First cytogenetic study of the Somphong’s rasbora (Trigonostigma somphongsi) (Perciformes, Cyprinidae), a critically endangered species in Thailand
DOI:
https://doi.org/10.36253/caryologia-2439Keywords:
Karyotype, Chromosome, Trigonostigma somphongsiAbstract
The first chromosome study of Trigonostigma somphongsi (Meinken,1958) from Thailand. Specimens were collected from Pak Phli District, Nakhornnayok Province, central Thailand. The mitotic chromosomes were directly prepared from kidney tissues of two males and two females. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results shown that diploid chromosome number of T. somphongsi was 2n=50, the fundamental numbers (NF) were 92 in both male and female. The types chromosomes consisted of 10 metacentric, 20 submetacentric, 12 acrocentric and 8 telocentric chromosomes. The result also exhibited that the interstitial nucleolar organizer regions (NORs) were clearly observed at the short arm of chromosome pair 2. The karyotype formula could be deduced as: 2n (diploid) 50=10m+20sm+12a+8t.
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Aiumsumang S, Phimphan S, Suwannapoom C, Chaiyasan P, Supiwong W, Tanomtong A. 2021. A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand. Caryologia 74(1): 89 –196. https://doi.org/10.36253/caryologia-1017.
Aiumsumang S, Phimphan S, Tanomtong A, Supiwong W. 2022. Chromosome study of Rasbora trilineata and Rasbora borapetensis (Cyprinidae, Cypriniformes): Reveal by conventional staining technique. Science Technology and Engineering Journal 8(2): 29–39.
Almeida-Toledo, LF, Foresti F. Toledo-Filho SA. 1984. Complex sex chromosome system in Eigenmannia sp. (Pisces, Gymnotiformes). Genetica 64: 165–169. https://doi.org/10.1007/BF00115340.
Amemiya CT, Gold JR. 1988. Chromosomal NORs as taxonomic and systematic characters in North American cyprinid fishes. Genetica 76: 81–90. https://doi.org/10.1007/BF00058806.
Barat A, Sahoo PK. 2007. Karyotype analysis of Channa punctatus (Pisces) using restriction endonucleases. Cytologia 72: 471–473. https://doi.org/10.1508/cytologia.72.471.
Barat A, Sahoo PK, Ponniah AG. 2002. Karyotype and Nucleolar Organizer Regions (NORs) in a few hill stream fishes. In: Ayyappan, S., Jena, J. K. and Joseph, M. M. (Eds.), The Fifth Indian Fisheries Forum Proceedings, AFSIB, Mangalore and AoA, Bhubaneswar, pp.111–114.
Beardmore JA, Mair GC, Lewis RI. 2001. Monosex male production in finfish as exemplified by tilapia: applications, problems, and prospects. Aquaculture 197: 283–301. doi:10.1016/S0044-8486(01)00590-7.
Desprez D, Mélard C, Hoareau MC, Belleme`ne Y, Bosc P, Baroiller JF. 2003. Inheritance of sex in two ZZ pseudofemale lines of tilapia, Oreochromis aureus. Aquaculture 218: 131–140. https://doi.org/10.1016/S0044-8486(02)00572-0.
Donsakul T, Magtoon W. 1995. Karyological studies of Puntius brevis (Bleeker, 1850) and Cyclocheilichthys enoplos in Thailand. Proceeding of 21st Congress on Science and Technology of Thailand, pp. 292–293.
Donsakul T, Magtoon W. 2002. Karyotype of Rasbora Caudimaculata, R. myersi, R. retrodorsalis, R. paviei from Thailand. Academic conference seminar for research papers of Srinakharinwirot University (pp. 1–7). Bangkok.
Donsakul T, Magtoon W, Rangsiruji W. 2005. Karyotype of five species of fish (Pla siew) in subfamily Rasboranae. 31st Congress on Science and Technology of Thailand. Suranaree University of Technology. Nakhon Ratchasima Province Thailand.
Donsakul T, Rangsiruji A, Magtoon W. 2009. Karyotypes of five cyprinid fishes (Cyprinidae, DanioninaeDanionini): Rasbora agilis, R. dorsiocellata, R. rubrodorsalis, Boraras maculate and B. urophthalmoides from Thailand. In Proceedings of the 47th Kasetsart University Annual Conference (pp. 320–327). Kasetsart University, Thailand
Galetti Jr PM. 1998. Chromosome diversity in neotropical fish NOR studies. Italian Journal of Zoology. 65: 53–56. https://doi.org/10.1080/11250009809386795.
Gold JR, Li YC, Shipley NS, Powers PK. 1990. Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. Journal of Fish Biology 37: 563–575. https://doi.org/10.1111/j.1095-8649.1990.tb05889.x.
Howell WM, Black DA. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: A 1-step method. Experientia 36: 1014–1015. https://doi.org/10.1007/BF01953855.
Khuda-Bukhsh AR. 1979. Karyology of two species of hillstream fishes, Barilius bendelisis and Rasbora daniconius (Fam: Cyprinidae). Current Sci. 48(17): 793–794.
Manna GK, Khuda-Bukhsh, A.R. 1977. Karyomorphology of cyprinid fishes and cytological evaluation of the family. The Nucleus, 20: 119–127
Petsut N, Panitvong N, Kulabtong S, Petsut J, Nonpayom C. 2014. The first record of Trigonostigma somphongsi (Meinken,1958), a critically endangered species, in its natural habitat of Thailand (Cypriniformes Cyprinidae). Biodiversity Journal 5(4): 471–474.
Post A. 1965. Vergleichede Untersuchungen der chromosomenzahlen bei Susswasser-Teleosteern. In: Gyldenholm, A.O. and Scheel, J.J. Chromosome number of fishes I. Journal Fish Biology, 3: 47–93.
Rooney DE. 2001. Human Cytogenetics: Constutitional analysis: A Practical approach. 3rd ed. London: Oxford University Press.
Seetapan K, Moeikum T. 2004. Karyotypes of ten Cyprinid fishes (Family Cyprinidae). Journal Agricultural Extension, 22: 92–101
Tan HH. 2020. Trigonostigma truncata, a new species of harlequin rasbora from Malay Peninsula (Teleostei: Danionidae). Raff. Bull. Zool. 68: 421–433. https://doi.org/10.26107/RBZ-2020-0058
Turpin R, Lejeune J. 1965. Les Chromosomes Humains. Gauthier Pillars.
Ueda T, Naoi H, Arai R. 2001. Flexibility on the karyotype evolution in bitterlings (Pisces, Cyprinidae). Genetica 111: 423–432. https://doi.org/10.1023/a:1013703717626
Yeesaem N, Jantarat S, Yeesin P. 2019. Cytogenetic Characterigation of Rasbora einthovenii in Sirindhorn Peat Swamp Forest, Narathiwat Province. Journal of Fisheries Technology Research, 13: 58–68.
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