Clethodim induced pollen sterility and meiotic abnormalities in vegetable crop Pisum sativum L.

  • Sazada Siddiqui Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
  • Sulaiman Al-Rumman Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
Keywords: Herbicide, Clethodim, Pollen sterility, Meiotic abnormality, Pisum sativum L.

Abstract

Pesticides are highly noxious materials.Their poisonousness might not be unequivocally precise to the target entities but can unfavorably disturb various procedures in the non-target host plants. In the present study, the effect of application of clethodim on pollen sterility and meiotic anomalies of Pisum sativum L are studied. Pisum sativum L seeds are treated with different concentrations of clethodim varying from 0.01%, 0.02%, 0.03%, and 0.04% for exposure time of 1 hour and their effect on pollen sterility and chromosomal anomalies were investigated. The outcomes reveal that treatment of clethodim on Pisum sativum L seeds induces pollen sterility (PS) and chromosomal anomalies (CA) in a dose-dependent manner. Also in clethodim treated seeds, an elevation in the proportion of abnormal meiotic phases were observed which was time and concentration dependent. Secondary association (SeA), precociuos separation (PS), clumped nuclei (CNu) were reported in metaphase I & II, stickiness (Stc), bridges (Br) and laggards (Lg) in anaphase I & II. The results of the present study reveal that frequently used herbicide clethodim has a substantial cytotoxic effect on meiotic cells of Pisum sativum L.

References

Abdel-Rahmam AR, Wauchope RD, Truman CC, Dowler CC. 1999. Runoff and leaching of atrazine and alachlor on a sandy soil as affected by application in sprinkler irrigation. Journal of Environmental Science and Health-B: Pesti and Food Cont. 34:381-396.

Anila MD, Ditika K. 2013. Cytotoxic and genotoxic potency screening of two pesticides on Allium cepa L. Proc Tech. 8:9–26.

Arzu O, Dilek A, Yasin E, Sevim FE. 2014. Potential cytotoxic effect of anilofos by using Allium cepa assay. Cytotechnology 67:783–791.

Çalli İÖ. 2008. Effects of fungicide on meiosis of tomato (Lycopericon esculentum Mill.). Bangladesh J Bot. 37(2):121-125.

Chevreuil M, Garmouma M, Teil MJ, Chesterikoff A.1996. Occurrence of organochlorines (PCBs, pesticides) and herbicides (triazines, phenylureas) in the atmosphere and in the follout from urban and rural stations of Paris area. Science of the Environ. 182:25-37.

Das A, Dutta S, Nag S, Mandal S. 2018. Effect of acephate pesticide on meiosis in onion (Allium cepa). Inter J of Agri. 10(6):5456-5459.

Davies DR, Berry GJ, Heath MC, Dawkins TCK .1985. Pea (Pisum sativum L.). In: Summerfield RJ, Roberts EH (Eds.). Grain legume crops. Williams Collins (London). p. 147–198.

Deveci A, Aksoy O, Al G. 2017. Investigation of the effects of quizalofop-P-ethyl on pollen germination of Hyacinthus orientalis L. Caryologia. 70:77–81.

Dewitte A, Eeckhaut T, Van Huylenbroeck J, Van Bockstaele E. 2010. Meiotic aberrations during 2n pollen formation in Begonia. Heredity. 104:215–23.

Dubey PS, Shrivastava A, Shevade A. 1977. Pesticidal toxicity bioassay with pollen damage. Environ. Poll. 13(3):169-171.
Edwards D. 2005. Reregistration Eligibility Decision (RED) for Sethoxydim. United States Environmental Protection Agency. p. 1–27.

Gomurgen AN. 2000. Cytological effect of the herbicide 2,4-D isooctylester 48% on root mitosis of Allium cepa. Cytologia. 65:383–388.

Gorell JM, Jhonson CC, Rybicki, BA, Peterson EL, Ricchardson RJ. 1998. The Risk of Parkinson’s disease with exposure to pesticides, farming, well water, and rural living. Neurology Heidelberg. 50:1346-1350.

He H, YuJ, Chen G, Li W, He J, Li H. 2012. Acute toxicity of butachlor and atrazine to reshwater green alga Scenedesmus obliquus and cladoceran Daphnia carinata. Ecotox Environ Saf. 80:91-96.

He Y, Wetzstein HY, Palevitz BA. 1995. The effects of a triazole fungicide, propiconazole, on pollen germination, tube growth and cytoskeletal distribution in Tradescantia virginiana. Sex Plant Reprod. 8:210-216.
Evans HJ. 1962. Chromosome aberrations induced by ionizing radiations. Int Rev Cytol. 13:221–232.

Grant WF. 1978. Chromosome aberrations in plant as a monitoring system. Environ Health Per. 27:37–43.

Kaufman BP, McDonald MR, Bernstein MH. 1955. Cytochemical studies of changes induced in cellular materials by ionizing radiations. Ann N Y Acad Sci. 59:553-566.

Tartar G, Kaymak F, Muranli FDG. 2006. Genotoxic effects of Avenoxan on Allium cepa L. and Allium sativum L. Caryologia. 59:241–247.

Jayabalan N, Rao GR. 1987. Gamma radiation induced cytological abnormalities in Lycopersicon esculentum Mill Var, Pusa Ruby. Cytologia. 52:1–4.

Hirayoshi I. 1957. The chromosomal relationships in Oryzeae and Zizanieae. Cytologia Supp. Vol Proc Int Genet Symposia. 1956:293-297.

Stebbins GL. 1950. Variation and Evolution in Plants. Columbia Univ Press. New York.

Bhattacharya A, Datta AK. 2010. Secondary chromosome associations in Uraria picta (Jacq.) DC. (Family: Leguminosae). Cytologia. 75:37–40.

Girjesh K, Nitu C. 2014. Secondary chromosomal association in kidney bean (Phaseolus vulgaris L.). Jordan J Biological Sci. 7(1):71-74.

Siddiqui S. 2015. DNA damage in Cicer plant grown on soil polluted with heavy metals. J of King Saud University – Sci. 27:217–223.

Umar G, Singh V. 2003. Comparative analysis of meiotic abnormalities induced by gamma rays and EMS in Barley. J Indian Bot Soc. 82:19–22.

Rank J, Jensen AG, Skov B, Pedersen LH, Jensen K. 1993. Genotoxicity testing of the herbicide Roundup and its active ingredient glyphosate isopropylamine using the mouse bone marrow micronucleus test, Salmonella mutagenicity test and Allium anaphase–telophase test. Mutat Res/Gene Toxic. 300:29–36.

Gichner T, Menke M, Stavreva DA, Schubert I. 2002. Maleic hydrazide induces genotoxic effects but no DNA damages detectable by the comet assay in tobacco and field bean. Mutagenesis. 15:385–389.

Barthelmess A. 1957. Chemish induzierte multipalare Mitosen. Protoplasma. 48:546–561.
Coldecott RS. Smith L. 1952. A study of X ray induced chromosamal aberrations in barley. Cytologia. 17:224–242.

Rao NB, Laxmi N. 1980. Gamma rays induced meiotic abnormalities in Capsicum annum L. Caryologia. 33:509-518.

Mukherjee M, Datta AK. 2005. Secondary chromosome associations in Ocimum basilicum L. and Ocimum tenuiflorum L. Cytologia.70:149–152.

El-Ghamery AA, El-Nahas AI, Mansour MM. 2000. The action of atrazine herbicide as an inhibitor of cell division on chromosomes and nucleic acids content in root meristems of Allium cepa and Vicia faba. Cytologia. 65:277–287.

Kaur P, Grover IS. 1985. Cytological effects of some organophosphorus pesticides. II. Meiotic effects. Cytologia. 50:199–211.

Kim JH, Feagley SE. 1998. Adsorption and leaching of trifluralin, metolachlor, and metribuzin in a commerce soil. Journal of Environmental Science and Health-B: Pest and Food Conta. New York. 33:529-546.

Liang GH, Feltner KC, Liang YYS, Morril JM. 1967. Cytogenetic effects and res ponses of agronomic characters on grain sorghum following atrazine application. Crop Sci. 7:245-248.
Manton I. 1950. Problems of cytology and evolution in the Pteridophyta. Cambridge: Cambridge University Press; p. 316.

Munger R, Isacson P, Hu S, Burns T, Hanson J, Lynch CF, Cherryholmes K, Vandorpe P, Hausler JrWJ. 1997. Intrauterine growth retardation in Iowa communities with herbicides- contaminated drinking water supplies. Environ Health Persp. 105:308-314.

Namrata S, Alka S. 2014. Biomonitoring of Genotoxic Effect of Glyphosate and Pendimethalin in Vigna mungo Populations. Cytologia. 79(2):173-180.

Novak F. Betlach J. 1970. Meiotic irregularities in pollen sterile sweet pepper. Cytologia. 35:335-343.
Nwani CD, Nagpure NS, Kumar R, Kushwaha B, Kumar P, Lakra, WS. 2011. Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater fish Channa puntatus (Bloch) using micronucleus test and single cell gel electrophoresis. Envir Toxic and Pharm. 31:314-322.

Ramanna MS. 1974. The origin of unreduced microspores due to aberrant cytokinesis in meiocytes of potato and its genetic significance. Euphytica. 23:20-30.
Rana SR, Swaminathan MS. 1964. Cytological aspects of pollen sterility. Recent Advances in Palynology. p. 276-304. National Botanical garden, Lucknow

Siddiqui S, Khan SS, Meghvansi MK, Nazoora SA. 2008. Mutagenic effect of herbicide maleic hydrazide on seed germination and radicle length on Trigonella foenum-graecum. Indian J Appl Pure Biol. 23:103-106.

Siddiqui S, Meghvansi MK, Khan SS. 2012. Glyphosate, alachor and maleic hydrazide have genotoxic effect on Trigonella foenum-graecum L. Bull Environ Contam Toxicol. 88:659–665.

Siddiqui S, Meghvansi MK, Wani MA, Jabee F. 2009. Evaluating cadmium toxicity to the root meristem of Pisum sativum L. Acta Physiol Plant. 31:531–536.

Sinha SSN, Godward MBE. 1972. Radiation studies in Lens culinaris. Meiosis: abnormalities induced due to gamma radiation and its consequences. Cytologia. 37:685-695.

Sinha, SSN, Godward MBE. 1969. Radiation studies in Lens culinaris. Mitosis type of aberration and their elimination in root and shoot apical meristem. J Cytol Genet. 3:80-91.

Soheir E, Antoinette H, Atif H. 1989. Cytological effect of herbicide Garlon-4 on root mitosis of Allium cepa. Cytologia. 54:465-472.

Timbrell JA. Introduction to Toxicology. 2. Ed. Estados Unidos: Taylor & Francis, 1999. p.167
Umar G, V Singh. 2003. Comparative analysis of meiotic abnormalities induced by gamma rays and EMS in Barley. J Indian Bot Soc. 82:19–22.

Vander Werf HMG. 1996. Assessing the impact of pesticides on the environment. Agriculture, Ecosystems and Environment, The Netherlands. 60:81-96.

Zhang W, Jiang F, Ou J. 2011. Global pesticide consumption and pollution: with Chinas as a focus. Proceedings of the Inter Acad of Ecol and Envir Scies. 1(2):125-144.
Published
2020-03-13
How to Cite
Siddiqui, S., & Al-Rumman, S. (2020). Clethodim induced pollen sterility and meiotic abnormalities in vegetable crop Pisum sativum L. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 73(1). https://doi.org/10.13128/caryologia-269
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Articles