First report of chromosome and karyological analysis of Gekko nutaphandi (Gekkonidae, Squamata) from Thailand: Neo-diploid chromosome number in genus Gekko

Authors

  • Weera Thongnetr Division of Biology, Department of Science, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok 10120
  • Suphat Prasopsin Research Academic Supports Division, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi 71150
  • Surachest Aiumsumang Biology program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000
  • Sukhonthip Ditcharoen Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Khlong Luang, Pathum Thani 12120
  • Alongklod Tanomtong Program of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002
  • Prayoon Wongchantra Center of Environmental Education Research and Training, Faculty of Environment and Resource Studies,Mahasarakham University 44150
  • Wutthisak Bunnaen Mahasarakham University Demonstration School (Secondary) Kham Riang Sub-District, Kantharawichai District, Maha Sarakham, 44150
  • Sumalee Phimphan Phimphan

DOI:

https://doi.org/10.36253/caryologia-1875

Keywords:

Chromosome, Gekko nutaphandi, Karyotype, Red-eyed Gecko

Abstract

The karyotypes of red-eyed Gecko are not reported yet. Herein, we describe the karyotypes of red-eyed Gecko (Gekko nutaphandi Bauer, Sumontha & Pauwels, 2008) from Thailand. Gecko chromosome preparations were directly conducted from bone marrow and testis. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(GC)15 probe. The results showed that the number of diploid chromosomes is 2n=34, while the fundamental number (NF) is 46 in both males and females. The types of chromosomes were 4 large metacentric, 6 large submetacentric, 2 medium telocentric, 2 small metacentric and 20 small telocentric chromosomes. The results of conventional Giemsa staining presented the diploid chromosome number differentiation even in the same genus. NORs are located at the secondary constriction to the telomere on the long arm of chromosome pair 5. There are no sex differences in karyotypes between males and females. FISH with d(GC)15 sequences were also displayed at the telomeres of most other chromosomes. We found that during metaphase I the homologous chromosomes showed synapsis, which can be defined as 19 ring bivalents and 17 haploid chromosomes (n=17) at metaphase II as a diploid species. The karyotype formula is as follows: 2n (34) = L4m+L6sm+M2t+S2m+S20t.

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Published

2023-04-28

How to Cite

Thongnetr, W., Prasopsin, S., Aiumsumang, S., Ditcharoen, S., Tanomtong, A., Wongchantra, P., Bunnaen, W., & Phimphan, S. (2023). First report of chromosome and karyological analysis of Gekko nutaphandi (Gekkonidae, Squamata) from Thailand: Neo-diploid chromosome number in genus Gekko. Caryologia, 75(4). https://doi.org/10.36253/caryologia-1875

Issue

Vol. 75 No. 4 (2022)

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Copyright (c) 2022 Weera Thongnetr, Suphat Prasopsin, Surachest Aiumsumang, Sukhonthip Ditcharoen, Alongklod Tanomtong, Prayoon Wongchantra, Wutthisak Bunnaen, Sumalee Phimphan

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