First report of karyological analysis and heteromorphic nucleolar organizer region of Black Surgeonfish (Acanthurus gahhm, Acanthuridae) in Thailand


  • Sitthisak Jantarat Program of Biology, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Thailand
  • Sarun Jumrusthanasan Biology Program, Faculty of Science, Buriram Rajabhat University, Buriram, Thailand
  • Sarawut Kaewsri Biology Program, Faculty of Science, Buriram Rajabhat University, Buriram, Thailand
  • Praween Supanuam Biology Program, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani, Thailand
  • Alongklod Tanomtong Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand



Acanthurus gahhm, Chromosome, Karyotype, NORs


This research was the first report on karyological analysis and heteromorphic nucleolar organizer region of black surgeonfish (Acanthurus gahhm, Acanthuridae) in Thailand. The 10 male and 10 female specimens were collected from Phuket Marine Biological Center, and Phang Nga Coastal Research and Development Center, Andaman Sea, Thailand. Mitotic chromosomes were directly prepared from gill and kidney tissues. The chromosomes were stained by conventional Giemsa staining and Ag-NOR banding techniques. Results showed that the diploid chromosomes number of A. gahhm was 2n=48, the fundamental numbers (NF) was 54 in both male and female. The karyotype consist of 6 large acrocentric, 20 large telocentric, 18 medium telocentric and 4 small telocentric chromosomes. None of strange size chromosomes related to sex was found. The heteromorphic nucleolar organizer regions (NORs) were observed on telomeric short arm of first acrocentric which can defined as 1a1b. There is NOR in 1a and not in 1b. The karyotype formula of black surgeon fish was as follows: 2n (48) = La6+Lt20+Mt18+St4


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Affonso, PRAM, Fernandas MA, Almeida JS, Molina WF. 2014. Sequential steps of chromosomal differentiation in Atlantic surgeonfishes: evolutionary inferences. The Scientific World Journal. 2014: 825703. doi: 10.1155/2014/825703.
Allen G, Steene R, Humann P, Deloach N. 2012. Reef Fish Identification: Tropical Pacific. 5th ed. Florida: New World Publications.
Arai R. 2011. Fish karyotypes a check list. Japan: Springer.
Arai R, Inoue M. 1976. Chromosomes of seven species of Pomacentridae and two species of Acanthuridae from Japan. Bull. Natn. Sci. Mus. Tokyo, (A). 2: 73–78.
Carpenter KE, Niem VH. 2001. The Living marine resources of the Western Central Pacific. Rome: Food and Agriculture Organization of the United Nations.
Chaiyasut K. 1989. Cytogenetics and cytotaxonomy of the genus Zephyranthes. Bangkok: Department of Botany, Faculty of Science, Chulalongkorn University. Thailand.
Galetti JPM, Molina WF, Affonso, PRAM, Aguilar CT. 2006. Assessing genetic diversity of Brazilian reef fishes by chromosomal and DNA markers. Genetica. 126:161–177.
Howell WM, Black DA. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 36:1014–1015.
Le Grande, WH, Fitzsimons JM. 1988. Chromosome numbers of some gulf coast sciaenid fishes. Copeia. 2: 491–493.
Monkolprasit S, Sontirat S, Vimollohakarn S, Songririkul T. 1997. Checklist of fishes in Thailand. Bangkok: Office of Environmental Policy and Planning.
Ohno S. 1970. Evolution by gene duplication. New York: Springer-Verlag.
Ojima Y, Yamamoto K. 1990. Cellular DNA contents of fishes determined by flow cytometry. La Kromosomo, II. 57:1871–1888.
Phuket Marine Biological Center. 2007. A guide to reef fishes of the Andaman Sea, Thailand. Phuket: Phuket Marine Biological Center, Dpartment of Marine and Coastal Resources. Thai.
Tanomtong A. 2019. Cyogenetics. Bangkok; Chulalongkorn University Press. Thailand.
Turpin R, Lejeune J. 1965. Les Chromosomes Humains. Paris: Gautier-Villars. French.
Verma RS, Babu A. 1995. Human chromosomes principles and techniques. 2nd ed. New York: McGraw-Hill Inc.




How to Cite

Jantarat, S., Jumrusthanasan, S., Kaewsri, S., Supanuam, P., & Tanomtong, A. (2021). First report of karyological analysis and heteromorphic nucleolar organizer region of Black Surgeonfish (Acanthurus gahhm, Acanthuridae) in Thailand. Caryologia, 74(1), 83–88.




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