The Chromosome counts of some species of wetland plants from Northwest Iran

chromosome number


  • Saeedeh Sadat Mirzadeh Vaghefi Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), P. O. Box 13185-116, Tehran
  • Adel Jalili Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), P. O. Box 13185-116, Tehran



chromosome number, wetland, ideogram, Azerbaijan


Wetlands scatter as microclimates in mountain areas of Iran. In this investigation, the chromosome number of species and populations from Azerbaijan provinces were studied. After seed germination and root fixation, meristem cells were stained and photographs were taken for cells in the metaphase stage by light microscope. Data were analyzed by Micromeasure and excel softwares. As many as 28 populations of 24 species were studied. The chromosome numbers of two species (viz. Ranunculus kotschyi) were reported for the first time. 11 populations in 9 species were reported for the first time from Iranian populations (viz. Alisma plantago-aquatica, Prunella vulgaris, Scrophularia umbrosa). The range of haploid chromosome numbers is between n=6 and n=21. Ideograms were depicted for each species.


Download data is not yet available.


Agapova, N.D., 1981. A comparative karyological study of some Ranunculus species from the European part of the. USSR. Bot. Zhurn. SSSR 66, 483–492.

Albach, D., Delgado, L., Weiss-schneeweiss, H., 2009. Number Annals of the Missouri Botanical Garden 95, 543–566. doi:10.3417/2006094

Albach, D.C., Martínez-Ortega, M.M., Delgado, L., Weiss-Schneeweiss, H., Özgökce, F., Fischer, M.A., 2008. Chromosome numbers in Veroniceae (Plantaginaceae): Review and several new counts. Ann. Missouri Bot. Gard. 95, 543–566. doi:10.3417/2006094

Alinejad, K., Ramezani, E., Naqinezhad, A., Djamali, M., 2017. A preliminary floristic study of Neor high altitude wetland, Ardebil province, NW Iran. J. For. Res. Dev. 3, 221–235. doi:10.21859/ijnr-12029

Amosova, A. V, Zoshchuk, S.A., Rodionov, A. V, Ghukasyan, L., Samatadze, T.E., Punina, E.O., Loskutov, I.G., Yurkevich, O.Y., Muravenko, O. V, 2019. Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae / Poeae tribe complex ( Poaceae ) 9–12.

Aref Tabad, M., Jalilian, N., Maroofi, H., 2016. Study of flora, life form and chorology of plant Species in Zarivar Region of Marivan, Kurdistan. Taxon. Biosyst. 69–102.

Aryavand, A., 1977. In IOPB chromosome number reports LVIII. Taxon 26, 565–557.

Assadi, M., 1989. Flora of Iran (in Persian). Research Institute of Forests and Rangelands, Tehran.

Assadi, M., Maassoumi, A.A., Khatamsaz, M., Mozaffarian, V. (Eds. ., n.d. Flora of Iran. Research Institute of Forests and Rangelands Publications, Tehran. [in Persian], Tehran.

Astanova, S.B., 1999. Chromosome numbers of some representatives of the family Brassicaceae of the flora of Tadjikistan. Citologija 41, 1054–1055.


Baldwin, J.T., Speese, B.M., 1955. Chromosomes of Taxa of the Alismataceae in the Range of Gray’s Manual. Am. J. Bot. 42, 406. doi:10.2307/2438748

Baltisberger, M., 1991. IOPB chromosome data 3. Int. Organ. Pl. Biosyst.Newslett. 17, 5–7.

Baltisberger, M., Widmer, A., 2009. Chromosome numbers and karyotypes within the Ranunculus alpestris-group (Ranunculaceae). Org. Divers. Evol. 9, 232–243. doi:10.1016/j.ode.2009.03.003

Brutovska, R., Kusnirikuva, P., Bogyiova, E., Cellarova, E., 2000. Karyotype Analysis of Hypericum Perforatum L . Biol. Plant. 43, 133–136. doi:10.1023/A

Chehregani, A., Hajisadeghian, S., 2009. New chromosome counts in some species of Asteraceae from Iran 6–9. doi:10.1111/j.1756-1051.2009.00347.x

Ciccarelli, D., Garbari, F., Mártonfi, P., 2001. Mediterranean chromosome number reports 11 (1232). Fl. Medit. 11, 440–443.

Clifford Odets, 1954. The Flowering Peach 89.

Dahlgren, G., Cronberg, N., 1996. Species differentiation and relationships in Ranunculus subgenus Batrachium (Ranunculaceae) elucidated by isozyme electrophoresis. Symb. Bot. Upsal. 31, 91–104.

Dmitrieva, S.A., 1986. Chisla khromosom nekotorych vidov rastenij Berezinskogo Biosfernogo Zapovednika. Zapov. Beloruss. Issl. 10, 24–28.

Dobes, CH.; Hahn, B.; Morawetz, W., 1997. Chromosomenzahlen zur Gefäßpflanzen-Flora Österreichs. Linzer Biol. Beiträge 29, 5–43.

Dobes, C., Vitek, E., 2000. Documented Chromosome Number Checklist of Austrian Vascular Plants. Verlag des Naturhistorischen Museums Wien, Vienna.

Dzhus, M.A., Dmitrieva, S.A., 2001. Chromosome numbers in the species of the genus Veronica (Scrophulariaceae) from Byelorussia. Bot. Zhurn. (Moscow Leningrad) 86, 144–147.

F.S. Ghasemi, Jalili, A., Vaghefi, S.S.M., Ashrafi, & S., 2015. CHROMOSOME NUMBERS FOR RANUNCULUS DOLOSUS AND SILENE CONOIDEA FROM IRAN. Iran. J. Bot. 21, 61–63.

Gervais, C., Moreno, D., Drolet, E.T.A., 1993. Chromosomiques Et Reproduction ’ 2005–2008.


Ghaffari, S.M., 2006. New or rare chromosome counts of some angiosperm species from Iran II. Iran. J. Bot. 12, 81–86.

Ghaffari, S.M., 1999. Chromosome studies in the Iranian Asteraceae II. Iran. J. Bot. 8, 91–104.

Ghaffari, S.M., 1987. Chromosome counts of some Angiosperms from Iran II. Iran. J. Bot. 3, 183–188.

Ghaffari, S.M., 1986. Chromosome number reports. XCI. Taxon 35, 407.

Ghahremaninejad, F., Naqinezhad, A., Amirgholipour Kasmani, V., 2012. Plant diversity of five important wetlands of Babol Mazandaran province, Iran. Taxon. Biosyst. J. 4, 13–24.

Gorenflot, R., 1976. Le complexe polyploïde du phragmites australis (cav.) trin. ex Steud. (= P. communis trin.). Bull. la Soc. Bot. Fr. 123, 261–271. doi:10.1080/00378941.1976.10835694

Grau, J., 1979. The probable allopolyploid origin of Scrophularia auriculata and S. pseudoauriculata. Webbia 34, 497–499.

Hayirlioglu-Ayaz, H.I., 2011. NIH Public Access 2010, 149–157. doi:10.1007/s00606-009-0266-5.Chromosome

Hesamzadeh Hajazi, S.M., Ziaei Nasab, M., 2009. Color chromosome atlas of legumes collected in natural resources gene bank od Iran.

Hesamzadeh Hejazi, S.M., Rasuli, M., 2006. Cytogenetic study of some species of vetch genus (Vicia sp.) in Ira. Iran. J. Agric. Sci. 37, 213–225.

Iwatsubo, Y., Sugimoto, M., Suyama, C., Naruhashi., & N., 1998. Distribution and chromosome numbers of Triglochin maritimum L. s.l. (Juncaginaceae) in Chubu District, Japan. J. Phytogeogr. Taxon. 46, 195–199.

Jalili, A., Naqinezhad, A. r., Kamrani, A., 2014. Wetland Ecology (With an especial approach on wetland habitats of southern Alborz).268 pp.

Javurkova, V., 1979. In IOPB chromosome number reports LXIV. Taxon 28, 400–401.

Kalinka, A., Mifsud, S., Popiela, A., Achrem, M., 2014. Chromosome number of elatine gussonei (sommier) brullo (elatinaceae) and its distribution on the maltese islands. Acta Bot. Croat. 73, 267–273. doi:10.2478/botcro-2013-0022

Kaur, H., Mubarik, N., Kumari, S., Gupta, & R.C., 2011. IAPT/IOPB chromosome data 11. Taxon 60, 1220–1223.

Khanhasani, M., Jalili, A., Khodakarami, Y., Jalilian, N., 2021. Wetland flora of Kermanshah Province, Iran. Nov. Biol. Reper. 8, 154–172. doi:10.52547/nbr.8.2.154

Koull, K., Gohil, R.N., 1991. Cytogenetic Studies on Some Tribe Agrostideae , Kashmir Grasses . VIII Festuceae and Paniceae The present work is in continuation with the cytogenetic studies carried out on the fodder grasses of Jammu and Kashmir Himalayas ( Gohil and Koul 1988a , 1988b . Cytologia (Tokyo). 56, 437–452.

Krasnikov, A.A., 1991. Chromosome numbers in some species of vascular plants from Novosibirsk region. Bot. Zhurn. (Moscow Leningrad) 76, 476–479.

Krasnikov, A.A., Schaulo., D.N., 1990. Chromosome numbers in representatives of some families of vascular plants in the flora of the Novosibirsk region. II. Bot. Zhurn. (Moscow Leningrad) 75, 118–120.

Krasnikov, A.A., Schaulo, & D.N., 1990. Chromosome numbers in representatives of some families of vascular plants in the flora of the Novosibirsk region. II. Bot. Zhurn. (Moscow Leningrad) 75, 118–120.

Kuzmanov, B., 1993. Chromosome numbers of bulgarian angiosperms : An introduction to a chromosome atlas of the Bulgarian flora. Flora Mediterr. 3, 19–163.

Lessani, H., Chariat-panahi., S., 1979. In IOPB chromosome number reports LXV. Taxon 28, 635–636.

Levan, A., Fredga, K., Sandberg, A.A., 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52, 201–220.

Liehr, 0., 1916. Ist die angenommene Verwandtschaft der Helobiae und Polycarpiae auch in ihrer Cytologie zu erkennen? Cohns Beitr. Biol. Pflanz. 135–220.

Love, A., Love., D., 1942. Cyto-taxonomic studies on boreal plants. I. K. Fysiogr. Sallska. I. Lund Forhandl. 1–19.

Lövkvist, B., Hultgård., U.M., 1999. Chromosome numbers in south Swedish vascular plants. Opera Bot. 137, 1–42.

Lövkvist, B., Hultgård, U.M., 1999. Chromosome numbers in south Swedish vascular plants. Opera Bot. 137, 1–42.

Naqinezhad, A. r., 2012. Vegetation Database of Mountain Wetlands of Iran (Short Database Report). Biodivers. Ecol. 4, 306–306.

Ørgaard, M., Linde-laursen, I.B., 2014. Cytogenetics of Danish species of Barbarea ( Brassicaceae ): Chromocentres , chromosomes and rDNA sites Cytogenetics of Danish species of Barbarea ( Brassicaceae ): chromocentres , chromosomes and rDNA sites. doi:10.1111/j.2007.0018-0661.01987.x

Panahi., M., 1979. Le complexe polyploide du Phragmites australis (Cav.) Trin. ex Steud. (= P. communis Trin.) en Iran. Rev. Cytol. Biol. Vég., Bot. 2, 67–81.

Paule, J., Gregor, T., Schmidt, M., E-M, G., Dersch, G., Dressler, S., Wesche, K., Zizka, G., 2017. Chromosome numbers of the flora of Germany – a new online database of georeferenced chromosome counts and flow cytometric ploidy estimates. Plant Systematics and Evolution 303(8):, doi:10.1007/s00606-016-1362-y.

Petrova, A., Stanimirova, & P., 2001. chromosome number reports 11 (1248–1253). Fl. Medit. Mediterranean 11, 460–466.

Pogan, E., Jankun, A., Sawicka, Z., 1990. Further studies in chromosome numbers of Polish angiosperms, part 22. Acta Biol. Cracov., Ser. Bot. 31, 1–17.

Probatova, 2004. Chromosome numbers of some representatives of the flora of the Primorsky Territory. Bot. Zhurn. (Moscow Leningrad) 89, 1209–1217.

Probatova, N.S., 2000. Chromosome numbers in some plant species from the Razdolnaya (Suifun) river basin (Primorsky Territory). Bot. Zhurn. (Moscow Leningrad) 85, 102–107.

Ramsey, J., Ramsey, T., 2014. Ecological studies of polyploidy in the 100 years following its discovery. Phil Trans R Soc B. 369, 20130352. doi:10.1098/rstb.2013.0352.

Razaq, Z.A., Vahidy, A.A., Ali, S.I., 1994. Chromosome numbers in Compositae from Pakistan. Ann. Missouri Bot. Gard. 81, 800–808.

Rechinger KH., 2005. Flora Iranica. Akademisch Druck- U Verlagsanstalt, Graz.

Reeves, A., Tear, J., 2000. MicroMeasure for Windows, version 3.3. Free program distributed by the authors over the internet from.

Rotreklova, 2004. Chromosome numbers for some species of vascular plants from Europe. Biol. 59, 425–433.

Safikhani, K., Jalili, A., Jamzad, Z., 2018. Archive of SID Wetlands Flora of Hamedan Province ( Iran ) Archive of SID.

Sawicka, Z., 1991. In Further studies in chromosome numbers of Polish angiosperms, part 24. Acta Biol. Cracov., Ser. Bot. 33, 37–38.

Sharma OP., 2009. Plant taxonomy. 2nd ed. McGraw Hill Publishing Co., New Delhi.

Sheidai, M., Hamta, A., Mofldabadi, A.J., Noori-Daloir, and M.R., 1998. KARYOTYPIC STUDY OF TRIFOliUM SPECIES AND CULTIVARS IN IRAN. J. Sci. I.R.lran 9, 214–222.

Sheidai, M., Sodabeh Jafari, And, P.T., Keshavarz, M., 2009. Cytomixis and Unreduced Pollen Grain Formation in Alopecurus L. and Catbrosa Beauv. ( Poaceae ) 74, 31–41.

States, U., Protection, E., (EPA), A., 2001. Types of Wetlands.

Stebbins, G.L., 1971. Chromosome evolution in higher plants. Edward Arnold Publisher, London.

Uchiyama, H., 1989. Karyomorphological studies on some taxa of the Helobiae. J. Sci. Hiroshima Univ.Ser. B, Div. 2, Bot. 22, 271–352.

Vitek, E., M. Kiehn, K. Pascher, F. Starlinger, J. Greimler, U. Stocker, S. Lehner, P. Beinhofer & A. Blaha.Vitek, E., M. Kiehn, K. Pascher, F. Starlinger, J. Greimler, U. Stocker, S. Lehner, P.B.& A.B., 1992. Beiträge zur Flora von Österreich—weitere Chromosomenzählungen. Verh. Zool.-Bot. Ges. Wien 129, 215–226.

Wood, T.E., Takebayashi, N., Barker, M.S., Mayrose, I., Greenspoon, P.B., Rieseberg, L.H., 2009. The frequency of polyploid speciation in vascular plants. Proc. Natl. Acad. Sci. U. S. A. 106, 13875–13879. doi:10.1073/pnas.0811575106

Wulff, H.D., 1939. Chromosomenstudien an der schleswigholsteinischen Angiospermen-Flora. III. Ber. Deutsch. Bot. Ges. 84–91.

Zhang, Z. p., Wu, L. h., Kang, Y. f., 1993. Analyses of karyotypes in six forage grass species of Leguminosae. Bot. Res 1, 60–61.




How to Cite

Mirzadeh Vaghefi, S. S., & Jalili, A. (2023). The Chromosome counts of some species of wetland plants from Northwest Iran: chromosome number. Caryologia, 75(4).