Comparative chromosome mapping of repetitive DNA in four minnow fishes (Cyprinidae, Cypriniformes)

Authors

  • Surachest Aiumsumang Biology Program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000
  • Patcharaporn Chaiyasan Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002
  • Kan Khoomsab Education Science Program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000
  • Weerayuth Supiwong Applied Science Program, Faculty of Interdisciplinary Studies, Khon Kaen University, Nong Khai Campus, Muang, Nong Khai 43000
  • Alongklod Tanomtong Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002
  • Sumalee Phimphan Biology Program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000

DOI:

https://doi.org/10.36253/caryologia-1523

Keywords:

Cyprinidae, Danioninae, FISH, microsatellite

Abstract

The present study focused on the repetitive DNA of the chromosome in four minnow fishes from the genera Danio Hamilton, 1822, Devario Heckel, 1843 and Rasbora Bleeker, 1859. Chromosomes were analysed using fluorescence in situ hybridization (FISH) with microsatellite probes including (CA)15, (CAC)10, (CGG)10, (GC)15 and (TA)15 staining. All species retained the diploid chromosome number 2n = 50 in male and female. The microsatellite sequences were mapped in the chromosomes of Danio albolineatus (Blyth, 1860), Devario regina (Fowler, 1934), Rasbora aurotaenia Tirant, 1885 and R. paviana Tirant, 1885. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the telomeric region and the whole genome, which suggests that sequences contribute to chromosome structure and may have played a role in the relationship of this fish group. The comparative genome mapping data presented here provide novel information on the structure and organisation of the repetitive DNA region of the minnow’s genome and contribute to a better understanding of the genomes of these minnows.

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References

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2022-09-21

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Aiumsumang, S. ., Chaiyasan, P., Khoomsab, K., Supiwong, W., Tanomtong, A., & Phimphan, S. (2022). Comparative chromosome mapping of repetitive DNA in four minnow fishes (Cyprinidae, Cypriniformes). Caryologia, 75(2), 71–80. https://doi.org/10.36253/caryologia-1523

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Vol. 75 No. 2 (2022)

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