis and S. officinalis were performed by ultra-high performance liquid chromatography (UHPLC). The Allium assay was used to evaluate the potential cytogenotoxic activity, for both simple and nanostructured phytochemical complexes, in the case of S. officinalis L. species being performed for the first time. Spherical shaped MNPs with diameters of about 20 nm were biosynthesised in lemon balm extracts. Larger AuNPs were phytosynthesized in sage extract obtained by UAE. Compared to the simple extracts, the antioxidant capacity as well as the amount of total polyphenols in the nanostructured extracts decreased, substantiating the involvement of bioorganic material in the reduction of metal ions. Low frequency of chromosomal aberrations corresponding to crude extracts and extracts supplemented with MNPs, suggest the cytoprotective, antigenotoxic, and safe use of these plant species as potential therapeutic forms in various diseases.

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Published

2023-02-12

How to Cite

Manolescu, D., Uță, G., Șuțan, A., Ducu, C., Din, A., Moga, S., Negrea, D., Biță, A., Bejenaru, L., Bejenaru, C., & Avram, S. (2023). Biogenic synthesis of noble metal nanoparticles using Melissa officinalis L. and Salvia officinalis L. extracts and evaluation of their biosafety potential. Caryologia, 75(3), 65–83. https://doi.org/10.36253/caryologia-1774

Issue

Vol. 75 No. 3 (2022)

Section

Articles

License

Copyright (c) 2022 Georgiana U??, Denisa Manolescu, Anca ?u?an, C?t?lin Ducu, Alin Din, Sorin Moga, Denis Negrea, Andrei Bi??, Ludovic Bejenaru, Cornelia Bejenaru, Speran?a Avram

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