Anther structure and pollen development in Jurinea kilaea Azn. (Asteraceae)

Authors

DOI:

https://doi.org/10.36253/caryologia-2965

Keywords:

anther wall, microsporogenesis, pollen development, Jurinea kilaea, Asteraceae

Abstract

In this study, anther wall structure and the embryological features of male gametophyte development in Jurinea kilaea from Asteraceae family are described for the first time. Capitula of different sizes containing young flower buds of J. kilaea was collected from Tekirdağ, Saray - Kastro coast in July 2022 – 2024. Anthers separated according to their sizes under a stereo microscope were passed through arising alcohol series and embedded in Hisstore. Toluidine blue O solution was used to stain the sections. Slides were examined with light microscope and photographed by an Olympus E330 camera. In J. kilaea, anthers are tetrasporangiate. Anther wall consists of the outermost epidermis, the endothecium, the middle layer and the innermost tapetum layer. Tapetum cells appear to have 1 or 2 nuclei. Tapetum is plasmodial type and, tapetum cells begin to degenerate towards the end of the tetrad phase. Microsporogenesis and pollen mitosis are generally regular. Asynchrony is observed during meiosis in young anther loci. Generally, decussate type tetrad was observed. Rarely pentads were also observed. Cytoplasmic channels were observed between microspores at different stages of microsporogenesis. The mature pollen grains of J. kilaea are generally composed of three nuclei and have a normal structure. However, there have been instances where pollen grains exhibit an abnormal structure. Pollen sterility ratio was found to be 12.1%.

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References

Ağır SU, Kutbay HG, Sürmen B. 2016. Plant diversity along coastal dunes of the Black Sea (North of Turkey). Rend Lincei 27: 443-453.

Ağır SU, Kutbay HG, Sürmen B, Elmas E. 2017. The effects of erosion and accretion on plant communities in coastal dunes in north of Turkey. Rend Lincei. 28: 203-224.

Ağır SU, Sürmen B, Kutbay HG, İmamoğlu A. 2021. Species importance in coastal dune ecosystems in Northern Turkey. Ann Bot. 13-32.

Ao C, Wang L, Liang L, Wang X. 2009. Anther wall formation, microsporogenesis and male gametogenesis of four closely related species in Astereae (Asteraceae): description, comparison and systematic implications. Nord J Bot. 27(4): 292-297.

Avcı M, Avcı S, Akkurt S. 2015 (October). Coastal dune vegetation in Turkey: a geographical perspective. In International Conference on the Mediterranean Coastal Environment MEDCOAST pp. 6-10.

Baquar SR, Husain SA. 1969. Cytoplasmic channels and chromatin migration in the meiocytes of Arnebia hispidissima (Sieb.) DC. Ann Bot. 33(4): 821-831.

Bona M. 2020. Systematic importance of achene macro‐micromorphological characteristics in selected species of the genera Crupina, Jurinea, and Klasea (Asteraceae) from Turkey. Microsc Res Tech. 83(11): 1345-1353.

Çetinbaş A, Ünal M. 2015. Anther ontogeny and microsporogenesis in Helianthus annuus L. (Compositae). Not Sci Biol. 7(1): 52-56.

Chehregani A, Salehi H. 2016. Male and female gametophyte development in Achillea tenuifolia (Asteraceae). P Bio Sci. 6(1): 85-94.

Davis PH. 1975. Flora of Turkey and the East Aegean Islands. Vol.5, Edinburg University Press, Edinburg, Great Britain, pp. 1-43: 370-381.

Doğan B, Hakkı EE, Duran A. 2010. A phylogenetic analysis of Jurinea (Compositae) species from Turkey based on ITS sequence data. Afr J Biotechnol. 9(12): 1741-1745.

Gabarayeva N, Polevova S, Grigorjeva V, Severova E, Volkova O, Blackmore S. 2019. Suggested mechanisms underlying pollen wall development in Ambrosia trifida (Asteraceae: Heliantheae). Protoplasma 256: 555-574.

Gotelli M, Lattar E, Zini LM, Rosenfeldt S, Galati B. 2023. Review on tapetal ultrastructure in angiosperms. Planta 257(6): 100.

Gülez S, Kaya LG, Dönmez S, Çetinkale SG, Koçan N. 2007. A study on the landscape design for the mugada coastal land. BAROFD. 9(12): 1-10.

Gupta H, Gupta RC, Kumar R, Singhal VK. 2017. A profile of chromosome counts, male meiosis and pollen fertility in 45 species of Asteraceae from Parvati Valley in Kullu district, Himachal Pradesh. Caryologia 70(2): 128-140.

Heywood VH. 1978. Flowering Plants of the World. Oxford University Press, Oxford, UK, pp. 263-268.

http://www.leicabiosystems.com/specimen-preparation/consumables/mounting-media-section-adhesive/details/product/historesin-1/

Karaduman D, Sağıroğlu M. 2021. Flora of Acarlar Longoz (Floodplain) (Sakarya) and its surroundings. Sakarya Univ J of Sci. 25(1): 252-274.

Kartal Ç. 2015. Microsporogenesis, microgametogenesis and in vitro pollen germination in the endangered species Fritillaria stribrnyi (Liliaceae). Caryologia 68(1): 36-43.

Kartal Ç. 2016. Calcium oxalate crystals in some species of the tribe Cardueae (Asteraceae). Bot Sci. 94(1): 107-119.

Kaur D, Singhal VK, Gupta RC. 2010. Male meiosis, microsporogenesis and pollen grain study in Inula cuspidata CB Clarke (Asteraceae). Cytologia 75(4): 363-368.

Kaur D, Tantray YR, Singhal V K. 2019. Cytological study in some species of Lactuca L. (Asteraceae) from high altitudinal regions of Kinnaur district, Himachal Pradesh, India. Taiwania 64(2): 111.

Kılıç O. 2020. Determination of total phenolic, flavonoid substance, antioxidant and antimicrobial properties of Allium rumelicum, Jurinea kilaea, Peucedanum obtusifolium plants growing in Thrace region. Master’s Thesis, Tekirdağ Namık Kemal University, TURKEY.

Kolczyk J, Tuleja M, Płachno B. 2015. Histological and cytological analysis of microsporogenesis and microgametogenesis of the invasive species Galinsoga quadriradiata Ruiz & Pav. (Asteraceae). Acta Biol Cracov Bot. 57(2): 89-97.

Kutbay HG, Sürmen B, Ağır ŞU, Kılıç DD. 2017. Samsun ili kıyı kumullarında tespit edilen yabancı bitkiler. Turk J Weed Sci. 20(2): 19-27.

Li F, Chen S, Chen F, Teng N, Fang W, Zhang F, Deng Y. 2010. Anther wall development, microsporogenesis and microgametogenesis in male fertile and sterile Chrysanthemum (Chrysanthemum morifolium Ramat., Asteraceae). Sci Hortic (Amsterdam). 126(2): 261-267.

Liu JX, Wang M, Chen BX, Jin P, Li JY, Zeng K. 2012. Microsporogenesis, microgametogenesis, and pollen morphology of Ambrosia artemisiifolia L. in China. Pl Syst Evol. 298: 43-50.

Malik RA, Kumari S. 2010. Genetic diversity in different populations of Artemisia absinthium Linn. from Kashmir Himalaya. Cytologia 75(3): 273-276.

Meriç C, Dane F, Olgun G. 2004. Histological aspects of anther wall in male fertile and cytoplasmic male sterile Helianthus annuus L. (Sunflower) Asian J Plant Sci. 3:145-150.

Morisset P. 1978. Cytomixis in pollen mother cells of Ononis (Leguminosae). Can J Genet Cytol. 20(3): 383-388.

Mursalimov SR, Baiborodin SI, Sidorchuk YV, Shumny VK, Deineko EV. 2010. Characteristics of the cytomictic channel formation in Nicotiana tabacum L. pollen mother cells. Cytol Genet. 44: 14-18.

Mursalimov SR, Sidorchuk YV, Deineko E. 2013. New insights into cytomixis: specific cellular features and prevalence in higher plants. Planta 238: 415–423.

Nikolić M, Stevović S. 2015. Family Asteraceae as a sustainable planning tool in phytoremediation and its relevance in urban areas. Urban For Urban Green. 14: 782–789.

O’Brien T, Feder NMEM, McCully ME.1964. Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma 59: 368-373.

Özhatay N, Akalın E, Güler N, Ersoy H, Yeşil Y, Demirci S. 2013. Floristic richness and conservation priority sites in the northwest of European Turkey: Mt Yıldız-Kırklareli. Phytol Balc. 19(1): 77-88.

Özhatay N, Öztekin M. 2015. Plant biodiversity and unique yew stands of Istranca (Yıldız) mountains in European Turkey. In YEW Workshop 2015. Düzce- Turkey, pp. 76.

Pacini E, Franchi GG, Hesse M. 1985. The tapetum: its form, function, and possible phylogeny in Embryophyta. Pl Syst Evol.149: 155-185.

Papini A, Mosti S, Brighigna L. 1999. Programmed-cell-death events during tapetum development of angiosperms. Protoplasma 207, 213–221.

Rolnik A, Olas B. 2021. The plants of the Asteraceae family as agents in the protection of human health. Int J Mol Sci. 22(6): 3009.

Saday S. 2005. Jurinea Cass. (Compositae) Üzerinde Morfolojik, Palinolojik ve Anatomik Araştırmalar (PhD. Thessis, Marmara Universitesi -Turkey).

Shekhar S, Pandey AK. 2009. Anther appendages and anther collar in Asteraceae and their taxonomic significance. Pleione 3(2): 212-218.

Shi Z et al. [total: 33 co-authors] 2011. Asteraceae (Compositae) [family introduction, glossary, systematic list, and key to tribes]. Pp. 1–8 in: Wu ZY, Raven PH, Hong D Y eds., Flora of China Volume 2021 (Asteraceae). Science Press (Beijing) & Missouri Botanical Garden Press (St. Louis).

Sun M, Ganders FR. 1987. Microsporogenesis in male‐sterile and hermaphroditic plants of nine gynodioecious taxa of Hawaiian Bidens (Asteraceae). Am J Bot. 74(2): 209-217.

Susanna A, Baldwin BG, Bayer RJ, Bonifacino JM, Garcia‐Jacas N, Keeley SC, ... Stuessy TF. 2020. The classification of the Compositae: A tribute to Vicki Ann Funk (1947–2019). Taxon 69(4): 807-814.

Sürmen B, Şenay ULU, Kutbay HG. 2019. Rare dune plant species in Samsun Province, Turkey. Ant J Bot. 3(2): 34-39.

Szukala A, Korotkova N, Gruenstaeudl M, Sennikov AN, Lazkov GA, Litvinskaya SA, ... von Raab‐Straube E. 2019. Phylogeny of the Eurasian genus Jurinea (Asteraceae: Cardueae): Support for a monophyletic genus concept and a first hypothesis on overall species relationships. Taxon 68(1): 112-131.

Taç S, Özcan T. 2019. Fatty acid and amino acid profiles in some dune vegetation species from İstanbul. Süleyman Demirel Univ Fen Bilim Enst Derg. 23(3): 892-903.

Takats ST. 1959. Chromatin extrusion and DNA transfer during microsporogenesis. Chromosoma 10: 430-453.

Tuncay HO, Akalın UE. 2018. Endemism in İstanbul plants. Eur J Biol. 77(1): 38-41.

Uslu M, Keçeli T. 2019. İğneada Longoz Ormanları Milli Parkı (Demirköy-Kırklareli) Ciğerotları (Marchantiophyta) Florası. Anatolian Bryol. 5(2): 114-129.

Valcheva M, Sopotlieva D, Apostolova I. 2020. Current state and historical notes on sand dune flora of the Bulgarian Black Sea Coast. Flora 267: 151594.

Wang CY, Li X, Wu QF, Wang XY. 2006. Cytoplasmic channels and their association with plastids in male meiocytes of tobacco, onion and lily. Cell Biol Int. 30(5): 406-411.

Whelan ED. 1974. Discontinuities in the callose wall, intermeiocyte connections, and cytomixis in angiosperm meiocytes. Canad J Bot. 52(6): 1219-1224.

Wortley AH, Blackmore S, Chissoe WF, Skvarla JJ. 2012. Recent advances in Compositae (Asteraceae) palynology, with emphasis on previously unstudied and unplaced taxa. Grana 51(2): 158-179.

Yıldırımlı Ş. 1999. The chorology of the Turkish species of Asteraceae family. Ot Sist Bot. 6(2): 75-123.

Yurukova-Grancharova P, Dimitrova D. 2006. Cytoembryological study of Crepis bithynica (Asteraceae) from Bulgaria. Flora Mediterr. 16: 33-43.

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Published

2025-10-07

How to Cite

Ekici, N. (2025). Anther structure and pollen development in Jurinea kilaea Azn. (Asteraceae). Caryologia, 78(1), 41–51. https://doi.org/10.36253/caryologia-2965

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