Anti-haemolytic and cytogenotoxic potential of aqueous leaf extract of Annona muricata (L.) and its bio-fabricated silver nanoparticles

Authors

DOI:

https://doi.org/10.36253/caryologia-1353

Keywords:

green synthesis, anti-haemolytic, Annona muricata, Allium cepa, silver nanoparticles, cytogenotoxicity

Abstract

Nanotechnology is widely gaining worldwide application in biology and medicine because of its proven efficacy. Annona muricata contains bioactive phytochemicals with an inherent ability to bio-fabricate metal ions nanoparticles (NPs). Annona muricata aqueous leaf extract and its green bio-fabricated silver nanoparticles were evaluated on red blood cells (RBC) for anti-haemolytic activity and cytogenotoxicity on Allium cepa cells. The effects of A. muricata extract (Am-E) and its biofabricated silver nanoparticles (Am-AgNPs) were observed at 0.7, 7.0 and 70.0 µg/ml on H2O2-induced haemolysis in RBC and cyclophosphamide-induced cytogenotoxicity on A. cepa cells. Results showed significant and concentration dependent anti-haemolytic activity of Am-E relative to Am-AgNPs. Significant (P<0.05) reduction of mitotic index was observed in the groups treated with Am-AgNPs compared with Am-E, which indicates cytotoxic effect of the nanoparticles. The Am-E protected A. cepa meristem root cells from cyclophosphamide-induced mitotic repression better than Am-AgNPs. Different degree of chromosomal abnormalities such as chromosome-bridge, sticky chromosome, and c-mitosis were observed in all the treatment groups with chromosome-bridge and sticky chromosome being prominent. This study revealed stronger anti-haemolytic efficacy of Am-E at higher concentrations compared with Am-AgNPs. Chromosomal abnormalities observed in this study suggest greater chromosomal instability as influenced by the nanoparticles compared with the extract on onion cells. The protective effect of the extract against cyclophosphamide-induced chromosomal aberrations may be an indication of its potential as an anti-genotoxic agent. 

Downloads

Download data is not yet available.

Downloads

Published

2022-07-07

How to Cite

Badmus, J. A., Oyemomi, S. A., Fatoki, J. O., Yekeen, T. A. ., Adedosu, O. T. ., Adegbola, P. I., Azeez, M. A. ., Adebayo, E. A., & Lateef, A. . (2022). Anti-haemolytic and cytogenotoxic potential of aqueous leaf extract of Annona muricata (L.) and its bio-fabricated silver nanoparticles. Caryologia, 75(1), 3-13. https://doi.org/10.36253/caryologia-1353

Issue

Section

Articles