Classical chromosome features and microsatellites repeat in Gekko petricolus (Reptilia, Gekkonidae) from Thailand

Authors

  • Weera Thongnetr Walai Rukhavej Botanical Research institute, Mahasarakham University, Kantharawichai, Maha Sarakham 44150
  • Surachest Aiumsumang Biology program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000
  • Alongklod Tanomtong Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002
  • Sumalee Phimphan Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002

DOI:

https://doi.org/10.36253/caryologia-1544

Keywords:

Karyotype, Gekko petricolus, Ag-NOR, FISH microsatellite

Abstract

The objectives of this study were to examine size, shape, diploid number (2n), fundamental number (NF), NORs region, and distribution of microsatellite by using Fluorescence in situ hybridization technique (FISH) and to establish the karyotype and standard idiogram of sandstone geckos (Gekko petricolus Taylor, 1962). Sandstone gecko distributed in the sandstone mountains in Laos, Cambodia, and Thailand. Five male and five female specimens were collected  from Ubon Ratchathani and Mukdahan provinces, Thailand. The metaphase cells were directly prepared from the bone marrow cells. Chromosomes were stained by conventional staining, NORs-banding and FISH techniques. The results found that the diploid number was 38 chromosomes. The fundamental number was 54. The karyotype composed of 4 large metacentric, 4 large acrocentric, 2 large telocentric, 4 medium acrocentric, 2 medium telocentric, 2 small submetacentric, 2 small acrocentric and 18 small telocentric chromosomes. No morphological difference was identified between sex chromosomes of male and female specimens. The NORs appeared to telomere of the long arm of chromosome pair 17. The study displayed that the distribution of microsatellite using (CA)15 and (GAA)10 probes distributed throughout the genome. However, (CA)15 sequences concentrated in the telomere. The karyotype formula G. petricolus is as follow: 2n (38) = Lm4+La4+Lt2+Ma4+Mt2+Ssm2+Sa2+St18.

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Published

2022-09-21

How to Cite

Thongnetr, W., Aiumsumang, S. ., Tanomtong, A., & Phimphan, S. (2022). Classical chromosome features and microsatellites repeat in Gekko petricolus (Reptilia, Gekkonidae) from Thailand. Caryologia, 75(2), 81–88. https://doi.org/10.36253/caryologia-1544

Issue

Vol. 75 No. 2 (2022)

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Copyright (c) 2022 Weera Thongnetr, Surachest Aiumsumang, Alongklod Tanomtong, Sumalee Phimphan

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