Impact of Bisphenol A on seed germination, radicle length and cytogenetic alterations in Pisum sativum L.

Authors

  • Sazada Siddiqui Department of Biology, College of Science, King Khalid University, Abha
  • Saad Abdurahamn Muhammad Al Amri Department of Biology, College of Science, King Khalid University, Abha
  • Huda Ahmed Al Ghamdy Department of Biology, College of Science, King Khalid University, Abha
  • Wadha Saad Saeed Alqahtani Department of Biology, College of Science, King Khalid University, Abha
  • Sarah Mohammed Alquyr Department of Biology, College of Science, King Khalid University, Abha
  • Habab Merghani Yassin Department of Biology, College of Science, King Khalid University, Abha

DOI:

https://doi.org/10.36253/caryologia-1230

Keywords:

BPA, Seed germination, Mitotic index, Chromosomal anomalies, Pisum sativum L.

Abstract

Bisphenol A (BPA) is a global transpiring pollutant and an endocrine disruptor present in the environment which has a substantial harmful effect on plants. In the present study, its effects on seed germination, radicle length and cytogenetic alterations were investigated in P. sativum root tip cells. P. sativum seeds were germinated after treating with various concentrations of BPA (2 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, 20 mg/L and 25 mg/L) at 24±1°C for 72 hours and the cytogenetic variations were assessed. The investigation showed that BPA reduced the percentage of seed germination, mitotic index, radicle length (at higher concentrations) and instigated a rise in chromosomal anomalies in a dose-related manner. In total, there is an enhanced occurrence of c-mitosis, stickiness, bridges, fragments and laggards in the BPA treated root tip cells of P. sativum seeds.

Downloads

Download data is not yet available.

References

Adamakis IDS, Emmanuel P, Anna C, Eleftherios PE. 2013. Effects of bisphenol A on the microtubule arrays in root meristematic cells of Pisum sativum L. Mutation Research.750:111–120.

Adamakis IDS, Emmanuel P, Eleftherios PE. 2016. Bisphenol A disrupts microtubules and induces multipolar spindles in dividing root tip cells of the gymnosperm Abies cephalonica. Chemosphere.149:202–210.

Adamakis IDS, Panteris E, Eleftheriou EP. 2019. Tubulin acetylation mediates bisphenol a effects on the microtubule arrays of Allium cepa and Triticum turgidum. Biomolecules. 9(5):185.

Amer Al-Hiyasat. 2017. The effect of bisphenol A on root development and chlorophyll a:b ratio in lens culinaris. International J. of Sciences: Basic and Appl Res. 34(3):115–123.

Can A, Semiz O, Cinar O. 2005. Bisphenol A induces cell cycle delay and alters centrosome and spindle microtubular organization in oocytes during meiosis. Mol Hum Reprod.11:389–396.

Clarke BO, Smith SR. 2011. Review of “emerging” organic contaminants in biosolids and assessment of international research priorities for the agricultural use of biosolids. Environ Int. 37:226–247.

Cooper JE, Kendig EL, Belcher SM. 2011. Assessment of bisphenol A released from reusable plastic, aluminium and stainless-steel water bottles. Chemosphere. 85:943–947.

Dokyung Kim, Jin Il Kwak, Youn-Joo An. 2018. Effects of bisphenol A in soil on growth, photosynthesis activity, and genistein levels in crop plants (Vigna radiata). Chemosphere. 209: 875-882.

Eunkyoo O, Jonghyun K, Eunae P, Jeong K, ChangwonK, Giltsu C. 2004. PIL5, a phytochrome-interacting basic helix-loop-helix protein, is a key negative regulator of seed germination in Arabidopsis thaliana. Plant Cell. 16: 3045–3058.

Ferrara G, Loffredo E, Senesi N. 2006. Phytotoxic, clastogenic and bioaccumulation effects of the environmental endocrine disruptor bisphenol A in various crops grown hydroponically. Planta 223:910–916.

Fu P, Kawamura K. 2010. Ubiquity of bisphenol A in the atmosphere. Environ Pollut.158: 3138–3143.

Gatidou G, Thomaidis NS, Stasinakis AS, Lekkas TS. 2007. Simultaneous determination of the endocrine disrupting compounds nonylphenol, nonylphenol ethoxylates, triclosan and bisphenol A in wastewater and sewage sludge by gas chromatography-mass spectrometry. J. Chromatography A. 1138:32–41.

George O, Bryant BK, Chinnasamy R, Corona C, Arterburn JB, Shuster CB. 2008. Bisphenol A directly targets tubulin to disrupt spindle organization in embryonic and somatic cells. ACS Chem Biol 2008 3(3):167–79.

Gomurgen AN. 2000. Cytological effect of the herbicide 2,4-D isooctylester 48% on root mitosis of Allium cepa. Cytologia. 65: 383–388.

Hanumappa M, Pratt LH, Cordonnier-Pratt MM, Deitzer GF. 1999. A photoperiod-insensitive barley line contains a light-labile phytochrome B1. Plant Physiol. 119:1033–1039.

Huang H, Tan WJ, Wang CC, Leunga LK. 2012. Bisphenol A induces corticotropin-releasing hormone expression in the placental cells JEG- 3. Reprod Toxicol. 34: 317–322.

Isabelle D, Leon-Kloosterziel KM, Koomneel M. 2000. Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis. Plant Physiology. 122:403–413.

Jadhav VV, Jadhav AS, Chandagade CA, Raut PD. 2012. Genotoxicity of bisphenol a on root meristem cells of Allium cepa: A Cytogenetic Approach. Asian J. of Water, Envir and Poll. 9:(1) 39–43.

Le HH, Carlson EM, Chua JP, Belcher SM. 2008. Bisphenol A is released frompolycarbonate drinking bottles and mimics the neurotoxic action of estrogen in developing cerebellar neurons. Toxic Lett. 176: 149–156.

Li MY, Qin CB, Ruth W, Wang XM.2006. Double knockouts of phospholipases Df1 and Df2 in Arabidopsis affect root elongation during phosphate-limited growth but do not affect root hair patterning. Plant Physiol.140:761–770.

Mello MLS, Vidal BC. 1978. A reação de Feulgen. Ciênc Cult. 30:665–676.

Mihaich EM, Friederich U, Caspers N, Hall T, Klecka GM, Dimond SS, Staples CA, Ortego LS, Hentges SG. 2009. Acute and chronic toxicity testing of bisphenol A with aquatic invertebrates and plants. Ecotox Environ Safe. 72:1392–1399.

Mita L, Bianco M, Viggiano E, Zollo F, Bencivenga U, Sica V, Monaco G, Portaccio M, Diano N, Colonna A, Lepore M, Canciglia P, Mita DG. 2011. Bisphenol A content in fish caught in two different sites of the Tyrrhenian Sea (Italy). Chemosphere. 82: 405–10.

Nasir J, Austin RS, Janak L, Pathaka, Shi Y, Chang YC. 2018. Bisphenol A (BPA) the mighty and the mutagenic. Toxicology Reports. 5: 76–84.

Noureddin M, Furumoto T, Ishida Y, Fukui H. 2004. Absorption and metabolism of bisphenol A, a possible endocrine disruptor, in the aquatic edible plant, water convolvulus (Ipomoea aquatica), Biosci Biotechn Bioch. 68:1398–1402.

Ohashi Y, Oka A, Rodrigues-Pousada R, Possenti M, Ruberti I, Morelli G, Aoyama T. 2003. Modulation of phospholipid signaling by GLABRA2 in roothair pattern formation. Science. 300:1427–1430.

Ozge K, Walid R, David B, Pınar E, Hussein FK, Belma KG. 2019. The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE-1 cells: A comparative study. J Appl Toxicol. 1–12.

Palani KL, Panneerselvam N. 2007. Cytogenetic studies of food preservative in Allium cepa root meristem cells. Facta universitatis Series: Medi. and Bio. 14(2): 60–63.

Pan WJ, Can X, Qiu-P W, Jin-XL, Hong-ML, Wei C, Yong-SL, Lei Z. 2013. Effect of BPA on the germination, root development, seedling growth and leaf differentiation under different light conditions in Arabidopsis thaliana. Chemosphere. 93: 2585–2592.

Pothitou P, Voutsa D. 2008. Endocrine disrupting compounds in municipal and industrial wastewater treatment plants in Northern Greece, Chemosphere. 73:1716–1723.

Qiu Z, Wang L, Zhou Q. 2013. Effects of bisphenol A on growth, photosynthesis and chlorophyll fluorescence in above-ground organs of soybean seedlings. Chemosphere. 90:1274–280.

Rank J. 2003. The method of Allium anaphase-telophase chromossome aberration assay. Ekologija .1:38–42.

Saini S, Sharma I, Kaur N, Pati PK. 2013. Auxin: a master regulator in plant root development. Plant Cell Reports. 32: 741–757.

Shahin SA, El-Amoodi KHH. 1991. Induction of numerical chromosomal aberrations DNA synthesis using the fungicides nimrod and rubigan-4 Vicia faba L. Mutat. Res. 261: 169–176.

Siddiqui S, Al-Rumman S. 2020a. Cytological changes induced by clethodim in Pisum sativum plant. Bangladesh J. Bot. 49(2): 367–374.

Siddiqui S, Al-Rumman S. 2020b. Clethodim induced pollen sterility and meiotic abnormalities in vegetable crop Pisum sativum L. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics 73(1): 37–44.

Siddiqui S, Meghvansi MK, Hasan Z. 2007. Cytogenetic changes induced by sodium azide (NaN3) on Trigonella foenum-graecum L. seeds. South African J of Bot. 73: 632–635.

Speranza A, Crosti P, Malerba M, Stocchi O, Scoccianti V. 2011. The environmental endocrine disruptor, bisphenol A, affects germination, elicits stress response and alters steroid hormone production in kiwifruit pollen. Plant Biol. 13: 209–217.

Staples C, Friederich U, Hall T, Kleˇcka G, Mihaich E, Ortego L, Caspers N, Hentges S. 2010. Estimating potential risks to terrestrial invertebrates and plants exposed to bisphenol A in soil amended with activated sludge biosolids. Environ Toxicol Chem. 29: 467–475.

Staples CA, Dorn PB, Kle_GM, O'Block ST, Harris LR. 1998. A review of the environmental fate, effects and exposures of bisphenol A. Chemosphere. 36: 2149–2173.

Stasinakis AS, Gatidou G, Mamais D, Thomaidis NS, Lekkas TS. 2008. Occurrence and fate of endocrine disrupters in Greek sewage treatment plants, Water Res. 42: (2008) 1796–1804.

Wagner M, Oehlmann J. 2009. Endocrine disruptors in bottled mineral water: total estrogenic burden and migration from plastic bottles. Environ Sci Pollut Res. 16: 278–286.

Xin F, L Jiang, Liu X, Geng C, Wang W, Zhong L, Yang G, Chen M. 2014. Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells. Mutat Res Genet Toxicol Environ Mutagen. 769: 29–33.

Yamamoto T, Yasuhara A, Shiraishi H, Nakasugi O. 2001. Bisphenol A in hazardous waste landfill leachates. Chemosphere. 42: 415–418.

Zhiyong Q, Lihong W, Qing Z. 2013. Effects of bisphenol A on growth, photosynthesis and chlorophyll fluorescence in above-ground organs of soybean seedlings.Chemosphere. 90(3):1274–80.

Downloads

Published

2021-10-08

How to Cite

Siddiqui, S., Abdurahamn Muhammad Al Amri, S. ., Ahmed Al Ghamdy, H. ., Saad Saeed Alqahtani, W. ., Mohammed Alquyr, S. ., & Merghani Yassin, H. . (2021). Impact of Bisphenol A on seed germination, radicle length and cytogenetic alterations in Pisum sativum L. Caryologia, 74(2), 103-109. https://doi.org/10.36253/caryologia-1230

Issue

Section

Articles