Clethodim induced pollen sterility and meiotic abnormalities in vegetable crop Pisum sativum L.
Keywords:Herbicide, Clethodim, Pollen sterility, Meiotic abnormality, Pisum sativum L.
Pesticides are highly noxious materials.Their poisonousness might not be unequivocally precise to the target entities but can unfavorably disturb various procedures in the non-target host plants. In the present study, the effect of application of clethodim on pollen sterility and meiotic anomalies of Pisum sativum L are studied. Pisum sativum L seeds are treated with different concentrations of clethodim varying from 0.01%, 0.02%, 0.03%, and 0.04% for exposure time of 1 hour and their effect on pollen sterility and chromosomal anomalies were investigated. The outcomes reveal that treatment of clethodim on Pisum sativum L seeds induces pollen sterility (PS) and chromosomal anomalies (CA) in a dose-dependent manner. Also in clethodim treated seeds, an elevation in the proportion of abnormal meiotic phases were observed which was time and concentration dependent. Secondary association (SeA), precociuos separation (PS), clumped nuclei (CNu) were reported in metaphase I & II, stickiness (Stc), bridges (Br) and laggards (Lg) in anaphase I & II. The results of the present study reveal that frequently used herbicide clethodim has a substantial cytotoxic effect on meiotic cells of Pisum sativum L.
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