Cytogenetic study of the Bent-toed Gecko (Reptilia, Gekkonidae) in Thailand; I: Chromosomal classical features and NORs characterization of Cyrtodactylus kunyai and C. interdigitalis

  • Weera Thongnetr
  • Alongklod Tanomtong
  • Suphat Prasopsin
  • Nuntiya Maneechot
  • Krit Pinthong
  • Isara Patawang
Keywords: Cyrtodactylus, Cyrtodactylus kunyai, Cyrtodactylus interdigitalis, Karyotype stasis

Abstract

This study analysed the karyotype of Cyrtodactylus kunyai and C. interdigitalis from Loei Province in Northeastern Thailand. The metaphase and meiotic chromosome preparations were obtained by squash technique from bone marrow and testes, respectively. The chromosomes were stained by Giemsa staining and Ag-NOR-banding techniques. The results showed diploid chromosome number (2n) of 40 for C. kunyai and 42 for C. interdigitalis. The chromosome types of metacentric, submetacentric, acrocentric and telocentric chromosomes were 8-4-0-28 and 4-2-4-32, respectively. The Ag-NORs banding technique provides the pair of nucleolar organizer regions (NORs) of both two species at telomeric region of the long arm of the pair 12, metacentric type in C. kunyai and telocentric type in C. interdigitalis. There are no sex differences in karyotypes between males and females of both two species. We found that during metaphase I on meiosis of C. kunyai and C. interdigitalis, the homologous chromosomes showed synapsis of 20 and 21 bivalents, respectively. Moreover, the meiotic phase on prophase II exhibited 20 and 21 haploid chromosome number (n) as respective diploid species. Their karyotype formulas is as follows: C. kunyai (2n = 40): Lm2 + Lsm4 + Lt4 + Mt6 + Sm6 + St18, and C. interdigitalis (2n = 42): La4 + Lt14 + Mt2 + Sm4 + Ssm2 + St16.

Author Biographies

Weera Thongnetr

Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, Thailand

Walai Rukhavej Botanical Research Institute, Mahasarakham University, Kantharawichai, Maha Sarakham, Thailand

Alongklod Tanomtong

Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, Thailand

Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University, Muang, Khon Kaen, Thailand

Suphat Prasopsin

Research Academic Supports Division, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi, Thailand

Nuntiya Maneechot

Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Muang, Surin, Thailand

Krit Pinthong

Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Muang, Surin, Thailand

Isara Patawang

Department of Biology, Faculty of Science, Chiang Mai University, Muang, Chiang Mai, Thailand

Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Muang, Chiang Mai, Thailand

Published
2019-05-10
How to Cite
Thongnetr, W., Tanomtong, A., Prasopsin, S., Maneechot, N., Pinthong, K., & Patawang, I. (2019). Cytogenetic study of the Bent-toed Gecko (Reptilia, Gekkonidae) in Thailand; I: Chromosomal classical features and NORs characterization of Cyrtodactylus kunyai and C. interdigitalis. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 72(1), 23-28. https://doi.org/10.13128/cayologia-248
Section
Articles