Classical chromosome features and microsatellites repeat in Gekko petricolus (Reptilia, Gekkonidae) from Thailand

Authors

  • Weera Thongnetr Walai Rukhavej Botanical Research institute, Mahasarakham University, Kantharawichai, Maha Sarakham 44150
  • Surachest Aiumsumang Biology program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000
  • Alongklod Tanomtong Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002
  • Sumalee Phimphan Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002

DOI:

https://doi.org/10.36253/caryologia-1544

Keywords:

Karyotype, Gekko petricolus, Ag-NOR, FISH microsatellite

Abstract

The objectives of this study were to examine size, shape, diploid number (2n), fundamental number (NF), NORs region, and distribution of microsatellite by using Fluorescence in situ hybridization technique (FISH) and to establish the karyotype and standard idiogram of sandstone geckos (Gekko petricolus Taylor, 1962). Sandstone gecko distributed in the sandstone mountains in Laos, Cambodia, and Thailand. Five male and five female specimens were collected  from Ubon Ratchathani and Mukdahan provinces, Thailand. The metaphase cells were directly prepared from the bone marrow cells. Chromosomes were stained by conventional staining, NORs-banding and FISH techniques. The results found that the diploid number was 38 chromosomes. The fundamental number was 54. The karyotype composed of 4 large metacentric, 4 large acrocentric, 2 large telocentric, 4 medium acrocentric, 2 medium telocentric, 2 small submetacentric, 2 small acrocentric and 18 small telocentric chromosomes. No morphological difference was identified between sex chromosomes of male and female specimens. The NORs appeared to telomere of the long arm of chromosome pair 17. The study displayed that the distribution of microsatellite using (CA)15 and (GAA)10 probes distributed throughout the genome. However, (CA)15 sequences concentrated in the telomere. The karyotype formula G. petricolus is as follow: 2n (38) = Lm4+La4+Lt2+Ma4+Mt2+Ssm2+Sa2+St18.

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References

Aprea G, Odierna G, Capriglione T, Caputo V, Morescalchi A, Olmo E. 1996. Heterochromatin and NOR distribution in the chromosomes of six gekkonid species of the genus Phelsuma (Squamata: Gekkonidae). African Zoology 110 (5), 341–349.

Chen J, Peng X, Yu D. 1986. Studies on the karyotype of three species of the genus Gekko. Acta Herpetologica Sinica 5, 24–29.

De Smet WHO. 1981. Description of the orcein stained kariotypes of 27 lizard species (Lacertilia: Reptilia) belonging to the familias Iguanidae, Agamidae, hamaeleontidae and Gekkonidae (Ascalabota). Acta zoologica et pathologica Antverpiensia 76: 35–72.

Gorman, G.C. 1973. Cytotaxonomy and vertebrate evolution. New York: Academic Press. The chromosomes of the Reptilia, a cytotaxonomic interpretation; p. 349–424.

Howell WM, Black DA. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.

Kawai A, Ishijima J, Nishida C, Kosaka A, Ota H, Kohno S, Matsuda Y. 2009. The ZW sex chromosomes of Gekko hokouensis (Gekkonidae, Squamata) represent highly conservedhomology with those of avian species. Chromosoma 118: 43–51.

King, M. 1984. Karyotypic evolution in Gehyra (Gekkonidae: Reptilia) IV, chromosome change and speciation. Genetica 64(2): 101–114.

Koubova M, Pokorna MJ, Rovatsos M, Farkačova K, Altmanova M, Kratochvil L. 2014. Sex determination in Madagascar geckos of the genus Paroedura (Squamata: Gekkonidae): are differentiated sex chromosomes indeed so evolutionary stable?. Chromosome Research 22: 441–452.

Kluge AG. 2001. Gekkotan lizard taxonomy. Hamadryad 26: 1–209.

Kubat Z, Hobza R, Vyskot B, Kejnovsky E. 2008. Microsatellite accumulation in the Y chromosome of Silene latifolia. Genome 51: 350–356.

Li HH, Tang ZJ, Yu TL, Chen M, Huang CM. 1996. Resources and protect of Gekko gecko in Guangxi. J. Guangxi Normal Univ. (Natural Science) 14: 62–66.

Luu QV, Calame T, Nguyen T, Le DE. 2015. Morphological and molecular review of the Gekko diversity of Laos with descriptions of three new species. Zootaxa 3986 (3): 279–306.

Lau MW, Ota H, Bogadek A. 1997. Chromosomes polymorphismand karyotype of Gekko chinensis (Tokaynidae: Reptilia) from Hong Kong. Journal of Herpetology 31: 137–139.

Moritz C. 1984. The evolution of a highly variable sex chromosome in Gehyra purpurascens (Gekkonidae). Chromosoma 90: 111–119.

Ngo TV, Bauer AM, Wood PL, Grismer JL. 2009. A new species of Gekko Laurenti, 1768 (Squamata: Gekkonidae) from Dong Nai Province, Southeastern Vietnam. Zootaxa 2238: 33–42.

Ngo TV, Gamble T. 2011. Gekko canaensis sp. nov. (Squamata: Gekkonidae), a new gecko from Southern Vietnam. Zootaxa 2890: 53–64.

Ota H, Hikida T. 1988. Karyotypes of Two Species of the Genus Ptychozoon (Gekkonidae: Lacertilia) from Southeast Asia. Japanese journal of herpetology 12(4): 139–141.

Ota H. 1989a. Karyotypes of five species of Gekko (Gekkonidae: Lacertilia) from East and Southeast Asia. Herpetologica 45(4): 438–443.

Ota H. 1989b. A review of the geckos (Lacertilia: Reptilia) of the Ryukyu Archipelago and Taiwan. In: Matsui M, Hikida T, Goris RC. (eds.). Current Herpetology in East Asia. Herpetological Society of Japan, Kyoto. pp. 222–261.

Ota H, Nabhitabhata J. 1991. A new species of Gekko (Gekkonidae: Squamata) from Thailand. Copeia 1991: 503–509.

Ota H, Hikida T, Matsui M, Mori A. 1990. Karyotypes of Gekko monarchus (Squamata: Tokaynidae) from Sarawak, Malaysia. Japanese journal of herpetology 13: 136–138.

Ota H, Hikida T, Nabhitabhata J, Panha S. 2001. Cryptic taxonomic diversity in two broadly distributed lizards of Thailand (Mabuya macularia and Dixonius siamensis) as revealed by chromosomal investigations (Reptilia: Lacertilia).The Natural History Journal of Chulalongkorn University: 1(1), 1–7.

Panitvong N, Sumontha M, Konlek K, Kunya K. 2010. Gekko lauhachindai sp. nov., a new cave-dwelling gecko (Reptilia: Gekkonidae) from central Thailand. Zootaxa 2671: 40–52.

Patawang I, Tanomtong A, Jumrusthanasan S, Kakampuy W, Neeratanaphan L, Pinthong K. 2014. Chromosomal characteristics of NORs and karyological analysis of tokay gecko, Gekko gecko (Gekkonidae, Squamata) from mitotic and meiotic cell division. Cytologia 79(3): 315–324.

Patawang I, Tanomtong A. 2015. The variation of tokay gecko, Gekko gecko (Linnaeus, 1758) between two populations in southern China and Indochinese Peninsula. SWU Science Journal 31(2): 75–186. [in Thai]

Qin XM, Li HM, Zeng ZH, Zeng DL, Guan QX. 2012. Genetic variation and differentiation of Gekko gecko from different populations based on mitochondrial cytochrome b gene sequences and karyotypes. Zoological Science 29: 384–389.

Rösler H, Bauer MA, Heinicke PM, Greenbaum E, Jackman T, Nguyen T, Ziegler T. 2011. Phylogeny, taxonomy, and zoogeography of the genus Gekko Laurenti, 1768 with the revalidation of G. reevesii Gray, 1831 (Sauria: Gekkonidae). Zootaxa 2989: 1–50.

Rujirawan A, Fong JJ, Ampai N, Yodthong S, Termprayoon K, Aowphol A. 2019. A new karst-dwelling gecko of the Gekko petricolus group (Reptilia: Gekkonidae) from Phitsanulok Province, central Thailand. Journal of Natural History 53: 557–576.

Satrawaha R, Ponkanid S. 1988. Chromosome and karyotype of Leiolepis belliana rabritaeniata. KKU Science Journal 17: 152–156.

Smith HM. 1935. Miscellaneous notes on Mexican lizards. The University of Kansas science bulletin 22: 119–155.

Singh L. 1974. Study of mitotic and meiotic chromosomes in seven species of lizards. Proceedings of the Zoological Society 27: 57–79.

Solleder E, Schmid M. 1984. XX/XY sex chromosomes in Gekko gecko (Sauria, Reptilia). Amphibia-Reptilia 5: 339–345.

Sharma OP, Tripathi NK, Sharma KK. 2002. A review of chromosome banding in fishes. In: Sobti RC, Obe G, Athwal RS. (eds.). Some Aspects of Chromosome Structure and Functions. New Narosa Publishing House, New Delhi. pp. 109–122.

Srikulnath K, Uno Y, Nishida C, Ota H, Matsuda Y. 2015. Karyotype reorganization in the Hokou Gecko (Gekko hokouensis, Gekkonidae): The Process of Microchromosome Disappearance in Gekkota. PLoS ONE 10(8). https://doi.org/10.1371/journal.pone.0134829

Shibaike Y, Takahashi Y, Arikura I, Iiizumi R, Kitakawa S, Sakai M, Imaoka C, Shiro H, Tanaka H, Akakubo N, Nakano M, Watanabe M, Ohne K, Kubota S, Kohno S, Ota H. 2009. Chromosome evolution in the lizard genus Gekko (Gekkonidae, Squamata, Reptilia) in the East Asian islands. Cytogenetics and Genome Research 127: 182–190.

Solleder E, Schmid M. 1984. XX/XY sex chromosomes in Gekko gecko (Sauria, Reptilia). Amphibia-Reptilia 5: 339–345.

Toda M. 2008. External characters useful to discriminate Gekko hokouensis and its cryptic undescribed species of the Okinawa Group, Japan: A preliminary report. Akamata 19: 23–30.

Trifonov VA, Giovannotti M, O’Brien PCM, Wallduck M, Lovell F, Rens W, Parise-Maltempi, PP, Caputo V, Ferguson-Smith MA. 2011. Chromosomal evolution in Gekkonidae. I. Chromosome painting between Gekko and Hemidactylus species reveals phylogenetic relationships within the group. Chromosome Research 19: 843–855.

Turpin R, Lejeune J. 1965. Les Chromosomes Humains. Paris: Gauther Villars. 535 pp.

Volobouev V, Pasteur G, Ineich I, Dutrillaux B. 1993. Chromosomal evidence for a hybrid origin of diploid parthenogenetic females from the unisexual-bisexual Lepidodactylus lugubris complex (Reptilia, Gekkonidae). Cytogenetics Cell Genetics 63: 194–199.

Wongwattana V, Pariyanonth P, Chulalaksananukul W. 2001. Karyological studies of lizard, Leiolepis belliana belliana at Sa-Mae Sarn Island and nearby Islands in Chon Buri Province. M.Sc. thesis, Chulalongkorn University, Bangkok.

Wu G, Zhao E. 1984. Studies on karyotypes of Gekko gecko and Gekko subpalmatus. Acta Herpetologica Sinica 3: 61–64.

Yoshida M, Itoh M. 1974. Karyotype of the gecko, Gekko japonicus. Chromosome Information Service 17: 29–31.

Yüksel E, Gaffaroğlu M. 2008. The analysis of nucleolar organizer regions in Chalcalburnus mossulensis (Pisces: Cyprinidae). Journal of Fisheriessciences.com 2: 587–591.

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Published

2022-09-21

How to Cite

Thongnetr, W., Aiumsumang, S. ., Tanomtong, A., & Phimphan, S. (2022). Classical chromosome features and microsatellites repeat in Gekko petricolus (Reptilia, Gekkonidae) from Thailand. Caryologia, 75(2), 81-88. https://doi.org/10.36253/caryologia-1544

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